Divergência genética entre cultivares de gérbera utilizando marcadores RAPD

Rezende, Rodrigo Kelson Silva; Paiva, Luciano Vilela; Paiva, Patrícia Duarte de Oliveira; Chalfun-Júnior, Antônio; Torga, P. P.; Masetto, Tathiana Elisa

doi:10.1590/s0103-84782009005000176

Cited by 13 publications

(7 citation statements)

References 10 publications

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“…With the binary matrix, we calculated the percentage of polymorphisms obtained per primer and the genetic diversity of the locus or PIC (polymorphism information content), which is an estimate used for the evaluation of the discriminatory power of a locus. To calculate the PIC of each primer, we used the equation proposed by Rezende et al (2009). The genetic dissimilarity estimate between each pair of individuals was calculated by the Jaccard coefficient, using the arithmetic complement.…”

Section: Statistical Analysesmentioning

confidence: 99%

Genetic diversity among Hymenaea courbaril L. genotypes naturally occurring in the north of Mato Grosso State, Brazil

Rocha¹,

Tiago²,

Tiago³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Hymenaea courbaril L. is a tropical tree species with economic and medicinal potential largely used in programs for the recovery of degraded areas. The use of molecular markers in genetic studies can 2 V.D. Rocha et al. Genetics and Molecular Research 16 (3): gmr16039706 help in the definition of forest restoration strategies. This study was conducted to analyze the genetic diversity among H. courbaril ('jatobá') genotypes naturally occurring in the north of Mato Grosso State, Brazil, using ISSR markers, aiming to contribute to the development of seed collection and seedling production strategies. Twenty-four H. courbaril individuals were sampled by collecting leaves for DNA extraction by the CTAB method. The individuals were genotyped using 14 ISSR primers, resulting in the amplification of 87 bands in total, 65 of which were polymorphic. The ISSR markers presented average polymorphism information content of 0.44. Dissimilarity values based on the Jaccard coefficient ranged from 0.12 to 0.52. The most divergent individuals were 3 and 22, whereas 14 and 19 were the most genetically similar. The UPGMA and Tocher clustering methods indicated the formation of four distinct groups, revealing genetic variability among the 24 H. courbaril genotypes. Therefore, all the evaluated genotypes can be used for seed collection and seedling production, but the most divergent individuals should be prioritized.

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Section: Statistical Analysesmentioning

confidence: 99%

Genetic diversity among Hymenaea courbaril L. genotypes naturally occurring in the north of Mato Grosso State, Brazil

Rocha¹,

Tiago²,

Tiago³

et al. 2017

Genet. Mol. Res.

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“…The banding profiles showed a total of 42 polymorphic and 7 monomorphic bands from twelve accessions of gerbera. The results show that RAPD is a fast, relatively inexpensive and useful technique for genetic divergence characterization between different cultivars of G. jamesonii (Rezende et al 2009). …”

Section: Discussionmentioning

confidence: 91%

Evaluation of genetic diversity in different genotypes of Gerbera jamesonii Bolus using random amplified polymorphic DNA (RAPD) markers

Priyanka¹,

Singh²,

N.³

et al. 2014

Afr. J. Biotechnol.

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Genetic diversity within Gerbera jamesonii Bolus is the key to genetic improvement of this important ornamental species. In the present study, genetic diversity of 12 accessions of gerbera was assessed through random amplified polymorphic DNA (RAPD) markers. A total of 40 RAPD primers belonging to OPD, OPE, OPF and OPG series of universal primers set were used out of which, only 10 primers produced clear, reproducible and scorable bands. Ten (10) decamer RAPD primers produced a total of 49 scorable bands from 12 genotypes of G. jamesonii Bolus, out of which 42 were polymorphic and seven were monomorphic. The percentage of polymorphism ranged from a maximum of 100.00% by OPE-02, OPE-14, OPF-18, OPG-18, OPG-16 and OPG-17 to a minimum of 50.00% by OPE-08. The RAPD profiles generated were further evaluated for studying the Jaccard's similarity coefficient. The average genetic similarity coefficient for the 12 accessions evaluated by Jaccard index was 0.66 ranging from 0.35 to 0.86. The RAPD amplification data were used to obtain similarity matrix and for generation of dendrogram using unweighted pair group method with arithmetic averages (UPGMA) method. Based on the dendrogram, all 12 genotypes could be distinctly divided into two clusters and the accession CF Orange was found most dissimilar from other accessions. This study shows that DNA based molecular marker RAPD is a powerful, less time consuming and cost effective molecular technique for assessment of genetic diversity among different genotypes of G. jamesonii Bolus. The availability of these gerbera RAPD markers would facilitate the use of molecular markers in gerbera breeding and genetic studies.

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“…where the information of the primer pij is the frequency of the allele p of the locus i, in the j primer (Rezende et al, 2009). PIC represents the existence of variability, which will be higher with higher values of PIC.…”

Section: Discussionmentioning

confidence: 99%

Morphological descriptors and ISSR molecular markers in the evaluation of genetic variability of Tectona grandis genotypes

et al. 2017

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ABSTRACT. This study aimed to evaluate the genetic variability of the teak germplasm bank, using morphological traits and inter-simple sequence repeat molecular markers. Thirty clones were evaluated in a randomized complete block design with three replicates, and each plot was composed of three plants. A joint analysis of quantitative and qualitative variables was performed using the Gower algorithm. Quantitative, qualitative, and molecular variables were analyzed simultaneously using the Ward-MLM procedure. There is genetic variability among the 30 teak genotypes studied, considering the quantitative, qualitative, and molecular variables by the Ward-MLM statistical procedure. Morphological traits used proved to be efficient for the study of genetic variability; however, it was not possible to compose a descriptor table for clonal teak genotypes based on the traits evaluated. The Gower method was efficient in discriminating the groups, demonstrating that the simultaneous analysis of qualitative and quantitative data is feasible and can allow greater efficiency in the knowledge of the variability among teak genotypes. The genotype 22 showed to be the most divergent compared to the other genotypes, except for the cluster of genotypes by the UPGMA method based on the Gower distance obtained by the Ward-MLM procedure, which formed a group with genotypes 9 and 30, in the morphological and molecular analyses and was grouped alone.

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Divergência genética entre cultivares de gérbera utilizando marcadores RAPD

Cited by 13 publications

References 10 publications

Genetic diversity among Hymenaea courbaril L. genotypes naturally occurring in the north of Mato Grosso State, Brazil

Genetic diversity among Hymenaea courbaril L. genotypes naturally occurring in the north of Mato Grosso State, Brazil

Evaluation of genetic diversity in different genotypes of Gerbera jamesonii Bolus using random amplified polymorphic DNA (RAPD) markers

Morphological descriptors and ISSR molecular markers in the evaluation of genetic variability of Tectona grandis genotypes

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