Dissipation of carbendazim and its metabolites in cucumber using liquid chromatography tandem mass spectrometry

Mahdavi, Vahideh; Ghorbani-Paji, Fatemeh; Ramezani, Mohammad Kazem; Ghassempour, Alireza; Aboul‐Enein, Hassan Y.

doi:10.1080/03067319.2019.1617281

Cited by 18 publications

(8 citation statements)

References 21 publications

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“…The recovery experiment was carried out by adding a known concentration of the standard carbendazim into the cucumber juice from the cucumber sample. The spiked concentration of carbendazim was based on the information with the experimental evidences [ 11 ]. Table 2 shows the recovery of carbendazim in the cucumber samples was in the range of 96–104%.…”

Section: Resultsmentioning

confidence: 99%

“…The tubes were centrifuged at 4000 rpm for 5 min; solid residue was discarded and mixed with 2 mL of glacial acetic acid. It was treated by shaking in a vortex and centrifuging for 2 min at 4000 rpm [ 11 ]. The collected supernatants were used for the detection of carbendazim.…”

Section: Methodsmentioning

confidence: 99%

“…The main analytical methods for the detection of carbendazim include high-performance liquid chromatography (HPLC) [ 5 ], gas chromatography-mass spectrometry (GC-MS) [ 6 ], surface-enhanced Raman scattering (SERS) [ 7 ], colorimetric method [ 8 ], fluorescent method [ 9 ], and electrochemical sensor [ 10 ]. HPLC and GC-MS are classical analytical methods for the detection of pesticides in the laboratory, with high sensitivity and selectivity, but these often require one complex sample pretreatment [ 11 ]. SERS offers a high sensitivity, but its instability of detection signal results in poor accuracy and repeatability [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…HPLC and GC-MS are classical analytical methods for the detection of pesticides in the laboratory, with high sensitivity and selectivity, but these often Wang Jin and Li Ruiyi contributed equally to this work. require one complex sample pretreatment [11]. SERS offers a high sensitivity, but its instability of detection signal results in poor accuracy and repeatability [12].…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical detection of carbendazim with mulberry fruit-like gold nanocrystal/multiple graphene aerogel and DNA cycle amplification

Jin

Ruiyi

et al. 2021

Microchim Acta

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Graphical abstract An aptasensor for electrochemical detection of carbendazim is reported with mulberry fruit-like gold nanocrystal (MF-Au)/multiple graphene aerogel (MGA) and DNA cycle amplification. HAuCl 4 was reduced by ascorbic acid in a CTAC solution containing KBr and KI and formed trioctahedron gold nanocrystal. The gold nanocrystal underwent structural evolution under enantioselective direction of l -cysteine. The resulting MF-Au shows a mulberry fruit-like nanostructure composed of gold nanocrystals of about 200 nm as the core and many irregular gold nanoparticles of about 30 nm as the shell. The exposure of high-index facets improves the catalytic activity of MF-Au. MF-Au/MGA was used for the construction of an aptasensor for electrochemical detection of carbendazim. The aptamer hybridizes with assistant strand DNA to form duplex DNA. Carbendazim binds with the formed duplex DNA to release assistant strand DNA, triggering one three-cascade DNA cycle. The utilization of a DNA cycle allows one carbendazim molecule to bring many methylene blue–labeled DNA fragments to the electrode surface. This promotes significant signal amplification due to the redox reaction of methylene blue. The detection signal is further enhanced by the catalysis of MF-Au and MGA towards the redox of methylene blue. A differential pulse voltammetric signal, best measured at − 0.32 V vs. Ag/AgCl, increases linearly with the carbendazim concentration ranging from 1.0 × 10 −16 to 1.0 × 10 −11 M with a detection limit of 4.4 × 10 −17 M. The method provides ultrahigh sensitivity and selectivity and was successfully applied to the electrochemical detection of carbendazim in cucumber. This study reports on an ultrasensitive aptasensor for electrochemical detection of carbendazim in cucumber based on mulberry fruit–like gold nanocrystal–multiple graphene aerogel and DNA cycle double amplification. Supplementary Information The online version contains supplementary material available at 10.1007/s00604-021-04886-y.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrochemical detection of carbendazim with mulberry fruit-like gold nanocrystal/multiple graphene aerogel and DNA cycle amplification

Jin

Ruiyi

et al. 2021

Microchim Acta

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“…Aromatic hydroxylation of benzimidazole is one of the primary transformations during its transportation and delivery. 15 Such oxidation is also found in animals. The resulting phase I metabolite is further converted to the corresponding glucuronide and sulfate conjugate and then excreted in urine and bile.…”

Section: Introductionmentioning

confidence: 88%

Metabolic Activation and Cytotoxicity of Fungicide Carbendazim Mediated by CYP1A2

Shi

Zhao

et al. 2022

J. Agric. Food Chem.

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Carbendazim (CBZ) is a broad-spectrum fungicide widely used in many nations for foliar spray as well as seed and soil treatment. The resulting contamination and environmental pollution have been drawing public attention. In particular, CBZ was reported to cause liver damage in rats and zebrafish, and the mechanisms of its toxicity have not been clarified. The purposes of this study were to investigate the metabolic activation of CBZ and to determine a possible role of the reactive metabolites in CBZ-induced liver injury reported. One oxidative metabolite (M1), one glutathione conjugate (M2), and one N-acetyl cysteine conjugate (M3) were detected in human and rat liver microsomal incubations fortified with glutathione or N-acetyl cysteine after exposure to CBZ. CYP1A2 was the major enzyme responsible for the metabolic activation of CBZ. Biliary M2 and urinary M3 were detected in rats treated with CBZ. CBZ-derived protein adduction was found in cultured rat primary hepatocytes treated with CBZ. The increase of administration concentration intensified not only the cytotoxicity but also protein adduction induced by CBZ, suggesting a correlation of the cytotoxicity with the observed protein modification. The findings facilitate the understanding of the mechanisms of toxic action of CBZ.

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MWCNTs and ZnO-based Ce-MOF nanocomposites as enhanced sensing platform for electrochemical detection of carbendazim in Chinese traditional herbs samples

et al. 2023

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Dissipation of carbendazim and its metabolites in cucumber using liquid chromatography tandem mass spectrometry

Cited by 18 publications

References 21 publications

Electrochemical detection of carbendazim with mulberry fruit-like gold nanocrystal/multiple graphene aerogel and DNA cycle amplification

Electrochemical detection of carbendazim with mulberry fruit-like gold nanocrystal/multiple graphene aerogel and DNA cycle amplification

Metabolic Activation and Cytotoxicity of Fungicide Carbendazim Mediated by CYP1A2

MWCNTs and ZnO-based Ce-MOF nanocomposites as enhanced sensing platform for electrochemical detection of carbendazim in Chinese traditional herbs samples

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