Do spawn storage conditions influence the colonization capacity of a wheat-straw-based substrate by Agaricus subrufescens?

Abstract

Storage conditions of the spawn of edible fungi are of major importance to facilitate the production of mushrooms. Here, standard storage conditions at 10°C or 15°C were used and the potential of colonization of standard European compost by the tropical species Agaricus subrufescens was assessed during the spawn running phase. Two lignocellulolytic activities, laccase and CMC-cellulase, were enhanced after storage compared to control as well as substrate transformation, as described by the aromaticity ratio and a humification ratio calculated from NMR data. This result indicates that mycelium growth probably occurred during storage at 10 or 15°C, leading to a larger amount of biomass in the inoculum. Moreover, the microbial functional diversity of the substrate was favored, showing that the electivity of the substrate was maintained. Thus, these findings indicate that recommendations for the mushroom producers can be established for A. subrufescens cultivation under European standard conditions.

Impact of Cultivation Substrate and Microbial Community on Improving Mushroom Productivity: A Review

“…This method of measuring the metabolism of different carbon sources was used to evaluate the effect of spawn storage conditions on the substrate colonization of A. subrufescens. The results from the study reveal that the storage conditions of relatively low temperatures for up to 30 days did not affect the substrate colonization of this mushroom species [100]. This method exhibited higher sensitivity and simplicity than traditional plate culture.…”

Using of Appropriated Strains in the Practice of Compost Supplementation for Agaricus subrufescens Production

“…An emerging Agaricus species, Agaricus subrufescens Peck, also named Agaricus blazei Murrill sensu Heinemann, Agaricus rufotegulis Nauta, or Agaricus brasiliensis Wasser, M. Didukh, Amazonas & Stamets, has been an actively cultivated mushroom in Brazil since 90's; when it was cultivated for first time in the Sao Paulo State and then extended to other regions close to the Atlantic coast (Farnet et al, 2014).…”

Preparation of a sugarcane bagasse-based substrate for second-generation ethanol: Effect of pasteurisation conditions on dephenolisation

“…Laccases were quantified according to Farnet et al [27], with syringaldazine (15 mM in methanol) as substrate. Cellulase activity was assayed using Carboxy-Methylcellulose (CMC) at 1% as substrate according to the Somogyi-Nelson method modified by Farnet et al, [28].…”