Isolation, Screening, and Characterization of Antibiotic-Degrading Bacteria for Penicillin V Potassium (PVK) from Soil on a Pig Farm

Yang, Xin; Li, Miao; Guo, Panpan; Li, Hualong; Hu, Zhengyan; Liu, Xianwang; Zhang, Qiang

doi:10.3390/ijerph16122166

Cited by 23 publications

(9 citation statements)

References 21 publications

(20 reference statements)

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“…In addition, whole bacterial cells can be used for ␤-lactam antibiotic degradation. The ␤-lactam-degrading bacteria most commonly reported in the literature are mainly Bacillus species, such as Bacillus cereus (11,24). To the best of our knowledge, this study is the first report of whole-cell biodegradation of PVK by an Ochrobactrum species.…”

Section: Discussionmentioning

confidence: 80%

“…However, with the widespread use of PVK, substantial concentrations of PVK have been found in livestock manure, soil, and wastewater effluents, which may pose potential threats to human health and contribute to the emergence of penicillinresistant bacterial strains. Therefore, it is imperative for researchers to identify ways to degrade PVK residues in the environment (11).…”

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confidence: 99%

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Isolation and Characterization of Ochrobactrum tritici for Penicillin V Potassium Degradation

Wang

Shen

et al. 2020

mSphere

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Substantial concentrations of penicillin V potassium (PVK) have been found in livestock manure, soil, and wastewater effluents, which may pose potential threats to human health and contribute to the emergence of penicillin-resistant bacterial strains. In this study, bacterial strains capable of degrading PVK were isolated from sludge and characterized. Strain X-2 was selected for biodegradation of PVK. Based on morphological observations and 16S rRNA gene sequencing, strain X-2 was identified as an Ochrobactrum tritici strain. To enhance the PVK degradation ability of PVK, a whole-cell biodegradation process of Ochrobactrum tritici X-2 was established and optimized. In the whole-cell biodegradation process, the optimal temperature and pH were 30°C and 7.0, respectively. Under the optimized conditions, the degradation rate using 0.5 mg/ml PVK reached 100% within 3 h. During biodegradation, two major metabolites were detected: penicilloic acid and phenolic acid. The present study provides a novel method for the biodegradation of PVK using Ochrobactrum tritici strains, which represent promising candidates for the industrial biodegradation of PVK. IMPORTANCE Substantial concentrations of penicillin V potassium (PVK) have been found in the environment, which may pose potential threats to human health and contribute to the emergence of penicillin-resistant bacterial strains. In this study, antibiotic-degrading bacterial strains for PVK were isolated from sludge and characterized. Ochrobactrum tritici was selected for the biodegradation of PVK with high efficiency. To enhance its PVK degradation ability, a whole-cell biodegradation process was established and optimized using Ochrobactrum tritici. The degradation rate with 0.5 mg/ml PVK reached 100% within 3 h. The potential biodegradation pathway was also investigated. To the best of our knowledge, the present study provides new insights into the biodegradation of PVK using an Ochrobactrum tritici strain, a promising candidate strain for the industrial biodegradation of β-lactam antibiotics.

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Section: Discussionmentioning

confidence: 80%

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confidence: 99%

Isolation and Characterization of Ochrobactrum tritici for Penicillin V Potassium Degradation

Wang

Shen

et al. 2020

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“…Isolating and screening AMX-degrading bacterial strains were performed in three steps, namely enrichment, acclimatization and separation. The details of the steps can be found in the previously published reference [17].…”

Section: Isolation and Selection Of Amx-degrading Bacterial Strainsmentioning

confidence: 99%

Optimization of Culture Conditions for Amoxicillin Degrading Bacteria Screened from Pig Manure

