Downregulation of microRNA‑660 inhibits cell proliferation and invasion in osteosarcoma by directly targeting forkhead box O1

Zhang, Peng; Gao, Haifeng; Li, Qing; Chen, Xinlei; Wu, Xifa

doi:10.3892/mmr.2018.9165

Cited by 8 publications

(9 citation statements)

References 35 publications

(43 reference statements)

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“…MiR-215-5p and miR-642a-5p may be potential biomarkers for diagnosis of osteosarcoma [19]. And miR-660 promotes osteosarcoma cell proliferation and invasion [20].…”

Section: Discussionmentioning

confidence: 99%

miR-627-3p inhibits osteosarcoma cell proliferation and metastasis by targeting PTN

Shen

Qiu

et al. 2019

Aging

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Dysregulation of microRNA (miRNA) has been observed in several types of tumors, including osteosarcoma. Biochip analysis was used to identify miRNAs differentially expressed in osteosarcoma tissues. The targeting sites of miR-627-3p were analyzed using miRDB software and fluorescein reporter gene. MTT and Transwell assays were used to analyze the effects of miR-627-3p on the growth and migration of osteosarcoma cells. Western blotting and real-time PCR were used to detect the effects of miR-627-3p on related proteins. In vivo experiments were conducted to verify the effect of miR-627-3p on osteosarcoma. We focused on miR-627-3p because it was the most significantly downregulated miRNA in our screening study. Through luciferase reporter assays, western blotting and real-time PCR we found that miR-627-3p directly targets PTN, and that expression levels of miR-627-3p and PTN are negatively correlated in osteosarcoma cells. Downregulation of miR-627-3p promoted osteosarcoma cell proliferation and metastasis, while its overexpression had the opposite effect. By targeting PTN, miR-627-3p also suppressed expression of Cyclin D1 and MMP2. MiR-627-3p inhibited osteosarcoma metastasis in vivo. Thus, miR-627-3p may be a useful therapeutic target for the treatment osteosarcoma or prevention of metastasis.

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“…MiR-215-5p and miR-642a-5p may be potential biomarkers for diagnosis of osteosarcoma [19]. And miR-660 promotes osteosarcoma cell proliferation and invasion [20].…”

Section: Discussionmentioning

confidence: 99%

miR-627-3p inhibits osteosarcoma cell proliferation and metastasis by targeting PTN

Shen

Qiu

et al. 2019

Aging

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“…At the same time, studies have shown that the miRNA-34 family (miRNA-34a, miRNA-34b, and miRNA-34c) can also affect multiple target genes including the Notch signaling pathway, thereby affecting the pathogenesis of OS [12]. In addition, Zhang et al [13] studies have also shown that miRNA-34c can not only play a carcinogenic role by inhibiting the expression of the Notch signaling pathway, but also directly inhibit the normal differentiation of osteoblasts, leading to a cell cycle regulation disorder. Other reduced-expressing miRNAs, such as miRNA-218, were also reduced significantly in human OS tissues and OS cell lines (SAOS-2 cells), and transfection of miRNA-218 gives SAOS-2 cells the ability to invade and metabolize distantly.…”

Section: Mirna and Os Occurrence And Developmentmentioning

confidence: 99%

Advances in the role of miRNAs in the occurrence and development of osteosarcoma

Zhang

et al. 2020

Open Medicine

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Osteosarcoma (OS) is the most common primary malignant tumor of the skeletal system in the clinic. It mainly occurs in adolescent patients and the pathogenesis of the disease is very complicated. The distant metastasis may occur in the early stage, and the prognosis is poor. MicroRNAs (miRNAs) are non-coding RNAs of about 18–25 nt in length that are involved in post-transcriptional regulation of genes. miRNAs can regulate target gene expression by promoting the degradation of target mRNAs or inhibiting the translation process, thereby the proliferation of OS cells can be inhibited and the apoptosis can be promoted; in this way, miRNAs can affect the metabolism of OS cells and can also participate in the occurrence, invasion, metastasis, and recurrence of OS. Some miRNAs have already been found to be closely related to the prognosis of patients with OS. Unlike other reviews, this review summarizes the miRNA molecules closely related to the development, diagnosis, prognosis, and treatment of OS in recent years. The expression and influence of miRNA molecule on OS were discussed in detail, and the related research progress was summarized to provide a new research direction for early diagnosis and treatment of OS.

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“…Micrornas (mirnas/mirs), are short, noncoding single-stranded rnas that regulate gene expression, and their roles as regulators of Bn pathogenesis has been reported (6,7). For example, one study found that mir-367-5p downregulation promoted the proliferation of osteoblasts in a model of microgravity-induced bone healing.…”

Section: Introductionmentioning

confidence: 99%

Identification of key microRNAs and target genes for the diagnosis of bone nonunion

et al. 2020

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a number of recent studies have highlighted the causes of bone nonunion (Bn), however, the rate of Bn incidence continues to rise and available therapeutic options to treat this condition remain limited. Thus, to prevent disease progression and improve patient prognosis, it is vital that BN, or the risk thereof, be accurately identified in a timely manner. in the present study, bioinformatics analyses were used to screen for the differentially expressed genes (deGs) and differentially expressed mirnas (deMs) between patients with Bn and those with bone union, using data from the Gene expression omnibus database. Furthermore, clinical samples were collected and analyzed by reverse transcription-quantitative Pcr and western blotting. In vitro and in vivo experiments were carried out to confirm the relationship between Bn and the deGs of interest, in addition to being used to explore the underlying molecular mechanism of Bn. Functional enrichment analysis of the downregulated deGs revealed them to be enriched for genes associated with 'ecM-receptor interactions', 'focal adhesion', 'and the calcium signaling pathway'. When comparing deM target genes with these DEGs, nine DEGs were identified as putative deM targets, where hsa-microrna (mir)-1225-5p-ccnl2, hsa-mir-339-5p-PrcP, and hsa-mir-193a-3p-mitogen-activated protein kinase 10 (MaPK10) were the only three pairs which were associated with decreased gene expression levels. Furthermore, hsa-mir-193a-3p was demonstrated to induce Bn by targeting MaPK10. collectively, the results of the present study suggest that hsa-mir-193a-3p may be a viable biomarker of Bn.

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Downregulation of microRNA‑660 inhibits cell proliferation and invasion in osteosarcoma by directly targeting forkhead box O1

Cited by 8 publications

References 35 publications

miR-627-3p inhibits osteosarcoma cell proliferation and metastasis by targeting PTN

miR-627-3p inhibits osteosarcoma cell proliferation and metastasis by targeting PTN

Advances in the role of miRNAs in the occurrence and development of osteosarcoma

Identification of key microRNAs and target genes for the diagnosis of bone nonunion

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