Differential expression of the neural cell adhesion molecule NCAM 140 in human pituitary tumors

Aletsee-Ufrecht, M. C.; Langley, Keith; Gratzl, O.; Gratzl, Manfred

doi:10.1016/0014-5793(90)80445-o

Cited by 23 publications

(2 citation statements)

References 23 publications

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“…It is known that progression to neoplasia involves the ability to adhere and interact with the surrounding cells and extracellular matrix, and loss or adhesion difficulties may be a determinant of epithelial neoplasia ( 36 ). Studies have demonstrated immunohistochemical expression of NCAM in pituitary adenomas, including GH-secreting tumors ( 5 , 37 – 39 ).…”

Section: Discussionmentioning

confidence: 99%

Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly

Mendes

Haag

Trott

et al. 2018

Braz J Med Biol Res

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Pituitary adenomas account for 10–15% of primary intracranial tumors. Growth hormone (GH)-secreting adenomas account for 13% of all pituitary adenomas and cause acromegaly. These tumors can be aggressive, invade surrounding structures and are highly recurrent. The objective of this study was to evaluate E-cadherin, Slug and neural cell adhesion molecule (NCAM) expression in GH-secreting pituitary adenomas and its relationship to tumor invasiveness. A cross–sectional study of patients who underwent hypophysectomy due to GH-secreting pituitary adenoma from April 2007 to December 2014 was carried out. The medical records were reviewed to collect clinical data. Immediately after surgery, tumor samples were frozen in liquid nitrogen and stored in a biofreezer at –80°C for assessment of E-cadherin 1 (CDH1), SLUG (SNAI2), and NCAM (NCAM1) by real-time PCR. The samples were fixed in formalin and embedded in paraffin for immunohistochemical analysis of E-cadherin and NCAM. Thirty-five patients with acromegaly were included in the study. Of these, 65.7% had invasive tumors. Immunohistochemically, E-cadherin was expressed in 96.7% of patients, and NCAM in 80% of patients. There was no statistically significant relationship between tumor grade or invasiveness and immunohistochemical expression of these markers. Regarding gene expression, 50% of cases expressed CDH1, none expressed SNAI2, and 53.3% expressed NCAM1. There was no statistically significant relationship between tumor grade or invasiveness and gene expression of CDH1, SNAI2, and NCAM1. The absence of Slug overexpression and of E-cadherin and NCAM suppression suggests that expression of these markers is not associated with tumor invasiveness in GH-secreting pituitary adenomas.

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Section: Discussionmentioning

confidence: 99%

Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly

Mendes

Haag

Trott

et al. 2018

Braz J Med Biol Res

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“…NCAMs were first found in the nervous system, but subsequently were also found in polypeptide hormoneand steroid-producing endocrine cells [10][11][12][13][14][15]. NCAMs allow the formation of cell clusters by auto-adhesion of neighboring NCAM-bearing cells.…”

Section: Introductionmentioning

confidence: 99%

Expression and alternative splicing of the neural cell adhesion molecule NCAM in human granulosa cells during luteinization

1994

FEBS Letters

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Freshly aspirated human granulosa cells from pre-ovulatory follicles and granulosa cells luteinized in culture possess the neural cell adhesion molecule (NCAM) of approximate molecular mass of 140,000 and NCAM mRNA as confirmed by Sl-nuclease protection assays and RT-PCR. Moreover, in the process of luteinization the NCAM isoform pattern is modified. Isoforms containing an insert of 10 amino acids (termed VASE) in the extracellular domain of NCAM were supplemented by alternatively spliced isoforms without this insert. NCAM immunoreactivity, at light and electron microscope levels, was associated with the cell membrane of most granulosa cells which formed clusters. During time in culture an increasing subpopulation of granulosa cells, devoid of NCAM immunoreactivity, spread out and formed monolayers. This differential expression and the alternative splicing of NCAM during luteinization of granulosa cells raise the possibility that NCAM could be involved in folliculogenesis and the formation of the corpus luteum in the human.

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Sialobiology and the Polysialic Acid Glycotope Occurrence, Structure, Function, Synthesis, and Glycopathology

Troy

1995

Biology of the Sialic Acids

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Differential expression of the neural cell adhesion molecule NCAM 140 in human pituitary tumors

Cited by 23 publications

References 23 publications

Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly

Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly

Expression and alternative splicing of the neural cell adhesion molecule NCAM in human granulosa cells during luteinization

Sialobiology and the Polysialic Acid Glycotope Occurrence, Structure, Function, Synthesis, and Glycopathology

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