DNA barcoding facilitates description of unknown faunas: a case study on Trichoptera in the headwaters of the Tigris River, Iraq

Geraci, Christy J.; Al-Saffar, Mohammed A.; Zhou, Xin

doi:10.1899/10-011.1

Cited by 25 publications

(17 citation statements)

References 24 publications

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“…Regarding Trichoptera, DNA barcoding has been used in numerous studies in different regions (for example Geraci et al 2011;Hjalmarsson et al, 2018;Morinière et al, 2017;Pauls et al 2010;Valladolid et al, 2018Valladolid et al, , 2019Zhou et al, 2016) and that approach has been also applied in Croatia (for example Ćukušić, 2019;Ćukušić et al, 2017;Kučinić et al, 2013Kučinić et al, , 2019aKučinić et al, , 2019bSzivák et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Springs: DNA barcoding of caddisflies (Insecta, Trichoptera) in Croatia with notes on taxonomy and conservation biology

Kučinić

Ćukušić

Žalac³

et al. 2020

Nat. Croat.

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Section: Introductionmentioning

confidence: 99%

Springs: DNA barcoding of caddisflies (Insecta, Trichoptera) in Croatia with notes on taxonomy and conservation biology

Kučinić

Ćukušić

Žalac³

et al. 2020

Nat. Croat.

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“…Moreover, despite the growing need for taxonomic expertise to support ecosystem assessments, the number of well‐trained taxonomists is decreasing (New, ; Stribling, Moulton, & Lester, ; Wheeler, ). In this context, DNA barcoding provides an effective way to overcome the difficulties in morphological identifications, as this technology delivers fast, efficient and reliable species identification (Hausmann, Parisi, & Sciarretta, ; Hausmann et al., ; Hebert, Cywinska, Ball, & DeWaard, ; Hebert, Ratnasingham, & de Waard, ; Miller, Hausmann, Hallwachs, & Janzen, ; Vane‐Wright, Smith, & Kitching, ), even in areas where only little information on the benthic macroinvertebrate fauna is present (Geraci, Al‐Saffar, & Zhou, ; Ibrahimi, Kučinić, Gashi, & Kotori, ). A well‐curated, comprehensive DNA barcode library based upon voucher species is the foundation for such applications (Ball, Hebert, Burian, & Webb, ; Boumans & Brittain, ; Gattolliat, Cavallo, Vuataz, & Sartori, ; Kjaerstad, Webb, & Ekrem, ; Ruiter, Boyle, & Zhou, ; Salokannel, Rantala, & Wahlberg, ; Vuataz, Sartori, Wagner, & Monaghan, ; Webb et al., ; Zhou et al., , , ), as it also enables promising future applications such as environmental DNA barcoding (Baird & Hajibabaei, ; Carew, Pettigrove, Metzeling, & Hoffmann, ; Hajibabaei, Shokralla, Zhou, Singer, & Baird, ; Hajibabaei, Spall, Shokralla, & van Konynenburg, ; Shokralla, Spall, & Gibson, ) and metabarcoding (Gibson et al., ; Leray & Knowlton, ; Morinière et al., ; Yu et al., ), based on high‐throughput sequencing (HTS).…”

Section: Introductionmentioning

confidence: 99%

A DNA barcode library for Germany′s mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera)

Morinière

Hendrich

Balke

et al. 2017

Molecular Ecology Resources

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Mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera) are prominent representatives of aquatic macroinvertebrates, commonly used as indicator organisms for water quality and ecosystem assessments. However, unambiguous morphological identification of EPT species, especially their immature life stages, is a challenging, yet fundamental task. A comprehensive DNA barcode library based upon taxonomically well-curated specimens is needed to overcome the problematic identification. Once available, this library will support the implementation of fast, cost-efficient and reliable DNA-based identifications and assessments of ecological status. This study represents a major step towards a DNA barcode reference library as it covers for two-thirds of Germany's EPT species including 2,613 individuals belonging to 363 identified species. As such, it provides coverage for 38 of 44 families (86%) and practically all major bioindicator species. DNA barcode compliant sequences (≥500 bp) were recovered from 98.74% of the analysed specimens. Whereas most species (325, i.e., 89.53%) were unambiguously assigned to a single Barcode Index Number (BIN) by its COI sequence, 38 species (18 Ephemeroptera, nine Plecoptera and 11 Trichoptera) were assigned to a total of 89 BINs. Most of these additional BINs formed nearest neighbour clusters, reflecting the discrimination of geographical subclades of a currently recognized species. BIN sharing was uncommon, involving only two species pairs of Ephemeroptera. Interestingly, both maximum pairwise and nearest neighbour distances were substantially higher for Ephemeroptera compared to Plecoptera and Trichoptera, possibly indicating older speciation events, stronger positive selection or faster rate of molecular evolution.

