A novel chemiluminescent ELISA for detecting furaltadone metabolite, 3-amino-5-morpholinomethyl-2-oxazolidone (AMOZ) in fish, egg, honey and shrimp samples

Liu, Yingchun; Jiang, Wei; Chen, Yongjun; Yan, Xilong; Shi, Jin-Lei; Qiao, Yansong; Zhang, Huajing; Li, Tao; Wang, Quan

doi:10.1016/j.jim.2013.06.006

Cited by 24 publications

(18 citation statements)

References 30 publications

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“…A number of ELISA methods have been reported for the determination of NPAMOZ in shrimps and fish samples with reported LOD values of 0.1-0.3 µg/kg and LOQ or CCβ values of 0.3-0.36 µg/kg (Pimpitak et al, 2009;Shen et al, 2012;Sheu et al, 2012;Yang et al, 2012;Liu et al, 2013). Other ELISA methods were developed and applied to the determination of NPAMOZ in chicken, pork, fish and shrimp samples, reporting LOD values ranging from < 0.1 to 0.34 µg/kg (Luo et al, 2012;Xu et al, 2013;Shu et al, 2014).…”

Section: Screening Methodsmentioning

confidence: 99%

Scientific Opinion on nitrofurans and their metabolites in food

Panel¹

2015

EFS2

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Nitrofurans are antimicrobial agents not authorised for use in food-producing animals in the European Union. Nitrofurans are rapidly metabolised, occurring in animal tissues as protein-bound metabolites. The European Commission requested EFSA to provide a scientific opinion on the risks to human health related to the presence of nitrofurans in food and whether a reference point for action (RPA) of 1.0 µg/kg for the marker metabolites is adequate to protect public health. Data on occurrence of nitrofuran marker metabolites in food were extracted from the national residue monitoring plan results and from the Rapid Alert System for Food and Feed (RASFF). The CONTAM Panel concluded that these data were too limited to carry out a reliable human dietary exposure assessment. Instead, human dietary exposure was calculated for a scenario in which a single nitrofuran marker metabolite is present at 1.0 µg/kg in foods of animal origin, excluding milk and dairy products. The mean chronic dietary exposure for this worst-case scenario would range from 3.3 to 8.0 and 1.9 to 4.3 ng/kg b.w. per day for toddlers and adults, respectively. Nitrofurans and their marker metabolites, generally, are genotoxic and carcinogenic and, also, have non-neoplastic effects in animals. Margins of exposure (MOEs) were calculated at 2.0 × 10 5 or greater for carcinogenicity and at 2.5 × 10 3 or greater for non-neoplastic effects. The CONTAM Panel concluded that it is unlikely that exposure to food contaminated with nitrofuran marker metabolites at or below 1.0 µg/kg is a health concern. A scenario in which foods are considered to be contaminated with semicarbazide, from use of carrageenan as a food additive, at 1 µg/kg was used to assess whether it is appropriate to apply the RPA to foods of non-animal origin; MOEs of greater than 10 4 calculated for nonneoplastic effects do not indicate a health concern.© European Food Safety Authority, 2015 However, the opinion also identified certain categories of non-allowed pharmacologically active substances that are considered to be outside the scope of the procedure, including substances that are high potency carcinogens, such as nitrofurans. As nitrofurans are excluded from that opinion, and taking into account that the presence of SEM in food may be from sources other than use of nitrofurazone, the European Commission (EC) requested the European Food Safety Authority (EFSA) for a scientific opinion on the risks to human health related to the presence of nitrofurans and their metabolites in food. The opinion should include (a) an evaluation of the toxicity of nitrofurans and their metabolites for humans, considering all relevant toxicological endpoints and identification of the toxicological relevance of nitrofurans and their metabolites present in food, and (b) an exposure assessment of the EU population to nitrofurans and their metabolites from food, including the consumption patterns of specific (vulnerable) groups of the population. In addition, the opinion should assess the appropriateness of...

