Production and statistical optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in solid state fermentation

Abstract

Background Paromomycin is a 2-deoxystreptamine aminocyclitol aminoglycoside antibiotic with broad spectrum activity against Gram-negative, Gram-positive bacteria and many protozoa. This study introduces a strategy for paromomycin production through solid-state fermentation using Streptomyces rimosus subsp. paromomycinus NRRL 2455. Solid state fermentation has gained enormous attention in the development of several products because of their numerous advantages over submerged liquid fermentation. After selecting the best solid substrate, a time course study of paromomycin production was carried out followed by optimization of environmental conditions using response surface methodology. Paromomycin yields obtained using this technique were also compared to those obtained using submerged liquid fermentation. Results Upon screening of 6 different substrates, maximum paromomycin concentration (0.51 mg/g initial dry solids) was obtained with the cost-effective agro-industrial byproduct, corn bran, impregnated with aminoglycoside production media. Optimization of environmental conditions using D-optimal design yielded a 4.3-fold enhancement in paromomycin concentration reaching 2.21 mg/g initial dry solids at a pH of 8.5, inoculum size of 5% v/w and a temperature of 30 °C. Conclusion Compared to submerged liquid fermentation, solid state fermentation resulted in comparable paromomycin concentrations, cost reduction of raw materials, less energy consumption and waste water discharge, which have major implications in industrial fermentation. Therefore, solid state fermentation is a promising alternative to submerged liquid fermentation for paromomycin production. To the best of our knowledge, this is the first report on the optimized paromomycin production through solid state fermentation process.

A biostatistical approach for augmenting rare bianthraquinone antibiotic production by Streptomyces sp. RA-WS2 using Taguchi design

“…The contour plots explaining the relation between the process parameters and biomass as well as setomimycin production. Such relationships were found to have similar trend with that of reported for other antibiotics (Wang et al, 2008 ; El-Housseiny et al, 2021 ). After determining the optimal parameters, Taguchi model suggested solution for optimum production of DCW as well as setomimycin production followed by validation experiments.…”

Evaluation of fortimicin antibiotic combinations against MDR Pseudomonas aeruginosa and resistome analysis of a whole genome sequenced pan-drug resistant isolate

“…The results mostly showed synergistic effect on some selected clinically important MDR pathogens [ 33 ]. The tested paromomycin was naturally produced by Streptomyces rimosus NRRL 2455 after several rounds of statistical and physiological optimizations [ 34 ].…”

Metagenomic nanopore sequencing for exploring the nature of antimicrobial metabolites of Bacillus haynesii

“…One of the promising approaches to optimize the production of certain microbial metabolites is via the use of various models implemented for statistical optimization. This approach has been successfully used for the production optimization of various secondary antibacterial metabolites such as paromomycin (Ibrahim et al 2019 ; El-Housseiny et al 2021 ; Ibrahim et al 2023 , antifungal metabolites (El-Sayed et al 2020 ), biosurfactants (El-Housseiny et al 2016 , 2020 ), probiotics against life-threatening pathogens (Mansour et al 2018 ), medically-used enzymes such as L-asparaginase (Darnal et al 2023 ), enterokinase, (Ebrahimifard et al 2022 ), staphylokinase (Shariati et al 2022 ), and carbohydrases (Kaur et al 2021 )using the response surface methodology and multifactorial design. Therefore, this approach could be a promising tool for the optimization procedures of the potential antibacterial metabolite produced by B. haynesii MZ922052.…”