-This research was done to study the reproductive system of papaya hermaphrodite plant based on the histochemical nature of pollen grain, stigma receptivity, in vivo pollen grain germination and pollen:ovule ratio. In the histochemical analysis, pollen grains were stained by using Sudan IV and I 2 KI solution; the stigma receptivity was assessed by alpha-naphthtyl acetate solution in closed and opened flowers. Pollen germination and pollen tube growing were examined in flower buds near anthesis with 0.1% aniline blue. To estimate the pollen:ovule ratio , anthers from each flower bud were dissected and all pollen grains were counted; ovules were dissected from ovaries and were counted under stereomicroscope. The results indicated that papaya pollen grains are of lipidic nature; the stigmas were receptive before the opening and until 48 hours after opening; the pollen grains germinated and emitted polinic tube before flower opening and the pollen:ovule ratio indicated the predominance of autogamous reproductive system. These results indicate that hermaphrodite papaya trees is preferentially of optional autogamous with cleistogamy. Index terms: Carica papaya, cleistogamy, autogamy, pollen grain histochemistry, P:O ratio, stigma receptivity. MODO DE REPRODUÇÃO PREFERENCIAL DE PLANTAS HERMAFRODITAS DE MAMOEIRO (Carica papaya L; Caricaceae)RESUMO -Esta pesquisa teve o objetivo de estudar o sistema reprodutivo preferencial de plantas hermafroditas de mamoeiro, com base na natureza histoquímica dos grãos de pólen, receptividade do estigma, teste de germinação in vivo do grão de pólen e razão pólen : óvulo. Na análise histoquímica dos grãos de pólen, foram utilizados os corantes Sudan IV e solução de I 2 KI; a receptividade do estigma foi avaliada com a solução de acetato de alfa-naftil em flores abertas e fechadas. A germinação e o crescimento do tubo polínico in vivo foram avaliados em flores fechadas, utilizando solução de azul de anilina a 0,1%. Para estimar a razão pólen:óvulo, anteras de cada flor foram dissecadas, e os grãos de pólen foram contados; ovários foram dissecados, e os óvulos foram contados sob estereomicroscópio. Os resultados indicaram que os grãos de pólen do mamoeiro são de natureza lipídica; os estigmas estavam receptivos antes da abertura e até 48 h após a abertura; os grãos de pólen germinaram e emitiram tubo polínico antes da abertura floral, e a relação pólen:óvulo, indicou predominância do sistema reprodutivo autógamo. Esses resultados indicaram que o modo de reprodução preferencial do mamoeiro hermafrodita é autógamo facultativo com cleistogamia. Termos para indexação: Carica papaya, cleistogamia, autogamia, histoquímica de grãos de pólen, relação P : O, receptividade de estigma.
-The species Vasconcellea monoecious and Jacaratia spinosa are wild germplasm belonging to the Caricaceae family, and are useful in breeding programs of the cultivated species, Carica papaya. The aim of this work was to study the genetic relationships between the three species, and understand the genetic diversity within wild species, using RAPD markers. Accesses belonging to the Caricaceae Germplasm Collection of the Plant Breeding Laboratory of the North Fluminense Federal University (UENF) were collected in two different states of Brazil, Rio de Janeiro and Espirito Santo, and genotyped using molecular markers. The results indicated that the wild species were genetically relatively distant from the cultivated species, and also very distant from each other. This suggests that there may be great difficulty in obtaining interspecific hybrids between these species. The estimates of diversity obtained here demonstrated that the species J. spinosa was superior to V. monoecious. The results showed low genetic variability among the accessions for each species analysed, indicating that insertion of new genetic material into the Caricaceae Germplasm Collection of UENF is essential.
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