Effect of acid phosphatase produced by Trichoderma asperellum Q1 on growth of Arabidopsis under salt stress

Abstract

Salt stress is a major environmental factor that inhibits crop growth. Trichoderma spp. are the most efficient biocontrol fungi and some of the strains can stimulate plant growth. Phosphate solubilization is known as one of the main mechanisms in promoting plant growth, but the underlying mechanisms of phosphate solubilization in the salinity still need to be explored. The T. asperellum Q1 isolated and identified in our lab is a beneficial rhizosphere biocontrol fungus with a high phosphate solubilization activity. It could produce acid and alkaline phosphatases when using insoluble organic phosphorus as the sole phosphorus source, the salt stress increased the P-solubilization ability of the strain and the activities of the two enzymes. Furthermore, an acid phosphatase was purified from the fermentation broth by ammonium sulphate precipitation, ion-exchange, and gel filtration chromatography. Its molecular weight was 55 kDa as determined by SDS-PAGE. The purified acid phosphatase was used to investigate growth performance of Arabidopsis thaliana by plate assay and the result showed that it contributed to Arabidopsis growth by transforming organic phosphate into a soluble inorganic form under salt stress. To our knowledge, this is the first report of acid phosphatase purification from T. asperellum and its function in regulation of plant growth under salt stress.

NaCl Inhibits Citrinin and Stimulates Monascus Pigments and Monacolin K Production

“…ACP and AKP activities were also enhanced by 0.02 M NaCl treatment in this study. A similar result was presented in Trichoderma asperellum Q1, showing that NaCl could activate ACP and AKP [ 35 ]. In addition, the activity of AKP was enhanced by Na + treatment in Aspergillus caespitosus [ 36 ].…”

Phosphorus-solubilizing Trichoderma spp. from Amazon soils improve soybean plant growth

“…Phosphorus www.nature.com/scientificreports www.nature.com/scientificreports/ that is readily available to the plant is a phosphate anion, a poorly mobile element in the soil and plants compared to other macronutrients. Thus, in addition to transforming organic phosphate into inorganic phosphate, Trichoderma helps to increase the root system, contributing to a greater region of nutrient absorption by the plant 48 . Few reported on the mechanisms of plant-Trichoderma interaction in promoting growth.…”

“…One of the mechanisms of action of Trichoderma for nutrient supplementation of plants is via the production of phosphatase enzymes. Some authors have already described the activity of this fungus in terms of its production of these enzymes 47,48,52,65 . The activity of phosphatases is reported mainly at sites where there is an absence of inorganic phosphorus 52 .…”

CHARACTERIZATIONS OF Trichoderma sp. AND ITS EFFECT ON Ralstonia solanacearum OF TOBACCO SEEDLINGS

“…Fungi released secondary metabolites such as peptaibols and harzianolides (Zeilinger et al, 2016), acid phosphatase that play a role in phosphate solubilization (Zhao et al, 2017) and various other active secondary metabolites to the rhizosphere that promote root branching and nutrient absorption for plants (Martínez-Medina et al, 2014;López-Bucio et al, 2015). The growth and development of root was strongly influenced by the availability of hormones (Giehl & von Wirén, 2014).…”