In vitro Plantlets Regeneration from Nodal Segments of Murici (Byrsonima gardneriana)

Sá, Francisca S.; Porto, Jorge Marcelo Padovani; Brito, Alone Lima; Santana, José Raniere Ferreira de; Souza, Rafaeli Aparecida Vieira de; Braga, Francyane Tavares

doi:10.5539/jas.v10n4p402

JAS

2018

DOI: 10.5539/jas.v10n4p402

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In vitro Plantlets Regeneration from Nodal Segments of Murici (Byrsonima gardneriana)

Francisca S. Sá¹,

Jorge Marcelo Padovani Porto²,

Alone Lima Brito³

et al.

Abstract: This study aimed to develop efficient protocols for the in vitro micropropagation of Byrsonima gardneriana. ). The use of concentrations from 2.0 to 4.0 µM 6-benzylaminopurine was efficient in the multiplication of B. gardneriana, given that, using concentrations above these, a decrease in this efficiency occurs. The use of auxin interfered negatively with the results. In vitro rooting occurs even in medium free of auxin. The activated charcoal was insufficient for rooting. The use of growth regulators 6-benzy… Show more

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2023

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In Vitro Callogenesis of Murici-Pitanga (Byrsonima gardneriana a. Juss.) Malpighiaceae

Santos,

Roque,

Das Neves

et al. 2023

RGSA

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Objective: The present work aimed to evaluate the callogenesis of murici-pitanga in different explants and growth regulators. since this species has great medicinal, environmental and economic importance, however the cultivation process and extractivism make its propagation difficult. Therefore, in vitro cultivation provides conditions that can optimize its propagation. Among the techniques used in in vitro micropropagation, callogenesis allows the regeneration of tissues, organs or plants, becoming a cultivation alternative for the species under study. Method: Explants from seedlings cultivated in vitro were inoculated in MS 1/2 medium with different types, concentrations and combinations of growth regulators, namely: zygotic embryos under 6-benzylaminopurine x α-naphthaleneacetic acid (BAP x ANA) (0.0 ; 11.1, 22.2 and 44.4 µM); leaf and stem segments under NAA (0.0; 13.42; 26.85 and 40.27 µM) and root and leaf segments under 2,4-dichlorophenoxyacetic acid (2,4-D) (0.0; 2. 26; 4.52; 9.05 and 18.10 µM), with subcultures in cell suspensions (0.0; 4.52; 9.05; 18.10 and 3620 µM) and callus growth curve (4. 52 µM). Resultsandconclusion: The percentage of induction and mass of fresh callus matter were evaluated. The callogenesis of murici-pitanga occurs more efficiently in the root segment in a medium supplemented with 4.52 µM of 2,4-D. Where, the calluses presented friable characteristics, in addition to better averages of induction percentage and fresh matter mass. For cell suspension, a concentration of 4.52 µM of 2,4-D is indicated, and in the growth curve, the best period for callus subculture may occur on the 56th day of cultivation, established based on the callus growth pattern sigmoidal with three distinct phases. Therefore, the murici-pitanga can be regenerated through calluses originating from root segments. Originality/value: Studies that address the in vitro propagation of murici-pitanga are scarce, and the present work reports significant results, with new explant options and different types of growth regulators to be used. In addition to advances in studies of callus subcultures with great potential for regeneration. These results become important, since the species needs techniques that facilitate its propagation, and could support future morphogenetic studies, obtaining secondary metabolites, in addition to establishing a regeneration protocol via somatic embryogenesis.

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In Vitro Callogenesis of Murici-Pitanga (Byrsonima gardneriana a. Juss.) Malpighiaceae

Santos,

Roque,

Das Neves

et al. 2023

RGSA

View full text Add to dashboard Cite

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In vitro Plantlets Regeneration from Nodal Segments of Murici (Byrsonima gardneriana)

Cited by 1 publication

References 7 publications

In Vitro Callogenesis of Murici-Pitanga (Byrsonima gardneriana a. Juss.) Malpighiaceae

In Vitro Callogenesis of Murici-Pitanga (Byrsonima gardneriana a. Juss.) Malpighiaceae

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