“…In addition, cells were treated with ketoconazole (KTC, a selective inhibitor of CYP3A, 10 μM) or vehicle for 1 h before the medium was discarded. Then, the cells were mixed with CTU at 25, 50, 100, and 200 μM, respectively, and cultured for another 12 h. 25 Separately, cells were mixed with 2,6-dichloro-4-nitrophenol (DCNP, a selective inhibitor of sulfotransferase, 10 μM) and incubated for 12 h at 37 °C. The resulting medium was discarded, and cells were mixed with CTU at 150 μM and cultured for 12 h. After that, the cells were collected and ultrasonicated.…”