An efficient in vitro shoot regeneration from leaf petiolar explants and ex vitro rooting of Bixa orellana L.- A dye yielding plant

Mohammed, Arifullah; Chiruvella, Kishore K.; Namsa, Nima D.; Ghanta, Rama Gopal

doi:10.1007/s12298-015-0297-z

Cited by 15 publications

(6 citation statements)

References 31 publications

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“…Annatto is a species in which pluripotency of cells and tissues has been proven from various types of explants derived from seedlings, such as shoot and nodal segments (D'Souza and Sharon 2001), hypocotyl segments (Paiva Parimalan et al 2007), inverted rooted hypocotyls (Paiva Neto et al 2003/4), cotyledonary nodes (Carvalho et al 2005), cotyledons (Parimalan et al 2007), root segments , petiole segments (Mohammed et al 2015), and from nodal and internodal adult stem segments (Siril and Joseph 2013; Cruz et al 2015). Additionally, several plant growth regulators were tested on these explants, such as auxins, i.e., indole-3-acetic acid (IAA), 2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthalene acetic acid (NAA), and indole-3-butyric acid (IBA) alone or, most commonly, in combination with cytokinins like 6-γ,γ-dimetylalylamino-purine (2-iP), zeatin (ZEA), kinetin (Kin), 6-benzyladenine (BA), and thidiazuron (TDZ)…”

Section: Introductionmentioning

confidence: 99%

Somatic Embryogenesis: Fundamental Aspects and Applications

Ochoa-Alejo¹,

Loyola‐Vargas²

2016

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Section: Introductionmentioning

confidence: 99%

Somatic Embryogenesis: Fundamental Aspects and Applications

Ochoa-Alejo¹,

Loyola‐Vargas²

2016

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“…Additionally, leaves as explants are more susceptible to contamination due to their larger surface area, making disinfection more challenging. Furthermore, direct and indirect shoot organogenesis from petiole explants has been documented in various plants 7,8 . Multiple Heuchera cultivars in this study successfully regenerated shoots from petiole culture, confirming petioles as excellent explants for Heuchera in vitro culture.…”

Section: Discussionmentioning

confidence: 99%

Development and comparative analysis of initiation ability in large-scale Heuchera propagation using tissue culture versus cuttings

Xu,

Guo,

Wang

et al. 2023

Sci Rep

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The Heuchera genus, a member of the Saxifragaceae family, encompasses a wide array of varieties and hybrids, serving both traditional medicinal and ornamental purposes. However, a significant knowledge gap persists in achieving efficient mass propagation of diverse Heuchera cultivars creating a substantial market void. To address this, our study focuses on expedited seedling regeneration by investigating leaf cutting and tissue culture techniques to offer novel insights to cultivators. Herein, we successfully rooted thirteen distinct cultivars from the Heuchera and Heucherella (Heuchera × Tiarella) genera through cutting. Moreover, in vitro culture experiments led to the successful induction of calli and shoots from petiole samples. Notably, variations in measured parameters were observed across cultivars in both cutting and tissue culture methodologies. When petiole explants were exposed to cytokinin 6-benzylaminopurine (BA) at concentrations of 0.5, 1.0, and 2.0 mg/L along with auxin α-naphthaleneacetic acid (NAA) at 0.5 mg/L, shoots were produced either directly or indirectly during the primary culture. Exposure to darkness and the application of 2,4-dichlorophenoxyacetic acid (2,4-D) did not promote shoot formation but were beneficial for callus stimulation. Interestingly, a negative correlation was observed between the ease of initiating cutting recovery and inducting tissue culture regeneration, suggesting that cultivars that easily regenerate through cutting might encounter difficulties during induction by tissue culture. In light of these findings, we devised a streamlined and effective protocol for rapid Heuchera propagation. This protocol involves micropropagation, directly acquiring adventitious shoots from primary cultures supplemented by cutting-based propagation methods.

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“…However, note that the shoot explants were whole plant destructive led to mother plant loss, coupled with petioles regenerating more shoots than leaves [5]. Moreover, the direct and indirect shoot organogenesis from petiole explants has been reported in plants [6,7]. The petiole could be an excellent explant source for Heuchera in vitro culture.…”

Section: Discussionmentioning

confidence: 99%

In vitro and cutting propagation of Heuchera: rapid mass production protocol and correlation between initiating ability the two methods

Guo

Wang

et al. 2022

Preprint

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There is still a lack of understanding about the efficient mass propagation of multiple Heuchera cultivars leading to a large market gap. And there has been no empirical research on the relationship between propagation methods of cutting and in vitro. In this study, thirteen different cultivars from the genus Heuchera and Heucherella (Heuchera × Tiarella) were successfully rooted by cutting, and calluses and shoots were also successfully inducted from petiole in vitro culture. However, measured indexes varied significantly among cultivars for cutting and tissue culture. Petiole explants treated with cytokinin 6-benzylaminopurine (BA: 0.5 ~ 2.0 mg/L) plus auxin α-naphthaleneacetic acid (NAA: 0.5 mg/L) formed shoots either directly or indirectly during primary culture. And the dark treatment and 2,4-dichlorophenoxyacetic acid (2,4-D) were ineffective for shoot formation but beneficial for callus stimulation. Furthermore, we discovered a negative correlation between initiating cutting recovery and tissue culture inducting, implying that cultivars with low difficulty in cutting initiating were relatively difficult to tissue culture. Then, we developed a simple and efficient Heuchera rapid propagation protocol. That is, micropropagation through obtaining adventitious shoots directly from primary cultures, supplemented by cutting propagation. Our petiole explants system and leaf-cutting technique are expected to guide Heuchera's mass production.

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An efficient in vitro shoot regeneration from leaf petiolar explants and ex vitro rooting of Bixa orellana L.- A dye yielding plant

Cited by 15 publications

References 31 publications

Somatic Embryogenesis: Fundamental Aspects and Applications

Somatic Embryogenesis: Fundamental Aspects and Applications

Development and comparative analysis of initiation ability in large-scale Heuchera propagation using tissue culture versus cuttings

In vitro and cutting propagation of Heuchera: rapid mass production protocol and correlation between initiating ability the two methods

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