Expression and alternative splicing of the neural cell adhesion molecule NCAM in human granulosa cells during luteinization

doi:10.1016/0014-5793(94)00473-0

Cited by 26 publications

(1 citation statement)

References 35 publications

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“…CD56 is a membrane-bound cell surface sialoglycoprotein and a member of the immunoglobulin supergene family, which induces cell-to-cell interactions during embryonic development, cell migration, and organogenesis (37). …”

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confidence: 99%

Granulosa Cell Tumors: Novel Predictors of Recurrence in Early-stage Patients

Sakr

Abdulfatah

Thomas

et al. 2017

International Journal of Gynecological Pathology

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Summary: Granulosa cell tumors (GCTs) comprise 2% to 5% of ovarian neoplasms, with unpredictable patterns of recurrence. The HER family, GATA4, and SMAD3 genes are reportedly involved in GCT proliferation and apoptosis and may serve as new predictors of recurrence. The aim of the study was to evaluate novel predictors of recurrence in GCT from a large single institution cohort. Patients diagnosed with GCTs (n = 125) between 1975 and 2014 were identified. Clinicopathologic parameters were obtained and immunohistochemical evaluation was performed of calretinin, inhibin, HER2, CD56, SMAD3, and GATA4. Statistical analyses were conducted using Fisher exact test and Kaplan-Meier survival curves and Cox regression analysis. The median follow-up period was 120 months (range, 1–465 mo). Recurrence was noted in 12/125 (9.6%) patients. Kaplan-Meier analysis showed a shorter mean disease-free interval in whites versus blacks (P = 0.001), stage III-IV versus stage I-II (P = 0.0001), patients treated with surgery+chemotherapy versus surgery (P = 0.0001), mitotic rate ≥4 (P = 0.005), severe nuclear pleomorphism (P = 0.013), high HER2 expression (P = 0.001), high CD56 expression (P = 0.001), and high SMAD3 expression (P = 0.001). On Cox regression analysis, SMAD3 and type of treatment received were the only 2 independent prognostic factors for disease-free interval (P = 0.03 and P = 0.007, respectively). On subanalysis for early-stage (stage I) GCTs, the need for adjuvant chemotherapy and high expression of SMAD3 continued to be independent predictors of recurrence (HR = 10.2, P = 0.01 and HR = 8.9, P = 0.001, respectively).

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confidence: 99%

Granulosa Cell Tumors: Novel Predictors of Recurrence in Early-stage Patients

Sakr

Abdulfatah

Thomas

et al. 2017

International Journal of Gynecological Pathology

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The Neural Cell Adhesion Molecule (NCAM) Provides Clues to the Development of Testicular Leydig Cells

MAYERHOFER,

LAHR,

SEIDL

et al. 1996

Journal of Andrology

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As previously shown, Leydig cells in culture dramatically up‐regulate the expression of the neural cell adhesion molecule (NCAM) gene and express alternatively spliced forms. Because the family of NCAM adhesion molecules is known to be involved in cell migration and differentiation, we examined the potential involvement of NCAMs in Leydig cell differentiation in the developing testis. We detected NCAM‐immunoreactive cells in the rat and mouse at embryonic day (ED) 13–14 in epithelia of mesonephric tubules and cell clusters between the mesonephros and testis. At about ED 17–18, strongly NCAM‐immunoreactive cells were demonstrated extending from the mesonephros/mesonephric tubules into the region occupied by the forming rete testis and spreading into the testis itself. Within the testis, interstitial fetal Leydig cells, identified with an antiserum directed against P450‐side‐chain cleavage enzyme, were also NCAM immunoreactive, although to a lesser degree, and in part expressed NCAM‐associated polysialic acid. In situ hybridization histochemistry demonstrated the presence of NCAM mRNA, mainly in the cell population corresponding to the strongly immunoreactive cells. NCAM forms with molecular weights of 140 and 180 kDa, the latter polysialylated, predominate in immunoblots of fetal testes. Because testicular development occurs along the mesonephros, from which precursor cells are thought to migrate into the developing gonad and then differentiate into the various testicular cell types, our results may suggest that the expression of NCAMs and NCAM modification are associated with cells that appear to migrate into the developing testis. Although it remains to be proven whether these cells could be precursor cells of Leydig cells, this assumption is supported by the fact that within the developing testis, NCAMs and NCAM modifications are expressed during differentiation of testicular P450‐side‐chain cleavage enzyme‐positive Leydig cells. Thus, the adhesion molecule NCAM and its variants and modifications are expressed in the developing testis and may be of functional, “morphogenic” importance. Because Leydig cells in vitro and during fetal development express NCAMs, these molecules may prove to be a suitable specific marker for the study of Leydig cell development and differentiation.

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Immunocytochemical analysis of the expression of gap junction protein connexin 43 in the rat ovary

Mayerhofer

Garfield

1995

Molecular Reproduction Devel

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The present immunocytochemical study examines in the rat ovary the pattern of expression of connexin 43 (Cx43), a subunit of gap junctions. Using a well-characterized specific antiserum against rat Cx43, immunoreactivity was not detected in the fetal ovary, i.e., prior to follicular formation. However, in the ovary of 20-day-old, 35-day-old, and adult rats, strong Cx43-immunoreactivity was associated with the cell borders of the follicular epithelium/granulosa cells of all developmental stages (primordial follicles, preantral and antral secondary follicles). In general, immunoreactivity of the granulosa cells of large antral follicles appeared more intense than the one of smaller follicles. Staining was also seen in oocytes (cytoplasmic staining). Theca cells of large antral follicles, but not of small follicles were immunoreactive. Immunoreactive interstitial cells were not seen in ovaries of 20- and 35-day-old animals, but staining in these cells was present in adult rats. In large follicles with signs of atresia, granulosa cells lacked Cx43-immunoreactivity, whereas Cx43-immunoreactivity in their theca interna strikingly increased. Corpora lutea in the cyclic adult rats were heterogeneously stained, with either no detectable immunoreactivity, staining of cell borders of most luteal cells, or with conspicuous staining of only a few cells. In the pregnant animals on gestation days (GD) 12, 14, and 17, all luteal cells stained strongly for Cx43 at the cell surface.(ABSTRACT TRUNCATED AT 250 WORDS)

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Expression and alternative splicing of the neural cell adhesion molecule NCAM in human granulosa cells during luteinization

Cited by 26 publications

References 35 publications

Granulosa Cell Tumors: Novel Predictors of Recurrence in Early-stage Patients

Granulosa Cell Tumors: Novel Predictors of Recurrence in Early-stage Patients

The Neural Cell Adhesion Molecule (NCAM) Provides Clues to the Development of Testicular Leydig Cells

Immunocytochemical analysis of the expression of gap junction protein connexin 43 in the rat ovary

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