DNA Fingerprint of <i>Carica papaya</i> L. Genotypes by RAPD Markers

Vitória, Ângela Pierre; Filho, Gonçalo A. de Souza; Bressan-Smith, Ricardo; Pinto, Fernanda Oliveira; Paiva, Luisa Brito; Guimarães, Paula Souza; Oliveira, Michelle de Paula Abreu de; Daher, Rogério Figueiredo; Pereira, Messias Gonzaga

doi:10.1300/j153v06n01_04

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…Some studies on papaya have reported intra-and inter-generic analysis using morphological data, isozyme, RFLP (restriction fragment length polymorphism), PCR-based molecular markers like AFLP (amplified fragment length polymorphism), RAPD (random amplified polymorphic DNA), ISSR (inter-simple sequence repeat), DAMD (directed amplification of minisatellite DNA), and microsatellites or SSR (simple sequence repeats) (Jobin-Decor et al, 1997;Parasnis et al, 1997;Van Droogenbroeck et al, 2002;Vitória et al, 2004;Kyndt et al, 2005;Saxena et al, 2005;Ocampo et al, 2006;Silva et al, 2007;Eustice et al, 2008;Oliveira et al, 2010). However, studies that use morpho-agronomic and molecular data together to assess the genetic material used in breeding programs are scarce.…”

Section: Introductionmentioning

confidence: 99%

Multivariate analysis to determine the genetic distance among backcross papaya (Carica papaya) progenies

Ramos

Pereira²,

Gonçalves

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Morpho-agronomic and molecular (RAPD and ISSR markers) data were used to evaluate genetic distances between papaya backcross progenies in order to help identify agronomically superior genotypes. Thirty-two papaya progenies were evaluated based on 15 morpho-agronomic characteristics, 20 ISSR and 19 RAPD primers. Manhattan, Jaccard and Gower distances were used to estimate differences based on continuous and binary data and combined analyses, respectively. Except for production, there were significant differences in the continuous variables among the genotypes. The molecular analysis revealed 193 dominant markers (ISSR and RAPD), being 53 polymorphic loci. Among the various clusters that were generated, the one based on a combined analysis of morpho-agronomic and molecular data gave the highest cophenetic correlation (0.72) compared to individual Multivariate analysis to estimate divergence in papaya analysis, consistently allocating the progenies into six groups. We found that the Gower algorithm was more coherent in the discrimination of the genotypes, demonstrating that a combination of molecular and agronomic data is valuable for studies of genetic dissimilarity in papaya.

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Section: Introductionmentioning

confidence: 99%

Multivariate analysis to determine the genetic distance among backcross papaya (Carica papaya) progenies

Ramos

Pereira²,

Gonçalves

et al. 2012

Genet. Mol. Res.

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“…Com base em resultados anteriores (VITÓRIA et al, 2004) e triagem de primers, foram selecionados 15 primers: OPO15, OPO10, OPAH18, OPAI03, OPAG11, OPAH14, OPD20, OPS12, OPB02, OPF12, OPG10, OPN09, OPE06, OPAA17 e OPAB11.…”

Section: Reação De Polimerase Em Cadeia (Pcr)unclassified

Genetic diversity in two species of Caricaceae and their genetic relationship toCarica papayaL.

Junior¹,

Pereira²,

Silva³

et al. 2015

Revista Ciência Agronômica

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-The species Vasconcellea monoecious and Jacaratia spinosa are wild germplasm belonging to the Caricaceae family, and are useful in breeding programs of the cultivated species, Carica papaya. The aim of this work was to study the genetic relationships between the three species, and understand the genetic diversity within wild species, using RAPD markers. Accesses belonging to the Caricaceae Germplasm Collection of the Plant Breeding Laboratory of the North Fluminense Federal University (UENF) were collected in two different states of Brazil, Rio de Janeiro and Espirito Santo, and genotyped using molecular markers. The results indicated that the wild species were genetically relatively distant from the cultivated species, and also very distant from each other. This suggests that there may be great difficulty in obtaining interspecific hybrids between these species. The estimates of diversity obtained here demonstrated that the species J. spinosa was superior to V. monoecious. The results showed low genetic variability among the accessions for each species analysed, indicating that insertion of new genetic material into the Caricaceae Germplasm Collection of UENF is essential.

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“…This results in differential amplification of regions that vary in primer site sequence resulting in polymorphic amplification products usually analyzed as presence/ absence. These markers have been previously assayed in the molecular characterization of Carica papaya cultivars [15][16][17][18] and some wild related species [14,19,20], the identification of flower sex types [8,[21][22][23][24][25] and development of genetic linkage maps [26]. In addition, to improve the capacity of the molecular characterization assays using these PCR-based markers, multiplex PCR, a variant of the PCR in which more than one target sequence is amplified using more than one pair of primers are being assayed [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Cuban endemism Carica cubensis Solms using random amplified polymorphic DNA (RAPD) markers

Rodríguez¹,

González²,

Martínez-Gómez³

2010

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The objective of this work is to present an appropriate set of RAPD (random amplified polymorphic DNA) markers using single and multiplex PCR analysis suitable for the characterization of the endemic Cuban species Carica cubensis and the establishment of genetic relationships with the cultivated species Carica papaya. RAPD markers presented a high level of polymorphism. In addition, the incorporation of more than one RAPD primer in the PCR analysis increased the number of obtained bands and the polymorphism of these bands. A total of 73 RAPD bands were detected (45 of them polymorphic) with the nine RAPD markers assayed using single and multiplex PCR analysis. Results demonstrated a reduced genetic variability within the tested Carica cubensis accessions. The observed clustering in this species could be better explained according to geographic proximity and can indicate the similar precedence of the isolated studied populations. C. cubensis seem to be subspecies of C. papaya adapted to the environmental conditions of the mountains of Cuba or a endemic species close to C. papaya. The implications of these results in the creation of effective germplasm core collection in Carica species have been also discussed

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DNA Fingerprint of Carica papaya L. Genotypes by RAPD Markers

Cited by 5 publications

References 12 publications

Multivariate analysis to determine the genetic distance among backcross papaya (Carica papaya) progenies

Multivariate analysis to determine the genetic distance among backcross papaya (Carica papaya) progenies

Genetic diversity in two species of Caricaceae and their genetic relationship toCarica papayaL.

Molecular characterization of Cuban endemism Carica cubensis Solms using random amplified polymorphic DNA (RAPD) markers

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