Dissemination of veterinary antibiotics and corresponding resistance genes from a concentrated swine feedlot along the waste treatment paths

Abstract

Swine feedlots are an important pollution source of antibiotics and antibiotic resistance genes (ARGs) to the environment. This study investigated the dissemination of two classes of commonly-used veterinary antibiotics, namely, tetracyclines (TCs) and sulfonamides (SAs), and their corresponding ARGs along the waste treatment paths from a concentrated swine feedlot located in Beijing, China. The highest total TC and total SA concentrations detected were 166.7 mg kg −1 and 64.5 μg kg −1 in swine manure as well as 388.7 and 7.56 μg L −1 in swine wastewater, respectively. Fourteen tetracycline resistance genes (TRGs) encoding ribosomal protection proteins (RPP), efflux proteins (EFP) and enzymatic inactivation proteins, three sulfonamide resistance genes (SRGs), and two integrase genes were detected along the waste treatment paths with detection frequencies of 33.3-75.0%. The relative abundances of target ARGs ranged from 2.74 × 10 -6 to 1.19. The antibiotics and ARGs generally declined along both waste treatment paths, but their degree of reduction was more significant along the manure treatment path. The RPP TRGs dominated in the upstream samples and then decreased continuously along both waste treatment paths, whilst the EFP TRGs and SRGs maintained relatively stable. Strong correlations between antibiotic concentrations and ARGs were observed among both manure and wastewater samples. In addition, seasonal temperature, and integrase genes, moisture content and nutrient level of tested samples could all impact the relative abundances of ARGs along the swine waste treatment paths. This study helps understand the evolution and spread of ARGs from swine feedlots to the environment as well as assess the environmental risk arising from swine waste treatment.

Systematic analysis of occurrence, density and ecological risks of 45 veterinary antibiotics: Focused on family livestock farms in Erhai Lake basin, Yunnan, China

“…The results are consistent with many other studies. For example, Wang et al. (2016) indicated that the total TCs concentrations could be high to 166.7 mg/kg in pig manure and 388.7 μg/L in wastewater.…”

Isolation, Screening, and Characterization of Antibiotic-Degrading Bacteria for Penicillin V Potassium (PVK) from Soil on a Pig Farm

“…The use of antibiotics in livestock production is forbidden in some regions in the world, (e.g., the European Union since 2006) because of the overuse, and misuse or lack of control in administering antibiotics, resulting in the accumulation of antibiotics in animal products and the environment [4,5]. Research has shown that the spread of multidrug resistant bacterial strains and their potential adverse effects on animals and environment are closely associated with the abuse of antibiotics [6,7]. Numerous studies have reported the detection of antibiotics in coastal water and wastewater at concentrations from μg/L to mg/L levels [8].…”

Compostagem de efluente suíno no tratamento de resíduos de fármacos veterinários

“…A mixed one-way analysis of covariance (ANCOVA. They also potentialize the microorganism resistance to antibiotics (BONDARCZUK et al, 2016;CENTNER, 2016;LUCAS et al, 2016;RHOUMA et al, 2016;WANG et al, 2016a;HU et al, 2018) namely, tetracyclines (TCs, endangering the human health REGITANO;LEAL, 2010).…”