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Section: Screening Methodsmentioning

confidence: 99%

Scientific Opinion on nitrofurans and their metabolites in food

Panel¹

2015

EFS2

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“…However, those methods require a longer time to perform (Liu et al, 2013). In this study, enzyme-linked immunosorbent assay (ELISA) method was applied.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, enzyme-linked immunosorbent assay (ELISA) method was applied. ELISA method has been successfully detected various kinds of medicine residues, such as furazolidone in fish (Jester et al, 2014), furaltadone metabolites in shrimp (Liu et al, 2013), and 19-Nortestosterone in animal tissues (Jiang et al, 2011). This study aimed to determine the MT immersion dose that allows the highest percentage of males, growth, and survival.…”

Section: Introductionmentioning

confidence: 99%

The sex ratio and testosterone levels in tilapia immersed in different doses of 17α-methyltestosterone

Zairin¹,

Naufal²,

Setiawati³

et al. 2017

Jurnal Akuakultur Indonesia

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Nile tilapia farming using monosex male population has been reported to improve the productivity by 10% compared to that of mix-sex culture. This study aimed to determine an immersion dose of 17α-metiltestosterone (MT) that resulted in higher male percentage, growth, survival, and to measure testosterone level in the fish body. The experiment compared three MT immersion doses, namely: 0; 1.8; and 5.4 mg/L, each with three replications. Immersion was conducted to 14-days-old larvae for four hours, with a density of 100 fish/L of water. Testosterone levels in fish was measured using ELISA method, and sex identification was performed by histological method. The result showed that the percentage of male fish was the same in treatments 1.8 mg/L and 5.4 mg/L, which were 53-65% higher than that of the control without MT treatment. Fish growth and survival until day 56 post immersion were not significantly different amongst treatments. The results of ELISA analysis showed that the levels of testosterone in larvae just after immersion was similar in 1.8 mg/L and 5.4 mg/L treatments, which then drastically decreased on day 14 after immersion. On day 28 after immersion the levels of testosterone of the MT treatments were similar to that of the control, i.e. about 2 ng/g. On day 71 the testosterone levels in sex reversed and normal males fish remained the same at about 0.3 ng/g (P>0.05). By PCR method with specific primer, sex reversed and normal males could be distinguished. In conclusion, sex reversal by immersion at a dose of 1.8 mg/L can be considered as a standard protocol for Nile tilapia sex reversal. Testosterone level in the body of MT-treated fish could be reduced to the same level as the control in less than one month post immersion.Keywords: sex reversal, 17α-methyltestosterone, testosterone levels, immersion, tilapia ABSTRAK Budidaya ikan nila menggunakan sistem monoseks jantan telah dilaporkan dapat meningkatkan produktivitas hingga 10%. Penelitian ini dilakukan menentukan dosis perendaman hormon 17α-metiltestosteron (MT) yang menghasilkan rasio ikan jantan tertinggi, pertumbuhan, sintasan, dan mengukur kadar testosteron dalam tubuh ikan nila. Dosis hormon MT yang diuji adalah 0; 1,8; dan 5,4 mg/L. Setiap perlakuan dosis diberi tiga ulangan. Perendaman dilakukan selama empat jam, dengan jumlah larva (umur 14 hari pascatetas) 100 ekor dalam 1 L air. Hasil analisis histologi menunjukkan bahwa persentase ikan nila jantan adalah sama pada perlakuan 1,8 mg/L dan 5,4 mg/L, dan 53-65% lebih tinggi daripada kontrol tanpa direndam MT (54% jantan). Dengan analisis ELISA, kadar testosteron dalam tubuh larva sesaat setelah perendaman MT dosis 1,8 mg/L dan 5,4 mg/L adalah sama, tetapi turun drastis pada hari ke-14 pascarendam, kemudian kadarnya menjadi relatif sama pada hari ke-28, yaitu 2 ng/g). Pertumbuhan bobot dan sintasan ikan sampai hari ke-56 adalah sama. Dengan menggunakan metode PCR, jantan fungsional dan jantan normal dapat dibedakan, dan pada hari ke-71 kadar testosteron ikan jantan fungsional dan jantan...

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“…Compared to the traditional colorimetric immunoassays, CL‐ELISA offers the possibility of improved sensitivity with the enhanced chemiluminescent reaction (ECR) . The CL‐ELISA methods are broadly applied in the area of fast food safety inspection . However, to the best of our knowledge, there are no related reports about simultaneous determination of T‐2 and HT‐2 applying CL‐ELISA method.…”

Section: Introductionmentioning

confidence: 99%

“…25,26 The CL-ELISA methods are broadly applied in the area of fast food safety inspection. 27,28 However, to the best of our knowledge, there are no related reports about simultaneous determination of T-2 and HT-2 applying CL-ELISA method.…”

Section: Introductionmentioning

confidence: 99%