Direct electrochemistry of hemoglobin on an ionic liquid carbon electrode modified with zinc tungstate nanorods

Ruan, Chengxiang; Sun, Zhaolan; Liu, Jun; Jin, Lihua; Gao, Weimin; Sun, Wei; Yang, Xiao

doi:10.1007/s00604-012-0796-3

Cited by 13 publications

(3 citation statements)

References 36 publications

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“…The app M K value was calculated as 2.062 mmol L -1 , which was smaller than that of some previous reported values such as GR-TiO2-Hb film (3.3 mmol L -1 ), [32] Nafion/ZnWO4-Hb/CILE (5.76 mmol L -1 ) [33] and CTS/GR-LDH-Hb/CILE (8.48 mmol L -1 ). [34] The lower value of app M K indicated that HRP immobilized with NiO nanoflower retained its bioactivity and had a high biological affinity to TCA.…”

Section: Electrochemical Investigationscontrasting

confidence: 69%

Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic Activity

Liang¹,

Li²,

Wang³

et al. 2016

Croat. Chem. Acta

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Abstract:In this paper nickel oxide (NiO) nanoflower was synthesized and used for the realization of direct electrochemistry of horseradish peroxidase (HRP). By using carbon ionic liquid electrode (CILE) as the substrate electrode, NiO-HRP composite was casted on the surface of CILE with chitosan (CTS) as the film forming material and the modified electrode was denoted as CTS/NiO-HRP/CILE. UV-Vis absorption and FT-IR spectra confirmed that HRP retained its native structure after mixed with NiO nanoflower. Direct electron transfer of HRP on the modified electrode was investigated by cyclic voltammetry with a pair of quasi-reversible redox waves appeared, indicating that the presence of NiO nanoflower on the electrode surface could accelerate the electron transfer rate between the electroactive center of HRP and the substrate electrode. Electrochemical behaviors of HRP on the modified electrode were carefully investigated. The HRP modified electrode showed excellent electrocatalytic activity to the reduction of trichloroacetic acid with wider linear range and lower detection limit. Therefore the presence of NiO nanoflower could provide a friendly biocompatible interface for immobilizing biomolecules and keeping their native structure. The fabricated electrochemical biosensor displayed the advantages such as high sensitivity, good reproducibility and long-term stability.

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Section: Electrochemical Investigationscontrasting

confidence: 69%

Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic Activity

Liang¹,

Li²,

Wang³

et al. 2016

Croat. Chem. Acta

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“…So the apparent Michaelis-Menten constant (K app M ) was calculated according to the Lineweaver-Burk equation [49] with the result as 2.77 mmol/L. Compared with other kinds of Hb modified electrodes, this K app M value is lower than that of some reported values such as CTS/ZnWO 4 -Hb/CILE (5.76 mmol/L) [50] and CTS/GR-LDHHb/CILE (8.48 mmol/L) [51], so the Hb molecules in CTS/3D-GR/CILE retained excellent bioactivity with a high biological affinity to TCA.…”

Section: Electrocatalytic Behavior Of Immobilized Hbmentioning

confidence: 85%

Direct electrochemistry and electrocatalysis of hemoglobin on three-dimensional graphene modified carbon ionic liquid electrode

Sun

Hou

Gong

et al. 2015

Sensors and Actuators B: Chemical

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“…21.171 (n = 26, c = 0.982). The detection limit was calculated as 0.174 mmol/L (3r), which was lower than that of the previous reported results of 0.200 mmol/L on Nafion/Mb/Co/CILE [7] and 0.613 mmol/L on CTS/ZnWO4-Hb/ CILE [39]. As the TCA concentration exceeded 119.4 mmol/L, the catalytic reduction peak currents began to level off and reached a plateau, implying a Michaelis-Menten kinetic process.…”

Section: Electrocatalytic Ability Of Nafion/mb-sa-fe 3 O 4 -Gr/cile Tmentioning

confidence: 96%

A novel biosensor based on electro-co-deposition of sodium alginate-Fe3O4-graphene composite on the carbon ionic liquid electrode for the direct electrochemistry and electrocatalysis of myoglobin

Chen

Yan

et al. 2016

Polym. Bull.

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A novel biosensor based on electro-co-deposition of myoglobin (Mb), sodium alginate (SA), Fe 3 O 4 -graphene (Fe 3 O 4 -GR) composite on the carbon ionic liquid electrode (CILE) was fabricated using Nafion as the film forming material to improve the stability of protein immobilized on the electrode surface, and the modified electrode was abbreviated as Nafion/Mb-SA-Fe 3 O 4 -GR/CILE. FT-IR and UV-vis absorption spectra suggested that Mb could retain its native structure after being immobilized in the SA-Fe 3 O 4 -GR composite film. The electrochemical behavior of the modified electrode was studied by cyclic voltammetry, and a pair of symmetric redox peaks appeared in the cyclic voltammograms, indicating that direct electron transfer of Mb was realized on the modified electrode, which was ascribed to the good electrocatalytic capability of Fe 3 O 4 -GR composite, the good biocompatibility of SA and the synergistic effects of SA and Fe 3 O 4 -GR composite. The electrochemical parameters of the electron transfer number (n), the charge transfer coefficient (a) and the electron transfer rate constant (k s ) were calculated as 0.982, 0.357 and 0.234 s -1 , respectively. The modified electrode exhibited good electrocatalytic ability to the reduction of trichloroacetic acid (TCA) with wide linear range from 1.4 to 119.4 mmol/L, low detection limit as 0.174 mmol/L (3r), good stability and reproducibility. X. Chen and H. Yan are co-first authors.

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Direct electrochemistry of hemoglobin on an ionic liquid carbon electrode modified with zinc tungstate nanorods

Cited by 13 publications

References 36 publications

Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic Activity

Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic Activity

Direct electrochemistry and electrocatalysis of hemoglobin on three-dimensional graphene modified carbon ionic liquid electrode

A novel biosensor based on electro-co-deposition of sodium alginate-Fe3O4-graphene composite on the carbon ionic liquid electrode for the direct electrochemistry and electrocatalysis of myoglobin

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