RuSiO₂@Ag Core–Shell Nanoparticles for Plasmon Resonance Energy Transfer-Based Electrochemiluminescence Sensing of Glucose and Adenosine Triphosphate

Abstract

Convenient and fast approaches for sensitive monitoring of different biomolecules are of vital importance for obtaining insights into biological processes and related diseases. Herein, silica nanospheres encapsulated with Ru­(bpy)3 2+ (RuSiO2) were uniformly coated with silver nanoparticles (AgNPs) through the in-situ reduction method to form RuSiO2@Ag core–shell nanoparticles, which were employed to fabricate a versatile electrochemiluminescence (ECL) sensing system for the assay of glucose (Glu) and adenosine triphosphate (ATP) on the basis of ECL resonance energy transfer (ECL-RET). RuSiO2@Ag core–shell nanoparticles showed high RET efficiency due to the combination of the donor (RuSiO2)–acceptor (AgNPs) pair into one nanostructure, causing a significantly quenched ECL signal of RuSiO2. Afterward, the Glu oxidase immobilized on RuSiO2@Ag by DNA complementation naturally catalyzed the oxidation of Glu to produce H2O2, which then triggered the etching of AgNPs. Consequently, the remarkably recovered ECL signal was further used to specifically detect Glu and achieved a limit of detection of 0.15 μM. ATP was chosen as a model molecule to verify the detecting ability of the sensor and achieved femtomolar-level results. The versatile ECL sensing system based on enzymatic oxydate-triggered etching and RET strategies hold potential promise for the convenient and sensitive detection of multiple types of biomolecules.

Zinc Porphyrin Mixed with Metal Organic Framework Nanocomposites and Silver Nanoclusters for the Electrochemiluminescence Detection of Iodide

“…Electrochemiluminescence (ECL) has attracted wide attention in food detection, bioanalysis, and clinical applications due to low background signals, low operation cost, and strong controllability. − Traditional ECL luminophore has advantages of high luminous efficiency . However, it typically has drawbacks such as high biotoxicity and difficult process of synthesis .…”

Aggregation-Induced Electrochemiluminescence Based on a Zinc-Based Metal–Organic Framework and a Double Quencher Au@UiO-66-NH2 for the Sensitive Detection of Amyloid β 42 via Resonance Energy Transfer

“…Electrochemiluminescence (ECL) occurs due to electrochemical and chemiluminescence reactions on electrode surfaces or near the surface of the electrode. ECL has attracted wide attention for clinical diagnosis and drug analysis due to its low background, high sensitivity, and high controllability. − Several novel ECL concepts have emerged in the last few years. Aggregation-induced emission (AIE) is an interesting phenomenon discovered by Tang and his colleagues that overcomes the obstacle due to aggregation-caused quenching (ACQ). − To prevent ACQ, Tang and his colleagues modified ACQ nuclei with AIE molecules to distort the structure of the ACQ nuclei and destroy the molecular planarity to suppress π–π stacking.…”

LuMA-Functionalized Thermosensitive Hydrogel: A Versatile and Robust Dopamine-Triggered Platform for Diverse Biomolecules Sensing

“…Inspired by fluorescence resonance energy transfer (RET), ECL-RET has been paid much more attention due to its enhanced sensitivity, low background interference, and broad dynamic range. , The good spectrum overlap and the nanometer-scale distance between the donor–acceptor pair are the essential prerequisites for ECL-RET. , To improve the RET efficiency, the donor–acceptor pairs have been shifted from the solution to the surface of the electrode. Specific interaction of antigen–antibody, affinity binding of aptamer-target, and the encapsulation of the donor and acceptor into one nanostructure have been explored to shorten the distance between the donor and acceptor. − Besides, other strategies for the high ECL-RET system construction based on the short donor–acceptor distance were proposed. , The suitable ECL-RET donor–acceptor pairs have been investigated and employed for the assay of different biomolecules in the past few years. The most common luminophores, ruthenium­(II) complexes, have been reported as versatile acceptors of different donors including luminol, g-C 3 N 4 nanosheet, and CdZnSe@ZnSe quantum dots. − As another traditional luminescent reagent, luminol and its derivatives have been extensively served as ECL luminophores because of their high emission yield, low oxidation potential, and good stability .…”