Dual-Mode Sensing Platform Guided by Intramolecular Electrochemiluminescence of a Ruthenium Complex and Cationic N,N-Bis(2-(trimethylammonium iodide)propylene) Perylene-3,4,9,10-tetracarboxydiimide for Estradiol Assay

Abstract

Herein, a dual-mode sensing platform using cationic N,N-bis­(2-(trimethylammonium iodide)­propylene)­perylene-3,4,9,10-tetracarboxydiimide (PDA+)-assembled DNA strands as a quencher was suggested for estradiol (E2) detection. The aptamer chain was initially anchored with the Ru­(II) novel molecule (Ru complex), which was recombined with carbohydrazide (CON4H6) and tris­(4,4′-dicarboxylicacid-2,2′-bipyridyl)­ruthenium­(II) dichloride [Ru­(dcbpy)3 2+] modified on copper oxide (CuO) nanospheres. Intramolecular electrochemiluminescence (ECL) occurring between CON4H6 and Ru­(dcbpy)3 2+ effectively improved the reaction rate and increased the ECL efficiency. By employing effective van der Waals’ force, PDA+ was endowed with an efficient ECL quenching probe on an electrode. The signal on the ECL interface can be converted into quenching because of energy transfer between the intercalator and the emitter. Notably, cationic PDA+ possessing a large planar π–π skeleton improved advantageous activity of redox and DNA aptamer indurative loading capacity and directly generated a well-defined cathodic peak to execute the EC bio-detection. This method not only avoids the difficulty of assembling various signal indicators but also improves the sensitivity greatly using the quenching mechanism. In addition, disparate double-response signals coming from different principles of transduction are in a position to verify each other to improve the accuracy. Hence, examination areas of 0.001–100 nM with E2 for ECL and EC were obtained, supplying a novel sensing strategy with promising ideas and perspectives of detection platform construction.

RuSiO2@Ag Core–Shell Nanoparticles for Plasmon Resonance Energy Transfer-Based Electrochemiluminescence Sensing of Glucose and Adenosine Triphosphate

“…The ECL spectra of RuSiO 2 (donor) with a maximum emission at 595 nm partly overlapped with the UV–vis spectrum of AgNPs (acceptor) with a maximum wavelength at 452 nm, which could provide the possibility of the ECL-RET. , The lowest unoccupied molecular orbital (LUMO) and the highest occupied molecular orbital (HOMO) energy levels (E LUMO or E LUMO ), shown in Scheme A, were detected and calculated (Figure S2). As displayed by the results, E LUMO of RuSiO 2 (−3.17 eV) was higher than that of AgNPs (−3.70 eV), which also provides the possibility of the ECL-RET. , Photoluminescence (PL) characterization of the mixture of RuSiO 2 and AgNPs (corresponding characterizations are shown in Figure S3A) was provided to further prove the RET between the RuSiO 2 and AgNPs (Figure S3B). The PL intensity was gradually decreased following the increase in the concentration of AgNPs, revealing that there exists energy transfer between RuSiO 2 and AgNPs …”

“…As described in Figure 4A, under optimal experimental conditions (shown in Figures S5 and S6), the ECL intensity dose-dependently increased when the concentration of Glu was gradually increased. The ECL intensity was linear to the logarithmic concentration of Glu from 0.2 to 20 μM (Figure 4B) with a linear equation of I = 6160.0 + 6446.0 lg c. The limit of detection (LOD) 31 was determined to be 0.15 μM. Compared with other ECL sensors for the detection of Glu, the present sensor displayed advantages of a wider linear range or lower LOD (Table S1).…”

“…As displayed by the results, E LUMO of RuSiO 2 (−3.17 eV) was higher than that of AgNPs (−3.70 eV), which also provides the possibility of the ECL-RET. 31,32 Photoluminescence (PL) characterization of the mixture of RuSiO 2 and AgNPs (corresponding characterizations are shown in Figure S3A) was provided to further prove the RET between the RuSiO 2 and AgNPs (Figure S3B). The PL intensity was gradually decreased following the increase in the concentration of AgNPs, revealing that there exists energy transfer between RuSiO 2 and AgNPs.…”

Biomaterials and advanced technologies for the evaluation and treatment of ovarian aging

“…In recent years, methods for quantitative detection of serum estrogen have emerged. The main products were enzyme-linked immunosorbent assay [ 110 ], fluorescence immunoassay [ 111 ], chemiluminescence immunoassay [ 112 ] and electrochemiluminescence immunoassay [ 113 ]. Conventional immunoassay techniques have been under scrutiny for some time with their selectivity, accuracy and precision coming into question [ 114 ].…”

Molecularly Imprinted Polymer Functionalized Bi2S3/Ti3C2TX MXene Nanocomposites for Photoelectrochemical/Electrochemical Dual-Mode Sensing of Chlorogenic Acid

“…Thus, there is an urgent need for a separate signal that possesses a totally independent transmission path, which improves the accuracy and sensitivity of the proposed strategy. In recent years, a number of dual-mode biological detection strategies have been reported, in which two independent signal transductions from different mechanisms can calibrate the systematic errors or background signals, and mutually verify dual responses, such as electrochemiluminescence (ECL)-electrochemical (EC) [8], ECL-colorimetry [9], PEC-fluorescence [10], PEC-colorimetry [11] and chemiluminescence-PEC strategies [12]. In these dual-mode platforms, the PEC-EC strategy requires only one electrochemical instrument to achieve two signal readouts on the same probe, optimizing the sensing interface and reducing uncontrollable changes.…”