Evaluation of genetic diversity in different genotypes of Gerbera jamesonii Bolus using random amplified polymorphic DNA (RAPD) markers

Abstract

Genetic diversity within Gerbera jamesonii Bolus is the key to genetic improvement of this important ornamental species. In the present study, genetic diversity of 12 accessions of gerbera was assessed through random amplified polymorphic DNA (RAPD) markers. A total of 40 RAPD primers belonging to OPD, OPE, OPF and OPG series of universal primers set were used out of which, only 10 primers produced clear, reproducible and scorable bands. Ten (10) decamer RAPD primers produced a total of 49 scorable bands from 12 genotypes of G. jamesonii Bolus, out of which 42 were polymorphic and seven were monomorphic. The percentage of polymorphism ranged from a maximum of 100.00% by OPE-02, OPE-14, OPF-18, OPG-18, OPG-16 and OPG-17 to a minimum of 50.00% by OPE-08. The RAPD profiles generated were further evaluated for studying the Jaccard's similarity coefficient. The average genetic similarity coefficient for the 12 accessions evaluated by Jaccard index was 0.66 ranging from 0.35 to 0.86. The RAPD amplification data were used to obtain similarity matrix and for generation of dendrogram using unweighted pair group method with arithmetic averages (UPGMA) method. Based on the dendrogram, all 12 genotypes could be distinctly divided into two clusters and the accession CF Orange was found most dissimilar from other accessions. This study shows that DNA based molecular marker RAPD is a powerful, less time consuming and cost effective molecular technique for assessment of genetic diversity among different genotypes of G. jamesonii Bolus. The availability of these gerbera RAPD markers would facilitate the use of molecular markers in gerbera breeding and genetic studies.

Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars

“…3). The estimates of MI were found to be the highest for primer URP9R (4.39) and the lowest with the primer Our study using three marker systems suggested the presence of a significant polymorphism and revealed high level of variability in surveyed gerbera genotypes which agrees with those reported by Prajapati (2014). This is the first research to utilize RAPD, ISSR and DAMD molecular markers to examine the extent of genetic variability among germplasm of gerbera.…”

“…This is not surprising as these markers are known to target different genomic fractions involving repeat and/or unique sequences, which may have been differentially evolved or preserved in due course of natural or human selection. These techniques were more informative and effective than the previously used molecular methods to study variation in gerbera, such as SSR (Hajibarat et al, 2014), and RAPD markers (Feghhi et al, 2014, Prajapati et al, 2014, Saidi et al, 2018. Discordance between dendograms obtained by ISSR and DAMD with RAPD could be explained by the different nature of each technique, region coverage of genome by each marker, level of polymorphism and the number of loci.…”

“…This technique utilized help effectively in genetic diversity analysis in gerbera. Genetic diversity in the gerbera has been reported using diverse molecular markers such as RAPD (Prajapati et al, 2014), and EST-SSR (Gong et al, 2010), ISSR (Li et al, 2004). In the present study, RAPD, ISSR and DAMD markers were used as useful tools for genetic variability analysis to provide more comprehensive insight regarding the genetic relationships and germplasm management of the cultivars utilized in this study (Kumar et al, 2011).…”

Phenotypic Variation in Flower Color and Morphology in the Gerbera (Gerbera hybrida) F1 Hybrid Population and Their Association with EST-SSR Markers

“…Molecular marker-assisted selection (MAS) can accelerate directional breeding by developing molecular markers that are linked to specific traits [ 15 , 16 ]. Previous studies have used RAPD [ 17 ], DAMD [ 18 ], ISSR [ 18 ], and SSR [ 19 , 20 , 21 ] markers to evaluate the genetic diversity of gerbera. However, few studies have focused on molecular markers related to important ornamental traits of gerbera.…”