Direct determination of furaltadone metabolite, 3-amino-5-morpholinomethyl-2-oxazolidinone, in meats by a simple immunoassay

Abstract

Furaltadone is a banned drug for use in food producing animals and the marker residue of furaltadone in edible tissues is its metabolite, 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ). In this study, a novel polyclonal antibody of furaltadone was produced by using of the conjugate of furaltadone-bovine serum albumin as immunogen. The obtained antibody showed good specificity and sensitivity toward AMOZ with an IC 50 value of 2.3 ng/mL. Then, an indirect competitive immunoassay (ELISA) with heterologous coating antigen was developed to directly determine AMOZ in animal meat with a simple sample preparation. The limit of detection for AMOZ in meat was 0.4 ng/g. At three fortification levels, the intra-and inter-assay recoveries from blank samples were in a range of 85.0%Á103%. The ELISA method was further confirmed with a HPLC method in which AMOZ was determined after derivatization with 2-naphthaldehyde. The two methods showed good agreement from the analysis of incurred pork samples.

Rapid Detection of Tetracycline Residues in Duck Meat Using Surface Enhanced Raman Spectroscopy

“…It is highly imperative to detect all the duck products in advance in order to prevent the products containing the tetracycline residues from entering the markets. The conventional detection methods for the antibiotic residues in meat include enzyme-like immunosorbent assay [4][5][6], microbiological method [7,8], and physical and chemical detection method [9]. Although these methods have high detection precision, they cannot meet the rapid, mass, and field detection requirements of tetracycline residues in duck meat owing to the tedious pretreatment process or the timeconsuming detection process and so forth.…”

Development of Antibodies and Immunoassays for Monitoring of Nitrofuran Antibiotics in the Food Chain

“…Yan et al [48] took an alternative approach by manipulating the parent drug, FTD, to create a hapten. They reduced the nitro group to an amine using zinc powder dissolved in acetonitrile and methanol acidified to pH 5 while stirring at 40°C for 30 min.…”

Direct detection of 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) in food samples without derivatisation step by a sensitive and specific monoclonal antibody based ELISA

“…In the last decade, there have been a few ELISAs for the determination of nitrofurans and their derivatized metabolites (Chang, Peng, Wu, Wang, & Yuan, 2008;Cheng et al, 2009;Cooper, Caddell, Elliott, & Kennedy, 2004;Cooper, Samsonova, Plumpton, Elliott, & Kennedy, 2007;Gao, Chen, Cheng, Lei, & Zeng, 2007;Kotkova et al, 2005;Li, Liu, & Wang, 2009;Li, Liu, Zhang, Li, & Wang, 2010;Liu et al, 2007), and one ELISA using monoclonal antibody for the detection of NPAMOZ in spiked shrimp samples was reported (Umaporn et al, 2009). Recently, an indirect ELISA for the detection of AMOZ in meats using polyclonal antibody against parent furaltadone was also reported (Yan, Hu, Zheng, Liu, & Wang, 2011). In addition, other methods such as label-free electrochemical impedimetric immunosensor (Jin et al, 2011) and multiplexing biochip screening assay were reported for the detection of AMOZ or other nitrofuran metabolites (O'Mahony et al, 2011).…”