Electrochemical detection of carbendazim with mulberry fruit-like gold nanocrystal/multiple graphene aerogel and DNA cycle amplification

Abstract

Graphical abstract An aptasensor for electrochemical detection of carbendazim is reported with mulberry fruit-like gold nanocrystal (MF-Au)/multiple graphene aerogel (MGA) and DNA cycle amplification. HAuCl 4 was reduced by ascorbic acid in a CTAC solution containing KBr and KI and formed trioctahedron gold nanocrystal. The gold nanocrystal underwent structural evolution under enantioselective direction of l -cysteine. The resulting MF-Au shows a mulberry fruit-like nanostructure composed of gold nanocrystals of about 200 nm as the core and many irregular gold nanoparticles of about 30 nm as the shell. The exposure of high-index facets improves the catalytic activity of MF-Au. MF-Au/MGA was used for the construction of an aptasensor for electrochemical detection of carbendazim. The aptamer hybridizes with assistant strand DNA to form duplex DNA. Carbendazim binds with the formed duplex DNA to release assistant strand DNA, triggering one three-cascade DNA cycle. The utilization of a DNA cycle allows one carbendazim molecule to bring many methylene blue–labeled DNA fragments to the electrode surface. This promotes significant signal amplification due to the redox reaction of methylene blue. The detection signal is further enhanced by the catalysis of MF-Au and MGA towards the redox of methylene blue. A differential pulse voltammetric signal, best measured at − 0.32 V vs. Ag/AgCl, increases linearly with the carbendazim concentration ranging from 1.0 × 10 −16 to 1.0 × 10 −11 M with a detection limit of 4.4 × 10 −17 M. The method provides ultrahigh sensitivity and selectivity and was successfully applied to the electrochemical detection of carbendazim in cucumber. This study reports on an ultrasensitive aptasensor for electrochemical detection of carbendazim in cucumber based on mulberry fruit–like gold nanocrystal–multiple graphene aerogel and DNA cycle double amplification. Supplementary Information The online version contains supplementary material available at 10.1007/s00604-021-04886-y.

Trends on Aerogel-Based Biosensors for Medical Applications: An Overview

“…A high level of carbendazim in food has been associated with some side effects in humans, such as endocrine disorders and cancers. This way, an aptasensor for electrochemical detection of carbendazim was developed by Jin and co-workers [140] with gold nanocrystal/multiple graphene aerogel and DNA cycle amplification. The gold nanocrystal underwent structural evolution under enantioselective direction of L-cysteine.…”

Design and Fabrication of a Biomimetic Smart Material for Electrochemical Detection of Carbendazim Pesticides in Real Samples with Enhanced Selectivity

“…MIPs are ideal for use as sorbents in the detection of trace levels of pesticides because of their many intrinsic benefits, including their high selectivity, sensitivity [24], reusability, physicochemical stability [25], and cost-effectiveness [26]. In recent years, there has been a dramatic increase in the fascination of utilizing molecularly imprinted polymers The detection of CBZ has been investigated using a wide range of analytical methods including high-performance liquid chromatography (HPLC) [12,13], gas chromatography (GC) [5,9], liquid chromatography (LC) coupled with mass spectrometry (LC/MS-MS) [14,15], electrochemical techniques [16][17][18], and fluorescent sensors [19]. Additionally, various sample preparation procedures have been employed.…”

“…The detection of CBZ has been investigated using a wide range of analytical methods including high-performance liquid chromatography (HPLC) [ 12 , 13 ], gas chromatography (GC) [ 5 , 9 ], liquid chromatography (LC) coupled with mass spectrometry (LC/MS–MS) [ 14 , 15 ], electrochemical techniques [ 16 , 17 , 18 ], and fluorescent sensors [ 19 ]. Additionally, various sample preparation procedures have been employed.…”

Graphitic Carbon Nitride Incorporated Europium Molybdate Composite as an Enhanced Sensing Platform for Electrochemical Detection of Carbendazim in Agricultural Products

“…In particular, fungicides play the main role in the high productivity attained in agriculture via the control of fungi and insects. [1][2][3] Additionally, a fungus can seriously harm agriculture, leading to significant losses in output, profit, and quality. Fungicides are chemical substances that are applied to agricultural grounds to prevent and kill fungus spores.…”