No abstract

Furaltadone is a banned drug for use in food producing animals and the marker residue of furaltadone in edible tissues is its metabolite, 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ). In this study, a novel polyclonal antibody of furaltadone was produced by using of the conjugate of furaltadone-bovine serum albumin as immunogen. The obtained antibody showed good specificity and sensitivity toward AMOZ with an IC 50 value of 2.3 ng/mL. Then, an indirect competitive immunoassay (ELISA) with heterologous coating antigen was developed to directly determine AMOZ in animal meat with a simple sample preparation. The limit of detection for AMOZ in meat was 0.4 ng/g. At three fortification levels, the intra-and inter-assay recoveries from blank samples were in a range of 85.0%Á103%. The ELISA method was further confirmed with a HPLC method in which AMOZ was determined after derivatization with 2-naphthaldehyde. The two methods showed good agreement from the analysis of incurred pork samples.