BACKGROUNDLeptospirosis is the most widespread zoonotic disease. It is caused by
infection with pathogenic Leptospira species, of which over
300 serovars have been described. The accurate identification of the
causative Leptospira spp. is required to ascertain the
pathogenic status of the local isolates.OBJECTIVESThis study aimed to obtain the complete genome sequence of a virulent
Leptospira interrogans strain isolated from southern
Brazil and to describe its genetic features.METHODSThe whole genome was sequenced by next-generation sequencing (Ion Torrent).
The genome was assembled, scaffolded, annotated, and manually reviewed.
Mutations were identified based on a variant calling analysis using the
genome of L. interrogans strain Fiocruz L1-130 as a
reference.FINDINGSThe entire genome had an average GC content of 35%. The variant calling
analysis identified 119 single nucleotide polymorphisms (SNPs), from which
30 led to a missense mutation. The structural analyses identified potential
evidence of genomic inversions, translocations, and deletions in both the
chromosomes.MAIN CONCLUSIONSThe genome properties provide comprehensive information about the local
isolates of Leptospira spp., and thereby, could facilitate
the identification of new targets for the development of diagnostic kits and
vaccines.
Leptospirosis is a zoonotic disease caused by pathogenic spirochetes from the genus Leptospira, which includes 20 species and more than 300 serovars. Canines are important hosts of pathogenic leptospires and can transmit the pathogen to humans via infected urine. Here, we report the phenotypic and molecular characterization of Leptospira interrogans isolated from Canis familiaris in Southern Brazil. The isolated strain was characterized by variable-number tandem-repeats analysis as L. interrogans, serogroup Icterohaemorrhagiae. In addition, the isolate was recognized by antibodies from human and canine serum samples previously tested by microscopic agglutination test. Ultimately, the expression of membrane-associated antigens (LipL32 and leptospiral immunoglobulin-like proteins) from pathogenic leptospires using monoclonal antibodies was detected by indirect immunofluorescence assay. In conclusion, identification of new strains of Leptospira can help in the diagnosis and control of leptospirosis.
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