Springs: DNA barcoding of caddisflies (Insecta, Trichoptera) in Croatia with notes on taxonomy and conservation biology

Biodiversity, DNA barcoding data and ecological traits of caddisflies (Insecta, Trichoptera) in the catchment area of the Mediterranean karst River Cetina (Croatia)

“…Faunistically, the most interesting species in the study area are: R. d. plitvicensis, G. discophorum, H. vichtaspa, A. sexmaculata, T. minor, E. ivkae, L. sparsus, P.nigricornis, H. infumatu, Leptocerus interruptus, T. lefkas, O. lacustris (Graf et al 2008a;Kučinić et al 2020aKučinić et al , 2020bMalicky, 2014a;Previšić et al 2014;Vučković, 2011;Waringer et al 2009). The species A. sexmaculata, T. minor, L. sparsus, P. nigricornis, H. infumatus, L. interruptus and O. lacustris were recorded for the first time in the Mediterranean part of Croatia, and the Cetina River has been identified as the westernmost point of the distribution area of the species T. lefkas (Kučinić et al 2020a).…”

“…A very interesting finding was the species G. discophorum, confirmed by DNA barcoding, recorded at the springs of the Cetina, the Rumin, the Ruda and the Grab rivers. There were certain indications (morphological characteristics of adults) that the spring of the Ruda River is inhabited by the species G. neretvae, but the analysis of collected material for DNA barcoding in the Cetina River catchment area, and of specimens of G. neretvae collected at the type locality in Bosnia & Herzegovina at the Neretva River, showed that this was undoubtedly the species G. discophorum (Kučinić et al 2020b). This is visible from the maximum likelihood phylogram (Fig 5), which shows that G. discophorum and G. neretvae are forming different cluster as well as two distinct groups identified in ABGD analysis (Group 3 G. discophorum and Group 4 G. neretvae) with interspecific difference ranging from 6.4% to 6.7%.…”

“…Detailed biodiversity, taxonomy, distribution, life cycles, seasonal dynamics and other ecological features were systematically studied in the area of Plitvice Lakes NP (Kučinić 2002;Kučinić & Malicky 2002;Kučinić et al 2017;Marinković-Gospodnetić 1971, 1979Previšić et al 2007a;Šemnički et al 2012), the Cetina River (Graf et al 2008a;Previšić et al 2014;Vučković et al 2016;Waringer et al 2009), the Krka River (Kučinić et al 2011;Ridl et al 2015); Drava River (Previšić et al 2007b), the Gorski kotar region (Cerjanec et al 2020;Ibrahimi et al 2012;Previšić & Popijač 2010) and the Banovina region (Kučinić et al 2010(Kučinić et al , 2013(Kučinić et al , 2020b.…”

“…200 caddisfly species have been recorded (Ćuk & Vučković 2009(Ćuk & Vučković , 2010Graf et al 2008a;Kučinić 2002;Kučinić & Ilić 1993a, 1993bKučinić & Malicky 2002;Kučinić et al 1999Kučinić et al , 2000Kučinić et al , 2011Malicky 2009;Malicky & Krušnik 1988;Malicky et al 2007;Previšić & Popijač 2010;Previšić et al 2007aPrevišić et al , 2007bPrevišić et al , 2014Urbanič et al 2000;Waringer et al 2009). In the last few years the DNA barcoding method has been included in many studies of the caddisfly fauna of Croatia (Ćukušić, 2019;Ćukušić et al 2017;Kučinić et al 2013Kučinić et al , 2019aKučinić et al , 2019bKučinić et al , 2019cKučinić et al , 2020aKučinić et al , 2020b The objectives of this paper were to determine faunistic features of caddisflies (1), caddisfly ecology and distribution in the study area (2) and give a note on results of the DNA barcoding of some species (3).…”

DNA barcoding and a new taxonomic status of the Triaenodes ochreellus lefkas Malicky, 1974 (Insecta, Trichoptera) with new distribution data

“…Brehm et al 2019;Cárdenas et al 2013;Elías-Gutiérrez et al 2008;Guo et al 2016;Huemer et al 2020;Léger et al 2020;Tyagi et al 2017;Yang et al 2016), including Trichoptera (Kučinić et al 2016(Kučinić et al , 2017(Kučinić et al , 2019aSzivák et al 2017;Santos et al 2016;Valladolid et al 2018Valladolid et al , 2019Vitecek et al 2020). In many studies, DNA barcoding has aided morphological identification of different taxa (Ćukušić et al 2017;Kučinić et al 2019bKučinić et al , 2020, but also enabled the discovery of new species (Brehm et al 2019;Dela Cruz et al 2016;Graf et al 2012;Kučinić et al 2013;Léger et al 2020;Tyagi et al 2017;Vaglia et al 2008). Although there are no generally accepted values for genetic differences within DNA barcode region among various species, for animals it is considered that an intraspecific genetic distance of more than 2% between populations is high intraspecific variability within the same species (Hebert et al 2003b).…”