Abstract:Development of biotechnology, esthetic medicine and cosmetology can enable us to slow down or delay the skin aging process. Currently, much attention is aimed at treatments using substances of plant origin. They have been proven to exhibit antioxidant, antibacterial and antifungal properties, accelerate wound healing, moisturize the skin, enhance skin renewal processes and protect skin against UV radiation. Biologically active plant-derived compounds, however, are often produced by plants in very small amounts… Show more
“…Similar applications are associated at present with in vitro cultured plant stem cells. Currently, the most promising field of plant stem cells is cosmetic manufacture due to the antioxidant and anti-inflammatory properties of stem cell derivatives [ 36 , 56 ]. Plant stem cells can be applied as active stem cells or stem cell extracts, especially in skin care products [ 32 , 57 ].…”
The in vitro cultures of plant stem cells and stem cell-like cells can be established from tissues containing meristematic cells. Chemical compounds—as well as their production potential—is among the emerging topics of plant biotechnology. We induced the callus cell biomass growth and characterized the parameters indicating the presence of stem cells or stem cell-like cells. Four types of explants (stem, petiole, leaf, root) from Sida hermaphrodita (L.) Rusby and various combinations of auxins and cytokinins were tested for initiation of callus, growth of sub-cultivated callus biomass, and establishment of stem cells or stem cell-like cells. Induction of callus and its growth parameters were significantly affected both by the explant type and the combination of used plant growth hormones and regulators. The responsibility for callus initiation and growth was the highest in stem-derived explants containing cambial meristematic cells. Growth parameters of callus biomass and specific characteristics of vacuoles confirmed the presence of stem cells or stem cell-like cells in sub-cultivated callus cell biomass. Establishment of in vitro stem cell or stem cell-like cell cultures in S. hermaphrodita can lead to the development of various applications of in vitro cultivation systems as well as alternative applications of this crop.
“…Similar applications are associated at present with in vitro cultured plant stem cells. Currently, the most promising field of plant stem cells is cosmetic manufacture due to the antioxidant and anti-inflammatory properties of stem cell derivatives [ 36 , 56 ]. Plant stem cells can be applied as active stem cells or stem cell extracts, especially in skin care products [ 32 , 57 ].…”
The in vitro cultures of plant stem cells and stem cell-like cells can be established from tissues containing meristematic cells. Chemical compounds—as well as their production potential—is among the emerging topics of plant biotechnology. We induced the callus cell biomass growth and characterized the parameters indicating the presence of stem cells or stem cell-like cells. Four types of explants (stem, petiole, leaf, root) from Sida hermaphrodita (L.) Rusby and various combinations of auxins and cytokinins were tested for initiation of callus, growth of sub-cultivated callus biomass, and establishment of stem cells or stem cell-like cells. Induction of callus and its growth parameters were significantly affected both by the explant type and the combination of used plant growth hormones and regulators. The responsibility for callus initiation and growth was the highest in stem-derived explants containing cambial meristematic cells. Growth parameters of callus biomass and specific characteristics of vacuoles confirmed the presence of stem cells or stem cell-like cells in sub-cultivated callus cell biomass. Establishment of in vitro stem cell or stem cell-like cell cultures in S. hermaphrodita can lead to the development of various applications of in vitro cultivation systems as well as alternative applications of this crop.
“…V. vinifera is also a research subject in terms of biotechnological studies. There is growing interest in V. vinifera in vitro stem cells as well as tissue cultures [ 1 , 67 ]. It is expected that V. vinifera in vitro culture extracts will be proposed as innovative and effective cosmetic ingredients in the future.…”
Section: Discussionmentioning
confidence: 99%
“…In recent years, natural cosmetics with innovative ingredients have been in demand. The particular interest of the cosmetic industry is focused on V. vinifera stem cells, which have applications mainly in anti-ageing creams and essences [ 1 ]. The Mibelle Biochemistry company (Switzerland) has developed a new biotechnology technique under the name PhytoCellTech™, which is used to generate plant stem cells.…”
Section: The Applications Of
V Vinifera
In Vitro C...mentioning
confidence: 99%
“…Modern phytocosmetology is an extremely prominent field that has recently drawn the attention of research centres. There is an increasing demand for natural cosmetics, the key task of which is to protect the skin from free radicals and oxidative stress with a low risk of side effects [ 1 , 2 ]. Scientists are constantly searching for innovative raw materials with potential applications in different cosmetic formulations [ 3 ].…”
This review refers to botanical, ecological and phytochemical characteristics of Vitis vinifera L. (vine grape)–a species, the valuable properties of which are widely exploited in the food industry and in recent times in medicine as well as in phytocosmetology. The general characteristic of V. vinifera, followed by the chemical composition and biological activities of different extracts obtained from the plant (fruit, skin, pomace, seed, leaf and stem extracts), are provided. A concise review of the extraction conditions of grape metabolites and the methods of their analysis are also presented. The biological activity of V. vinifera is determined by the presence of high contents of polyphenols, mainly flavonoids (e.g., quercetin, kaempferol), catechin derivatives, anthocyanins and stilbenoids (e.g., trans-resveratrol, trans-ε-viniferin). The review pays particular attention to the application of V. vinifera in cosmetology. It has been proven that V. vinifera possesses strong cosmetological-related properties, such as anti-ageing properties, anti-inflammatory properties and skin-whitening properties. Moreover, a review of studies on V. vinifera biological activities, which are of particular interest for dermatologic problems, are disclosed. Furthermore, the work also emphasises the importance of biotechnological studies on V. vinifera. The last part of the review is addressed to the safety of the use of V. vinifera.
“…The antioxidant and regenerative ability of plant stem cells and/or stem cell extracts, make them useful for medical uses. [ 8 ] Medicinal plants/herbs have less toxic effects and are also inexpensive making them a promising treatment modality for periodontal regeneration. [ 1 ]…”
Background:
Periodontitis causes the destruction of soft and hard tissues. Stem cells have immense potential in regenerative cellular therapy. This clinical trial aimed to evaluate clinically and radiographically the effectiveness of the local application of Edelweiss stem cells as a nonsurgical treatment for stage III periodontitis.
Materials and Methods:
The trial included 40 periodontal pockets in participants who have stage III periodontitis with probing pocket depth (PPD) ≥5 mm and clinical attachment loss (CAL) ≥5 mm. Pockets were randomly divided into two groups Group 1: was given oral hygiene instruction, scaling, root planing, and subgingival application of plant stem cells on gel foam carrier after that a periodontal dressing was applied. The procedures were repeated after 2 weeks. Group 2: was treated only by scaling and root planing. Gingival index, CAL, and PPD were measured at baseline and 3 months’ posttherapy. The radiographical evaluation was done by digital long-cone parallel periapical radiographs at baseline and 6 months posttherapy.
Results:
Clinical parameters for both groups showed a statistically significant improvement. Regarding radiographic evaluation, there was a significant increase in bone density in favor of the study group.
Conclusions:
Locally applied Edelweiss stem cells can be considered a promising nonsurgical treatment modality for periodontal regeneration.
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