Yang

Guo

et al. 2020

IJERPH

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(1) Objective: The objective of this study was to screen amoxicillin (AMX)-degrading bacterial strains in pig manure and optimize the fermentation conditions for these strains to achieve high fermentation rate, which can provide an effective way for the practical application of bacterial strains as antibiotic-degrading bacterial in treating livestock waste for antibiotic residues. (2) Methods: Antibiotic susceptibility tests and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) were employed to screen AMX-degrading bacterial strains in pig manure. The culture conditions were optimized for AMX-degrading bacterial strains using Plackeet–Burman design (PBD), the steepest ascent design, and the response surface methods, coupled with the Box–Behnken design (BBD). The effects of culture time, temperature, rotator (mixing) speed, inoculum level, and initial pH value on the growth of AMX-degrading strains were investigated. Experimental data obtained from BBD were utilized to generate a second-order polynomial regression model for evaluating the effects of the tested variables on the optical density at 600 nm (OD600) of culture solutions as the growth indicator for the screened AMX-degrading strains. (3) Results: The initial pH, culture time, and the inoculum level had significant effects on the OD600 value (growth) of the screened AMX-degrading strains. The initial pH value was found to be the most critical factor influencing the growth of bacteria. The optimized culture condition for the bacterial growth determined by the response surface methodology was: the initial pH of 6.9, culture time of 52 h, and inoculum level of 2%. The average OD value of 12 different fermentation conditions in the initial fermentation tests in this study was 1.72 and the optimization resulted in an OD value of 3.00. The verification experiment resulted in an OD value of 2.94, which confirmed the adequacy of the optimization model for the determining the optimal culture condition. (4) Conclusions: The growth of the screened strain of AMX-degrading bacteria could be optimized by changing the fermentation conditions. The optimization could be achieved by using the Box–Behnken response surface method and Plackett–Burman experimental design.

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“…With respect to biodegradation mechanisms, most β-lactam antibiotic degradation studies have focused on the separation and characterization of key intermediate metabolites and not the whole pathway, including the mineralization products [21][22][23][24]. HPLC-mass spectrometry has typically been used as the primary detection method; however, such a tool cannot absolutely con rm the structure of degradation products or fully clarify the changes in the degradation processes [25].…”

Section: Introductionmentioning

confidence: 99%

Degradation of Penicillin G by Visible Light Irradiation-assisted Biotransformation by Whole Cell Paracoccus Sp.

Wang

Shen

Cong

et al. 2021

Preprint

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Background: The Paracoccus sp. strain (KDSPL-02) isolated from sludge was identified and evaluated for catalytic activity in the degradation of penicillin G. Results: High degradation efficiency and synergistic catalytic effects of the whole cell and visible light without additional catalysts were observed. The key factors influencing the degradation and kinetics of penicillin G were investigated. The results showed the phenylacetic acid, which was produced during penicillin G biodegradation, exhibited stronger inhibiting effects on KDSPL-02. However, this effect was reduced by visible light irradiation without any additional photocatalyst; furthermore, the rate of penicillin G biodegradation was accelerated, reaching a 100% rate in 12 h at a penicillin G concentration of 1.2 g/L. Four key intermediates produced during penicillin G degradation were isolated and identified by LC–MS, 1H NMR, and 13C NMR. Enzymes involved in the PAA pathway were proposed from a genomic analysis of paracoccus sp. KDSPL-02. Conclusions:These results provide a new method for bio-degrading of penicillin or other antibiotic pollutants using photoaccelerating biocatalysts with greater efficiency and more environmentally friendly conditions.

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Isolation, Screening, and Characterization of Antibiotic-Degrading Bacteria for Penicillin V Potassium (PVK) from Soil on a Pig Farm

Cited by 23 publications

References 21 publications

Isolation and Characterization of Ochrobactrum tritici for Penicillin V Potassium Degradation

Isolation and Characterization of Ochrobactrum tritici for Penicillin V Potassium Degradation

Optimization of Culture Conditions for Amoxicillin Degrading Bacteria Screened from Pig Manure

Degradation of Penicillin G by Visible Light Irradiation-assisted Biotransformation by Whole Cell Paracoccus Sp.

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