Diversidade genética entre híbridos de tangerina 'Cravo' e laranja 'Pêra'

Oliveira, R. P. de; Cristofani, M; Aguilar-Vildoso, Carlos Ivan; Machado, Marcos Antônio

doi:10.1590/s0100-204x2002000400008

Cited by 12 publications

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“…O coeficiente de Jaccard tem sido utilizado nos estudos de diversidade genética em plantas (Jakše et al, 2001;Oliveira et al, 2002a). Em milho, a comparação de diferentes coeficientes de similaridade demonstrou que o tipo de marcador molecular utilizado em análises de similaridade pode causar alterações nas análises e interpretações dos resultados (Meyer et al, 2004).…”

Section: Resultsunclassified

“…Marcadores monomórficos para a progênie não foram considerados na análise, conforme Oliveira et al (2002a). Cada banda foi considerada um único locus genético, não levando em consideração possível alelismo entre diferentes tipos de AFLP, conforme utilizado por Meyer et al (2004).…”

Section: Methodsunclassified

“…Marcadores moleculares possibilitam a avaliação da similaridade genética entre genótipos nos primeiros estágios de desenvolvimento, e são vantajosos para esse tipo de estudo em plantas perenes, como os citros (Nicolosi et al, 2000). Em cruzamentos dirigidos de citros, informações sobre as relações genéticas entre os híbridos podem orientar a seleção de materiais promissores e o entendimento da herança de diversos caracteres de interesse, e são fundamentais para o melhoramento (Bastianel et al, 1998;Oliveira et al, 2002a).…”

Section: Introductionunclassified

“…Em citros, marcadores RAPD (Random Amplified Polymorphic DNA) têm sido utilizados em estudos de diversidade genética (Machado et al, 1995;Bastianel et al, 1998;Nicolosi et al, 2000;Oliveira et al, 2002a;Andrade et al, 2004). Marcadores AFLP (Amplified Fragment Lenght Polymorphism), por sua vez, são considerados mais eficientes que RFLP (Restriction Fragment Lenght Polymorphism), RAPD e SSR (Single Sequence Repeat), pela sua alta reprodutibilidade e elevada taxa multiplex, ou seja, número de marcadores simultaneamente analisados em um mesmo gel, e vêm sendo utilizados para análise de diversidade genética em muitas espécies vegetais (Pejic et al, 1998).…”

Section: Introductionunclassified

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Diversidade genética entre híbridos de laranja-doce e tangor 'Murcott' avaliada por fAFLP e RAPD

Bastianel

Oliveira

Cristofani

et al. 2006

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a diversidade genética em uma população de 148 híbridos de tangor 'Murcott' (Citrus reticulata Blanco x C. sinensis L. Osbeck) e laranja 'Pêra' (C. sinensis L. Osbeck) obtidos por polinização controlada, pelo uso de marcadores fAFLP e RAPD. Marcadores polimórficos (416 marcadores fAFLP e 33 RAPD) foram utilizados para avaliar a similaridade genética entre os híbridos, calculada com o coeficiente Jaccard pelo método UPGMA. A consistência de cada agrupamento foi determinada pelo programa BOOD. Houve alta similaridade genética entre os parentais. A laranja 'Pêra' apresentou maior número (132) de loci em heterozigose em relação ao tangor 'Murcott' (105), corroborando a teoria de origem híbrida para a laranja-doce. Observaram-se dois grupos distintos de plantas, e um deles abrangeu 80% dos híbridos com maior similaridade com a laranja 'Pêra'. A análise bootstrap não revelou consistência estatística entre esses grupos. Marcadores fAFLP são mais eficientes na avaliação do polimorfismo, sendo indicados para seleção de indivíduos híbridos mais próximos a um dos parentais.Termos para indexação: citros, marcadores moleculares, similaridade genética. Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markersAbstract -The objective of this work was to evaluate the genetic diversity in a population of 148 hybrids of 'Murcott' tangor (Citrus reticulata Blanco x C. sinensis L. Osbeck) and 'Pêra' sweet orange (C. sinensis L. Osbeck), obtained by controlled polination, using fAFLP and RAPD markers. Polymorphic markers (416 fAFLP and 33 RAPD markers) were used to evaluate genetic similarity among the hybrids, calculated by the coefficient of Jaccard, using the UPGMA method. The consistency of each group was determined by software BOOD. There was high genetic similarity within the parents. 'Pêra' sweet orange had a higher number of loci in heterozygosis (132) compared to 'Murcott' tangor (105), supporting the theory of hybrid origin for sweet oranges. Two distinct groups of plants were observed: one group had 80% of the hybrids that displayed higher genetic similarity with 'Pêra' sweet orange. The bootstrap analysis did not show consistence between the groups. The fAFLP markers are more efficient to obtain polymorphism than RAPDs and are more suitable to select hybrids closer to the parents.

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Section: Resultsunclassified

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Diversidade genética entre híbridos de laranja-doce e tangor 'Murcott' avaliada por fAFLP e RAPD

Bastianel

Oliveira

Cristofani

et al. 2006

Pesq. agropec. bras.

