Optimization of Culture Conditions for Amoxicillin Degrading Bacteria Screened from Pig Manure

Yang, Xin; Guo, Panpan; Li, Miao; Li, Hualong; Hu, Zhengyan; Liu, Xianwang; Zhang, Qiang

doi:10.3390/ijerph17061973

Cited by 23 publications

(16 citation statements)

References 30 publications

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“…Therefore, these 3 factors were optimized using D-optimal experimental design in RSM. RSM is an efficient technique that can determine the best fermentation conditions for a multi-variable system mathematically and statistically [ 41 ]. It is favorable over classical optimization methods because it is fast, reliable, helps understand the effect of varying concentrations of nutrients and leads to a substantial reduction in total number of runs therefore saving time, chemicals and manpower [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Production and statistical optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in solid state fermentation

et al. 2021

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Background Paromomycin is a 2-deoxystreptamine aminocyclitol aminoglycoside antibiotic with broad spectrum activity against Gram-negative, Gram-positive bacteria and many protozoa. This study introduces a strategy for paromomycin production through solid-state fermentation using Streptomyces rimosus subsp. paromomycinus NRRL 2455. Solid state fermentation has gained enormous attention in the development of several products because of their numerous advantages over submerged liquid fermentation. After selecting the best solid substrate, a time course study of paromomycin production was carried out followed by optimization of environmental conditions using response surface methodology. Paromomycin yields obtained using this technique were also compared to those obtained using submerged liquid fermentation. Results Upon screening of 6 different substrates, maximum paromomycin concentration (0.51 mg/g initial dry solids) was obtained with the cost-effective agro-industrial byproduct, corn bran, impregnated with aminoglycoside production media. Optimization of environmental conditions using D-optimal design yielded a 4.3-fold enhancement in paromomycin concentration reaching 2.21 mg/g initial dry solids at a pH of 8.5, inoculum size of 5% v/w and a temperature of 30 °C. Conclusion Compared to submerged liquid fermentation, solid state fermentation resulted in comparable paromomycin concentrations, cost reduction of raw materials, less energy consumption and waste water discharge, which have major implications in industrial fermentation. Therefore, solid state fermentation is a promising alternative to submerged liquid fermentation for paromomycin production. To the best of our knowledge, this is the first report on the optimized paromomycin production through solid state fermentation process.

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Section: Discussionmentioning

confidence: 99%

Production and statistical optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in solid state fermentation

et al. 2021

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“…El-Naggar et al (2013) also described that Gram-positive and Gram-negative bacteria can be effectively inhibited by prevalent antibiotics; furthermore, the antibiotic resistance was greatly due to the application of multiple drugs available worldwide [ 3 ]. Furthermore, Anjum et al (1997) and Liu et al (1999) suggested that BBD is an efficient tool for determining the RSM for checking the optimization of various factors and conditions [ 14 , 18 ].…”

Section: Resultsmentioning

confidence: 99%

“…Further, results could be analyzed through the Box–Behnken method which is an extension of factorial design. This experimental design is arranged in such a way that each point is localized in the center and middle of the edges of a cube and increases the design points as the number of polynomial coefficients [ 14 ]. The main disadvantage of chemometry is that it could not undergo the experiment in an extreme condition where the chances of unsatisfactory results are very high [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation and Optimization of Antibiotics Resistance Profile against Clostridium perfringens from Buffalo and Cattle in Pakistan

et al. 2021

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Clostridium perfringens is a serious threat to successful bovine farming. It causes severe damage to the buffalo and cattle health causing a drastic reduction in milk and meat production. In Pakistan, C. perfringens is a constant threat, and for its management, antibiotics are mostly used. Most bovine farmers use a single antibiotic to suppress the bacterial infection which in turn, increases the antimicrobial resistance (AMR) against the particular antibiotic. To reduce the resistance, the administration of multiple antibiotics in their standard doses at different times can be a possible remedy to manage the AMR and reduce their viability. This study aims to evaluate the effect of 11 commonly used antibiotics at their standard concentrations for inhibiting 33 strains of C. perfringens from five districts of Punjab province in Pakistan. Based on the zone of inhibition, ciprofloxacin, ampicillin, and cefotaxime (CAC) at their standard concentrations effectively inhibited the bacterium. These antibiotics showed appropriate significance statistically, i.e., correlation, Chi-square test, and cluster analysis. Optimization of these antibiotics using response surface methodology (RSM) revealed that the selected antibiotics from medium to high range not only reduce the bacterial propagation but also their population up to a considerable extent. Hence, the health of milk- and meat-producing large animals could be improved, which will be cost-effective and less harmful to the animal, human health, and the environment. Moreover, optimized administration of the selected antibiotics would reduce the impact of drug-resistant superbugs.

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“…were optimized using D-optimal experimental design in RSM. RSM is an e cient technique that can determine the best fermentation conditions for a multi-variable system mathematically and statistically [48]. It is favorable over classical optimization methods because it is fast, reliable, helps understand the effect of varying concentrations of nutrients and leads to a substantial reduction in total number of runs therefore saving time, chemicals and manpower [49].…”

Section: Discussionmentioning

confidence: 99%

Production and Statistical Optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in Solid State Fermentation

El-Housseiny

Ibrahim

Yassien

et al. 2020

Preprint

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Background: Paromomycin is a 2 deoxystreptamine aminocyclitol aminoglycoside antibiotic with broad spectrum activity against Gram-negative, Gram-positive bacteria and many protozoa. This study introduces a strategy for paromomycin production through solid-state fermentation using Streptomyces rimosus subsp. paromomycinus NRRL 2455. Solid state fermentation has gained enormous attention in the development of several products because of their numerous advantages over submerged liquid fermentation. After selecting the best solid substrate, a time course study of paromomycin production was carried out followed by optimization of environmental conditions using response surface methodology. Paromomycin yields obtained using this technique were also compared to those obtained using submerged liquid fermentation.Results: Upon screening of 6 different substrates, maximum paromomycin concentration (0.51 mg/g initial dry solids) was obtained with the cost-effective agro-industrial byproduct, corn bran, impregnated with aminoglycoside production media. This value was higher than that obtained using submerged liquid fermentation using the same conditions. Optimization of environmental conditions using D optimal design yielded a 4.3-fold enhancement in paromomycin concentration reaching 2.21 mg/g initial dry solids at a pH of 8.5, inoculum size of 5% v/w and a temperature of 30 °C. Conclusion: Compared to submerged liquid fermentation, solid state fermentation resulted in higher paromomycin concentrations, cost reduction of raw materials, less energy consumption and waste water discharge, which have major implications in industrial fermentation. Therefore, SSF is a superior alternative to SLF for paromomycin production. To the best of our knowledge, this is the first report on the optimized paromomycin production through solid state fermentation process.

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Optimization of Culture Conditions for Amoxicillin Degrading Bacteria Screened from Pig Manure

Cited by 23 publications

References 30 publications

Production and statistical optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in solid state fermentation

Production and statistical optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in solid state fermentation

Evaluation and Optimization of Antibiotics Resistance Profile against Clostridium perfringens from Buffalo and Cattle in Pakistan

Production and Statistical Optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in Solid State Fermentation

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