Dynamics of skin barrier repair following preconditioning by a biotechnology-driven extract from samphire (Crithmum maritimum) stem cells

Abstract

Background With aging, the barrier repair kinetics following any weakening of the epidermal permeability barrier function is commonly slowed down. Objective To assess the recovery rate of the epidermal permeability barrier function following controlled stripping and applications of samphire and control formulations. Method In 12 healthy subjects older than 50 years, controlled stratum corneum (SC) strippings were used to increase the transepidermal water loss (TEWL) just above 15 g ⁄ m 2 ⁄ h. This procedure followed a 14-day skin preconditioning by daily applications of formulations enriched or not with a samphire (Crithmum maritimum) biomass. An untreated skin site served as control. The epidermal permeability repair kinetics was assessed for 14 days by daily measurements of both TEWL and the colorimetric value a*. Results A rapid (96 h) recovery to lower TEWL values was obtained at each of the samphire-preconditioned sites (0.1% serum, 0.05% cream, the serum-cream association, and 0.5% silicone oil). This process was significantly (P < 0.001) faster than that on both the placebo-preconditioned (silicone oil) and the untreated sites. No adverse inflammatory and sensory reactions were recorded. At the sites preconditioned by samphire formulations, the SC moisture (capacitance) was higher at completion of the study compared to inclusion. Conclusions The present experimental pilot study brings some clues supporting a beneficial boosting effect of samphire cell biomass on the kinetics of epidermal permeability barrier repair.

Prediction of skin anti‐aging clinical benefits of an association of ingredients from marine and maritime origins: Ex vivo evaluation using a label‐free quantitative proteomic and customized data processing approach

“…They differ from the differentiated plant cells by their richness in phenolic acids and more specifically in chlorogenic acid derivatives. Previous studies disclosed a positive influence of dedifferentiated sea fennel cells on epidermal regeneration, skin barrier recovery, and the inhibition of melanogenesis . The three active ingredients were formulated in an aqueous gel as described in Table .…”

“…Previous studies disclosed a positive influence of dedifferentiated sea fennel cells on epidermal regeneration, skin barrier recovery, and the inhibition of melanogenesis. 19,20 The three active ingredients were formulated in an aqueous gel as described in Table 1. In brief, glycerin and preservatives were mixed together and added to water.…”

Singlet molecular oxygen quenching by the antioxidant dimethylmethoxy chromanol in solution and in ex vivo porcine skin

“…Furthermore, the integrity of the skin was evaluated by the transepidermal water loss (TEWL) measurement. A TEWL value of 5–10 g/m 2 ·h is taken as an indication of healthy skin and the value of 15 g/m 2 ·h is commonly taken as an acceptable upper limit for skin permeability studies . Thus, only the biopsies with a TEWL value lower than 15 g/m 2 ·h were used in the experiments.…”

Plant stem cells culture — a new tool for skin protection and regeneration

“…This resulted in the growing thickness of the individual skin layers and increased cell density in both the epidermis and dermis [45]. In clinical studies, extract of stem cells from samphire improved epidermal permeability barrier repair and revealed the soothing and moisturizing effect on the skin [47]. The extracts of apple and argan stem cells, in turn, reduced wrinkles appearing around the eyes ("crow's feet") and resulted in an increase of skin density [43,48,49].…”