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“…The DNA extraction and the RAPD analyses were conducted according to Oliveira et al [16]. Ninety-seven decamer primers of arbitrary sequences were used from the following Operon Technologies Inc. (Alameda, CA, USA) kits: A, AB, AT, AV, B, C, D, E, G, H, I, M, N, P, Q, R, U and Y.…”

Section: Dna Extraction and Rapd Assaymentioning

confidence: 99%

Integrated genetic map of citrus based on RAPD markers

Cristofani

Machado²

2005

Fruits

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Integrated genetic map of citrus based on RAPD markers.Abstract --Introduction. The citrus industry is one of the main activities of Brazilian agriculture. Several pests and diseases have threatened citrus culture in recent years. Genetic mapping is one of the most efficient strategies for conducting advanced genetic studies by facilitating plant selection guided by markers. The objective of this study was to construct an integrated genetic map between Citrus sinensis (L.) Osbeck cv. 'Pêra' and C. reticulata Blanco cv. 'Cravo' using two different types of segregation of markers. Materials and methods. The linkage analyses were conducted with segregation data obtained from 256 RAPD markers in a population of 94 hybrids. Analyses were performed through the software JoinMap, LOD ≥ 6.0, θ ≤ 0.25, and the Kosambi function. Results and discussion. The linked map between the two cultivars had 217 markers defined by 15 linkage groups, covering 527 cM. This map had five linkage groups with common markers of 'Pêra' and 'Cravo'. The inclusion of new markers would increase the number of linkage groups with markers from both parents, thus making their number equal to the haploid chromosome number. The alterations in the order and in the distances among the markers, due to the presence or absence of bridging markers in the linkage map, are discussed. Citrus reticulata / Citrus sinensis / genetic maps / genetic markers / RAPDCarte génétique intégrée des agrumes, basée sur l'utilisation de marqueurs RAPD.Résumé --Introduction. L'industrie des agrumes est l'une des activités principales de l'agriculture brésilienne. Plusieurs ravageurs et maladies ont menacé cette culture ces dernières années. La cartographie moléculaire est l'une des stratégies les plus efficaces pour entreprendre des études génétiques avancées afin de faciliter une sélection des plants assistée par marqueurs. L'objectif de cette étude a été de construire une carte génétique intégrée entre Citrus sinensis (L.) Osbeck cv. 'Pêra' et C. reticulata Blanco cv. 'Cravo' en utilisant deux types différents de ségrégation des marqueurs. Matériel et méthodes. Les analyses de liaisons ont été conduites à partir de données de ségrégation obtenues de 256 marqueurs RAPD dans une population de 94 hybrides. Les analyses ont été réalisées avec le logiciel JoinMap. Résultats et discussion. La carte des liaisons entre l'un et l'autre des cultivars a comporté 217 marqueurs définis par 15 groupes de liaisons, couvrant 527 cM. Cette carte a présenté cinq groupes de liaisons contenant les marqueurs communs de Pêra et Cravo. L'inclusion de nouveaux marqueurs augmenterait le nombre de groupes de liaisons portant des marqueurs des deux parents, en rendant leur nombre égal au nombre de chromosomes haploïde. Les différences d'ordre et de distances au sein des marqueurs, dues à la présence ou à l'absence de marqueurs dans la carte de liaisons, sont discutées. Citrus reticulata

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Elucidating Genetic Diversity among Sour Orange Rootstocks: a Comparative Study of the Efficiency of RAPD and SSR Markers

Lamine

Mliki

2015

Appl Biochem Biotechnol

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In order to compare the effectiveness of two molecular marker systems, a set of six RAPD and nine SSR markers were used to study the genetic diversity in a population of 46 sour orange accessions, a common rootstock used in almost all citrus orchards in Tunisia. Genetic diversity parameters [average and effective number of alleles, percentage of polymorphism, polymorphic information content (PIC), effective marker index (EMI), and marker index (MI) parameters] for RAPD, SSR, and RAPD + SSR were determined in order to assess the efficiency of the two marker systems. The results revealed that these parameters were significantly higher when using RAPD markers. Similarly, cluster analysis using the results of RAPD was practically the same as that obtained when combining data from the two marker systems (RAPD + SSR) demonstrating the efficiency of RAPD in discriminating between sour orange accessions. Therefore, the use of SSR markers, known to be more efficient and discriminatory, does not bring significant supplementary information in this work. Indeed, results would have been obtained using only the RAPD markers. Accordingly, this work highlights the efficiency and advantages of RAPD, as an easy and efficient technique, in studying citrus rootstock's genetic diversity, and establishing genetic relationships among citrus accessions.

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Diversidade genética entre híbridos de tangerina 'Cravo' e laranja 'Pêra'

Cited by 12 publications

References 13 publications

Diversidade genética entre híbridos de laranja-doce e tangor 'Murcott' avaliada por fAFLP e RAPD

Diversidade genética entre híbridos de laranja-doce e tangor 'Murcott' avaliada por fAFLP e RAPD

Integrated genetic map of citrus based on RAPD markers

Elucidating Genetic Diversity among Sour Orange Rootstocks: a Comparative Study of the Efficiency of RAPD and SSR Markers

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