Direct plant regeneration from mature leaf explants of pistachio, Pistacia vera L.

Plant Tissue Culture: Current Status and Opportunities

“…The surface sterilization of explant in chemical solutions is an important step to remove contaminants with minimal damage to plant cells [17]. The most commonly used disinfectants are sodium hypochlorite [18,19], calcium hypochlorite [20], ethanol [21] and mercuric chloride (HgCl2) [17]. The cultures are incubated in growth chamber either under light or dark conditions according to the method of propagation.…”

In vitro Plantlets Regeneration from Nodal Segments of Murici (Byrsonima gardneriana)

“…However, for many plant species, the use of IBA was shown to be beneficial, such as with A. cearenses specie, presenting rooting rate of 92% in the presence of 10.0 µM of IBA (Campos, Lima-Brito, Gutierrez, Santana, & Souza, 2013). V. agnus-castus presented 90% (Balaraju, Lima-Brito, Gutierrez, Santana, & Souza, 2008) and Pistaceae vera L. presenting 84% (Tilkat, Onay, Yıldırım, & Ayaz, 2009) of rooting, both species in culture medium supplemented with 0.49 µM and 9.84 µM de IBA, respectively. The IBA is the synthetic auxin most commonly used for inducing rooting for presenting the property of promoting the formation of early roots.…”

An efficient in vitro shoot regeneration from leaf petiolar explants and ex vitro rooting of Bixa orellana L.- A dye yielding plant

“…Adventitious shoot regeneration using leaf explants is established for several woody plant species, such as Paulownia tomentosa (Corredoira et al 2008), Prunus serotina (Liu and Pijut 2008) Citrus sinensis (Khan et al 2009), Pistacia vera (Tilkat et al 2009), blackberry (Sandhya and Mahalaxmi 2009), Morus alba (Kodandaram et al 2013). Similar to earlier observations, kinetin did not promote shoot multiplication in any of the combinations tested in the present investigation (Madhuri and D'Souza, 2000).…”