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“…Some of those cryptic species which were initially almost impossible to separate using morphological characters alone, have had their identities corroborated by other characters in their natural history and even characters in their morphology (Hebert et al, 2004;Smith et al, 2006;Pfenninger et al, 2007;Decaëns & Rougerie, 2008;Vaglia et al, 2008;Wheat & Watt, 2008;Dasmahapatra et al, 2010;Hausmann et al, 2011. Morphological differences, cases of sexual dimorphism, different castes, or different stages of development have made barcode sequences applicative (Miller et al, 2005;Geraci et al, 2011);Jinbo et al, 2011). Other applications include : identification of host plants by sequencing the stomach contents or plant tissues left on the outside of an insect's body (Jurado- Rivera et al, 2009); identification of the stomach contents of predators in biological control studies (Greenstone et al, 2005); Greenstone (2006); additional data uncovering trophic relationships (Clare et al, 2009;Hrcek et al, 2011); and finally, population genetics, community ecology and biodiversity inventories (Hajibabaei et al, 2006;Lukhtanov et al, 2009;Craft et al, 2010).…”

Section: Dna Barcoding Of Insect Faunamentioning

confidence: 99%

An overview of molecular identification of insect fauna with special emphasis on chalcid wasps (Hymenoptera: Chalcidoidea) of India

Rasool

Ahmad

Ganai

et al. 2018

AAS

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Identifying organisms has grown in importance as we monitor the biological effects of global climate change and attempt to preserve species diversity in the face of accelerating habitat destruction. Classical taxonomy falls short in this race to catalogue biological diversity before it disappears. Differentiating subtle anatomical differences between closely related species requires the subjective judgment of highly trained specialists -and few are being trained in institutes today. DNA barcodes allow non-experts to objectively identify species -from small, damaged, or even industrially processed material. The aim of DNA barcoding is to establish a shared community resource of DNA sequences commonly used for identification, discrimination or taxonomic classification of organisms. It is a method that uses a short genetic marker in an organism's DNA to identify and distinguish its belonging from particular species, varieties or inter varieties. This simple technique has attracted attention from taxonomists, ecologists, conservation biologists, agriculturists, plant-quarantine officers and studies using the DNA barcode has rapidly increased. The extreme diversity of insects and their economical, epidemiological and agricultural importance have made them a major target of DNA barcoding. In this review, we present an overview of DNA barcoding of insects with emphasis on Chalcid wasps of India. Določanje organizmov pridobiva na pomenu pri spremljanju globalnih podnebnih sprememb in pri poskusih ohranjanja biodiverzitete v procesu hitrega uničevanja habitatov. Klasična taksonomija v teh procesih ne uspe določiti vse biodiverzitete pred njenim propadom. Prepoznavanje majhnih anatomskih razlik med ozko sorodnimi vrstami zahteva presojo visoko usposobljenih specialistov, ki jih je danes vedno manj. Vrednotenje DNK zaporedij omogoča tudi nestrokovnjakom objektivno prepoznavanje vrst kot tudi njihovih malih ali poškodovanih ostankov ali celo industrijsko predelanih materialov. Namen te metode je ustvariti nabor DNK zaporedij za vzajemno rabo pri določanju in taksonomskem razvrščanju organizmov poznano tudi pod imenom DNK črtne kode. Pri tej metodi omogoča kratek genetski marker v DNK organizma njegovo določitev in razlikovanje od drugih vrst, različic. Ta preprosta tehnika je pritegnila pozornost taksonomov, ekologov, konzervatorskih biologov, agronomov, fitokarantenskih uradnikov in preučevanje na osnovi sekvenciranja DNK je hitro poraslo. Izjemna raznolikost žuželk in njihov ekonomski, epidemiološki in kmetijski pomen so jih naredile za tarčno skupino preučevanj na osnovi DNK črtnih kod. V tem sestavku predstavljamo pregled analiz z DNK črtnimi kodami žuželk s poudarkom na osicah najezdnicah iz Indije.

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DNA barcoding facilitates description of unknown faunas: a case study on Trichoptera in the headwaters of the Tigris River, Iraq

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 25 publications

References 24 publications

Springs: DNA barcoding of caddisflies (Insecta, Trichoptera) in Croatia with notes on taxonomy and conservation biology

Springs: DNA barcoding of caddisflies (Insecta, Trichoptera) in Croatia with notes on taxonomy and conservation biology

A DNA barcode library for Germany′s mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera)

An overview of molecular identification of insect fauna with special emphasis on chalcid wasps (Hymenoptera: Chalcidoidea) of India

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