DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis

Forster, Karine M.; Hartwig, Daiane Drawanz; Oliveira, Thaís Larré; Bacelo, Kátia Leston; Schuch, Rodrigo Andrade; Amaral, Marta; Dellagostin, Odir A.

doi:10.1590/0074-02760150222

Cited by 20 publications

(22 citation statements)

References 42 publications

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“…This indicated that L. interrogans Piscina may pose a risk to public health in the region ( Forster et al 2013 ). Due to its high virulence, this isolate has also been used as a challenge strain in experiments aimed to evaluate the protection afforded by novel recombinant vaccine candidates against leptospirosis in vaccinology laboratories ( Forster et al 2015 ).…”

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confidence: 99%

Whole-genome sequencing of Leptospira interrogans from southern Brazil: genetic features of a highly virulent strain

Jorge

Kremer

Oliveira

et al. 2018

Mem. Inst. Oswaldo Cruz

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BACKGROUNDLeptospirosis is the most widespread zoonotic disease. It is caused by infection with pathogenic Leptospira species, of which over 300 serovars have been described. The accurate identification of the causative Leptospira spp. is required to ascertain the pathogenic status of the local isolates.OBJECTIVESThis study aimed to obtain the complete genome sequence of a virulent Leptospira interrogans strain isolated from southern Brazil and to describe its genetic features.METHODSThe whole genome was sequenced by next-generation sequencing (Ion Torrent). The genome was assembled, scaffolded, annotated, and manually reviewed. Mutations were identified based on a variant calling analysis using the genome of L. interrogans strain Fiocruz L1-130 as a reference.FINDINGSThe entire genome had an average GC content of 35%. The variant calling analysis identified 119 single nucleotide polymorphisms (SNPs), from which 30 led to a missense mutation. The structural analyses identified potential evidence of genomic inversions, translocations, and deletions in both the chromosomes.MAIN CONCLUSIONSThe genome properties provide comprehensive information about the local isolates of Leptospira spp., and thereby, could facilitate the identification of new targets for the development of diagnostic kits and vaccines.

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confidence: 99%

Whole-genome sequencing of Leptospira interrogans from southern Brazil: genetic features of a highly virulent strain

Jorge

Kremer

Oliveira

et al. 2018

Mem. Inst. Oswaldo Cruz

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“…The level of the immune response induced by heterologous immunization is higher and more balanced than single immunization, and the protective effect is also improved. This strategy is expected to reduce the times of immunization compared with immunization with an inactivated vaccine alone, and theoretically, it can also improve the immunogenicity and long-term immune response induced by the vaccine [ 21 , 22 ], which warrants further experiments.…”

Section: Discussionmentioning

confidence: 99%

Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in China

Zheng

Wang

et al. 2020

Virol J

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Background: The incidence of Japanese encephalitis (JE) has been dramatically reduced in China after sufficient vaccine coverage. The live-attenuated Japanese encephalitis virus (JEV) vaccine SA14-14-2 is believed to have strongly contribute to this decrease. Another vaccine that seems to have decreased in importance is an inactivated vaccine based on the JEV P3 strain, which is considered to be modifiable, such as being transformed into a DNA vaccine to improve its immunogenicity. Methods: In this study, the protective efficacy induced by the Japanese encephalitis DNA vaccine candidate pV-JP3ME encoding the premembrane (prM) and envelope (E) proteins of the P3 strain was assessed in BALB/c mice. The prM/E genes of the JEV P3 strain were subcloned into the vector pVAX1 (pV) to construct pV-JP3ME. Results: The plasmid DNA was immunized into BALB/c mice, and high titers of IgG antibody and neutralizing antibody (nAb) against JEV were detected. The key cytokines in splenocytes were secreted upon stimulation with JEV antigens. Finally, complete protective efficacy was generated after challenge with the JEV P3 strain in the mice. Conclusions: The DNA vaccine pV-JP3ME based on the JEV P3 strain in this study can induce specific humoral immune and cytokine responses and provide complete protection against JEV in mice.

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“…The level of the immune response induced by heterologous immunization is higher, more balanced, and the protective effect is also improved. This idea is expected to reduce the times of immunization compared with immunizing an inactivated vaccine alone, and theoretically, it can also improve the immunogenicity and prolong the duration of the vaccine [20,21], which warrants later experiments.…”

Section: Discussionmentioning

confidence: 99%

Complete protection for mice conferred by a DNA vaccine based on Japanese encephalitis virus P3 strain that is used to prepare the inactivated vaccine in China

Wang

et al. 2020

Preprint

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Background The incidence of Japanese encephalitis (JE) has been dramatically reduced in China after the coverage of the vaccine. It is believed that the live-attenuated Japanese encephalitis virus (JEV) vaccine SA14-14-2 has contributed a lot. Another vaccine that seems to have faded out of the public is an inactivated vaccine based on the JEV P3 strain, which is still considered to have certain modifiability, such as being transformed into a DNA vaccine to improve its immunogenicity. Methods In this study, the protective efficacy induced by a Japanese encephalitis DNA vaccine candidate pV-JP3ME encoding pre-membrane (prM) and envelope (E) proteins of P3 strain in BALB/c mice. The prM/E genes of the JEV P3 strain were subcloned into vector pVAX1 (pV) to construct pV-JP3ME. Results The plasmid DNA was immunized BALB/c mice, high titers of IgG antibody and neutralizing antibody (nAb) against JEV were detected. The key cytokines in splenocytes upon stimulation with JEV antigens were secreted. Finally, complete protective efficacy was generated after challenge with the JEV P3 strain in mice. Conclusions The DNA vaccine pV-JP3ME based on JEV P3 strain in this study can induce specific humoral immune and cytokine responses in mice, and provide complete protection for mice against JEV.

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DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis

Cited by 20 publications

References 42 publications

Whole-genome sequencing of Leptospira interrogans from southern Brazil: genetic features of a highly virulent strain

Whole-genome sequencing of Leptospira interrogans from southern Brazil: genetic features of a highly virulent strain

Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in China

Complete protection for mice conferred by a DNA vaccine based on Japanese encephalitis virus P3 strain that is used to prepare the inactivated vaccine in China

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