Effect of administrating polysaccharide from black currant (<i>Ribes nigrum</i> L.) on atopic dermatitis in NC/Nga mice

Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease that causes dry skin and functional disruption of the skin barrier. AD is often accompanied by allergic inflammation. AD patient suffer from heavy itching, and their quality of life is severely affected. Some pharmaceuticals for AD have some side effects such as skin atrophy. So it is necessary to develop mild solutions such as food ingredients without side effects. There are various causes of AD. It is especially induced by immunological imbalances such as IFN-γ reduction. IFN-γ has an important role in regulating IgE, which can cause an allergy reaction. NC/Nga mice develop AD and IgE hyperproduction. In a previous study, we revealed that administration of polysaccharide from black currant (R. nigrum) has an effect on immunomodulation. It induces IFN-γ production from myeloid dendritic cells. We named this polysaccharide cassis polysaccharide (CAPS). In this report, we studied the effect of administering CAPS on atopic dermatitis in NC/Nga mice. Thirty NC/Nga mice that developed symptoms of atopic dermatitis were used. We divided them into three groups (control, CAPS administration 12 mg/kg/day, CAPS administration 60 mg/kg/day). For 4 weeks, we evaluated clinical score, serum IgE levels, gene expression of spleen, and skin pathology. We revealed that CAPS administration improves atopic dermatitis symptoms. We also found that CAPS administration suppresses IgE hyperproduction and induces IFN-γ gene transcription in the spleen. Finally, we confirmed that CAPS administration suppresses mast cell migration to epidermal skin. These results indicated that CAPS has an effect on AD.

Orally Administered Polysaccharide Derived from Blackcurrants (<i>Ribes nigrum</i> L.) Improves Skin Hydration in Ultraviolet-Irradiated Hairless Mice

“…We obtained 13.5 g freeze-dried powder from 3,454 g blackcurrant juice; the mean MW was 27,643. This result supports the earlier result that indicates that the freeze-dried powder mainly contains fiber (16).…”

“…Blackcurrant (Ribes nigrum L.) is a well-known healthy fruit that is used to prepare various foods and beverages, such as jams and juices. In previous studies, we revealed that a polysaccharide-rich substance derived from blackcurrants reduces skin inflammation linked to atopic dermatitis, and that it has an anti-tumor effect in mice (14)(15)(16). We designated this substance cassis polysaccharide (CAPS).…”

A Review of the Potential Benefits of Plants Producing Berries in Skin Disorders

“…Moreover, the serum IgE level in the animals treated with cassis polysaccharide at 60 mg/kg was significantly lower compared with the control group. The animals receiving the highest dose of cassis polysaccharide showed a significantly decreased number of mast cells in the epidermis [ 65 ].…”

Blackcurrants (Ribes nigrum): A Review on Chemistry, Processing, and Health Benefits

“…Ashigai et al. () demonstrated the effectiveness of oral intake of BC cassis polysaccharide on reducing skin dehydration caused by ultraviolet light in mice. They also reported decreases on markers of inflammation, such as those of interleukin‐6 and matrix metalloprotein transcription levels in the skin of hairless mice.…”

“…The degraded polysaccharide U-600 W (M w = 1.32 × 10 4 kDa) ultrasound treated sample exhibited the best results for all assays performed when compared to the polysaccharide that received a lower wattage treatment. Ashigai et al (2018) demonstrated the effectiveness of oral intake of BC cassis polysaccharide on reducing skin dehydration caused by ultraviolet light in mice. They also reported decreases on markers of inflammation, such as those of interleukin-6 and matrix metalloprotein transcription levels in the skin of hairless mice.…”