Direct detection of 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) in food samples without derivatisation step by a sensitive and specific monoclonal antibody based ELISA

Song, Juan; Yang, Hong; Wang, Yuzhen; Si, Wenhui; Deng, Anping

doi:10.1016/j.foodchem.2012.05.107

Cited by 27 publications

(26 citation statements)

References 31 publications

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“…Using the monoclonal antibody (mAb) against AMOZ produced in our lab [47], the aim of this study was to develop a novel ultrasensitive competitive ICA based on SERS for rapid detection of AMOZ without derivatization step in tissue and urine samples. The principle and procedures of this approach were similar to that of ICA which was based on normal use of GNPs ( …”

Section: Introductionmentioning

confidence: 99%

An ultrasensitive competitive immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for direct detection of 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) in tissue and urine samples

Li¹,

Yang²,

Li³

et al. 2015

Sensors and Actuators B: Chemical

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Section: Introductionmentioning

confidence: 99%

An ultrasensitive competitive immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for direct detection of 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) in tissue and urine samples

Li¹,

Yang²,

Li³

et al. 2015

Sensors and Actuators B: Chemical

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“…The formed metabolite derivatives were then typically chemically coupled to carrier proteins, via the carboxylic group, for the production of immunogens and coating antigens. In other studies, the metabolite AMOZ was also synthesized with 2-(4-formylphenoxy) acetic acid, 2-(3-formylphenoxy) acetic acid or 2-oxoacetic acid (Song et al, 2012;Xu et al, 2013). In the present study, AMOZ was synthesized with 4-CBA and the CPAMOZ-BSA conjugate was prepared using the active ester method.…”

Section: Discussionmentioning

confidence: 87%

A novel chemiluminescent ELISA for detecting furaltadone metabolite, 3-amino-5-morpholinomethyl-2-oxazolidone (AMOZ) in fish, egg, honey and shrimp samples

Liu

Jiang

Chen

et al. 2013

Journal of Immunological Methods

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“…Other ELISA methods were developed and applied to the determination of NPAMOZ in chicken, pork, fish and shrimp samples, reporting LOD values ranging from < 0.1 to 0.34 µg/kg (Luo et al, 2012;Xu et al, 2013;Shu et al, 2014). A number of ELISA methods have also been reported for direct determination of AMOZ, without derivatisation, with reported LOD values of 0.4 µg/kg (Song et al, 2012;Yan et al, 2012). ELISA methods have been described for the determination of 1([(2-nitrophenyl) methylene]-amine)-hydantoin (NPAHD) in pork, fish, shrimps and chicken with LOD values ranging between 0.09 and 0.15 µg/kg (Wenxiao et al, 2012) and in shrimps with an LOD value of 0.11 µg/kg (Chadseesuwan et al, 2013).…”

Section: Screening Methodsmentioning

confidence: 99%

Scientific Opinion on nitrofurans and their metabolites in food

Panel¹

2015

EFS2

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Nitrofurans are antimicrobial agents not authorised for use in food-producing animals in the European Union. Nitrofurans are rapidly metabolised, occurring in animal tissues as protein-bound metabolites. The European Commission requested EFSA to provide a scientific opinion on the risks to human health related to the presence of nitrofurans in food and whether a reference point for action (RPA) of 1.0 µg/kg for the marker metabolites is adequate to protect public health. Data on occurrence of nitrofuran marker metabolites in food were extracted from the national residue monitoring plan results and from the Rapid Alert System for Food and Feed (RASFF). The CONTAM Panel concluded that these data were too limited to carry out a reliable human dietary exposure assessment. Instead, human dietary exposure was calculated for a scenario in which a single nitrofuran marker metabolite is present at 1.0 µg/kg in foods of animal origin, excluding milk and dairy products. The mean chronic dietary exposure for this worst-case scenario would range from 3.3 to 8.0 and 1.9 to 4.3 ng/kg b.w. per day for toddlers and adults, respectively. Nitrofurans and their marker metabolites, generally, are genotoxic and carcinogenic and, also, have non-neoplastic effects in animals. Margins of exposure (MOEs) were calculated at 2.0 × 10 5 or greater for carcinogenicity and at 2.5 × 10 3 or greater for non-neoplastic effects. The CONTAM Panel concluded that it is unlikely that exposure to food contaminated with nitrofuran marker metabolites at or below 1.0 µg/kg is a health concern. A scenario in which foods are considered to be contaminated with semicarbazide, from use of carrageenan as a food additive, at 1 µg/kg was used to assess whether it is appropriate to apply the RPA to foods of non-animal origin; MOEs of greater than 10 4 calculated for nonneoplastic effects do not indicate a health concern.© European Food Safety Authority, 2015 However, the opinion also identified certain categories of non-allowed pharmacologically active substances that are considered to be outside the scope of the procedure, including substances that are high potency carcinogens, such as nitrofurans. As nitrofurans are excluded from that opinion, and taking into account that the presence of SEM in food may be from sources other than use of nitrofurazone, the European Commission (EC) requested the European Food Safety Authority (EFSA) for a scientific opinion on the risks to human health related to the presence of nitrofurans and their metabolites in food. The opinion should include (a) an evaluation of the toxicity of nitrofurans and their metabolites for humans, considering all relevant toxicological endpoints and identification of the toxicological relevance of nitrofurans and their metabolites present in food, and (b) an exposure assessment of the EU population to nitrofurans and their metabolites from food, including the consumption patterns of specific (vulnerable) groups of the population. In addition, the opinion should assess the appropriateness of...

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Direct detection of 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) in food samples without derivatisation step by a sensitive and specific monoclonal antibody based ELISA

Cited by 27 publications

References 31 publications

An ultrasensitive competitive immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for direct detection of 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) in tissue and urine samples

An ultrasensitive competitive immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for direct detection of 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) in tissue and urine samples

A novel chemiluminescent ELISA for detecting furaltadone metabolite, 3-amino-5-morpholinomethyl-2-oxazolidone (AMOZ) in fish, egg, honey and shrimp samples

Scientific Opinion on nitrofurans and their metabolites in food

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