Differences in in vitro Pollen Germination and Pollen Tube Growth of Cotton Cultivars in Response to High Temperature

Kakani, Vijaya Gopal; Reddy, K. R.; Koti, Sailaja; Wallace, T.; Prasad, P. V. Vara; Reddy, V. R.; Zhao, Duli

doi:10.1093/aob/mci149

Cited by 249 publications

(270 citation statements)

References 41 publications

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“…The differences found between in vitro pollen viability studies in relation to the yellow passion fruit are probably due to the genetic origin of the material itself, environmental conditions and the culture mediums used for germination. Kakani et al (2005) confirms that the differences observed during in vitro pollen germination and the growth of the pollen tube in relation to twelve cotton cultivars (Gossypium hirsutum) were, in fact, related to variations in the cultivars themselves. Similarly, Franzon et al (2005) working with feijoa (Acca sellowiana (Berg) Burret) reported differences between species and between cultivars within the same species, in relation to the necessary culture medium conditions for in vitro pollen germination.…”

Section: Discussionsupporting

confidence: 74%

Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)

et al. 2013

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The characterization and viability of pollen grains are useful tools to guide crosses in breeding programs. The objective of this study was to describe the morphological patterns and viability of pollen grains from five accessions of Passiflora edulis f. flavicarpa O. Deg. and five accessions of Passiflora setacea DC. Pollen morphology descriptions were made using light microscopy and scanning electron microscopy, whereas the viability analysis was performed by in vitro germination and histochemical analysis (Lugol's solution and 2,3,5-triphenyltetrazolium chloride). Pollen grains assessed for germination were inoculated in culture medium containing Ca(NO 3 ).4H 2 O (0.03%), Mg(SO 4 ).7H 2 O (0.02%), KNO 3 (0.01%), H 3 BO 3 (0.01%), sucrose (15%) and agar (0.8%). Although P. edulis and P. setacea showed the same shape and type of pollen aperture, the two differed in terms of their morphology and exine ornamentation pattern. In vitro analysis showed that one of the P. edulis f. flavicarpa accessions (designated BGP 330) presented the highest germination rate (53.98%) and longest pollen tube (2.18 mm). The histochemical analysis overestimated pollen viability when compared with the in vitro results. The results of this study contribute to the breeding of Passiflora species by increasing the understanding of their morphology and pollen grain viability.

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Section: Discussionsupporting

confidence: 74%

Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)

et al. 2013

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“…For example, while for many species native to or cultivated in temperate regions optimum temperatures in the range 15ºC-25ºC are usually recorded [examples include Arabidopsis thaliana (22ºC, Boavida and McCormick, 2007), sweet cherry (25ºC, Lewis, 1942), or almond (Prunus dulcis Mill.) and peach (16ºC and 23ºC respectively, Weinbaum et al, 1984)], optimum temperatures above 25ºC have been reported for several subtropical and tropical species such as Datura stramonium (33ºC, Buchholz and Blakeslee, 1927), Oenothera (33ºC, Lewis, 1942), groundnut (30ºC for pollen germination and 34ºC for pollen tube growth, Kakani et al, 2002) or cotton (Gossypium hirsutum L.) (from 28ºC to 32ºC, Kakani et al, 2005). These adaptations can be best seen in natural populations that flower during extremely high or low temperatures.…”

Section: Genetic Variation In the Reproductive Processes Under Tempermentioning

confidence: 99%

“…Genotype-specific responses have been reported for stress applied during microsporogenesis affecting carbohydrate metabolism, degeneration of the tapetal tissues, pollen wall architecture, pollen morphology, pollen viability, anther dehiscence and pollen production (Aloni et al, 2001;Koti et al, 2005;Oliver et al, 2005;Porch and Jahn, 2001;Prasad et al, 2006;Pressman et al, 2002;Srinivasan et al, 1999;Suzuki et al, 2001). At the post-anthesis level, pollen germination (Hedhly et al, 2005a;Kakani et al, 2002Kakani et al, , 2005, pollen tube growth rate (Clarke and Siddique, 2004;Hedhly et al, 2004;Srinivasan et al, 1999), and pollen dynamics (Hedhly et al, 2005a) also show genotype-specific responses.…”

Section: Genetic Variation In the Reproductive Processes Under Tempermentioning

confidence: 99%

Sensitivity of flowering plant gametophytes to temperature fluctuations

Hedhly

2011

Environmental and Experimental Botany

252

179

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Research on plant responses to temperature stress is receiving increased interest due to the growing awareness about global warming. High and low temperature stresses help establish the narrow geographic distribution of some cultivated plants, the limited geographic extension of some other economically nutritionally important species, and also induce irregular bearing for some species. However, the understanding of plant responses to temperature stress lags behind other biotic and abiotic stresses probably due to the complex response at the molecular, cellular, and organismal level. Temperature stress affects, indeed, many developmental processes during the plant's life cycle. However, the reproductive stage, the outcome of which represents the economic value for many cultivated plants, is especially vulnerable. Here the effect of low and high temperature stresses during the flowering phase is reviewed in flowering plants in an attempt to unravel sensitive stages that are behind irregular cropping. The review presents detailed findings from 33 previously published reports spanning 19 different flowering plant species. Both the male and female organs of the flower are especially sensitive to temperature fluctuations both during their development before pollination and during the post-pollination stage. The effect of temperature stress is, however, obscured by the complex male-female interaction superimposed on the individual behavior of each organ. Interestingly, a review of the literature on this topic shows that genetic variation does exist in reproductive behavior under temperature fluctuations. This genetic diversity must be preserved and characterized in further detail to understand how plants naturally cope with changing environmental conditions, which will, undoubtedly, help us to design better strategies to face current and future challenging temperature fluctuations.

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“…Temperature is considered one of the environmental factors that most influences the reproductive process of plants (Kakani et al, 2005). To test the response of in vitro pollen grain germination and pollen tube growth, studies examining variations in temperature and the use of linear and non-linear regression models have been widely used.…”

Section: Resultsmentioning

confidence: 99%

“…This subject has been studied in several different crops such as pistachios (Acar and Kakami, 2010), peaches (Hedhly et al, 2005), peanuts (Kakani et al, 2002), cotton (Kakani et al, 2005), sorghum (Prasad et al, 2011), Capsicum (Reddy and Kakani, 2007) and papaya (Tamaki et al, 2011). Based on their findings, these authors have defined the cardinal temperatures: the minimum temperature (T min ) at which pollen grains germinate, the optimal temperature at which the pollen grain germination reaches the maximum (T opt ) and the maximum temperature (T max ) after which pollen grain germination does not occur.…”

Section: Introductionmentioning

confidence: 99%

Determination of cardinal temperatures for sugar apple ( Annona squamosa L.)

et al. 2016

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The sugar apple is highly dependent on the pollination and fruit tree pollen performance is crucial for successful fertilization and fruit set. The objective of this study was to determine the cardinal temperatures for in vitro pollen grains germination and evaluate the effect of temperature on pollen tube growth of sugar apple. The experimental design was in a completely randomized with eight treatments (10, 15, 20, 25, 30, 35, 40 and 45 ºC), four replicates and each plot was constituted by two slides per parcel. The cardinal temperatures were determined by evaluating in vitro germination of pollen grains and pollen tube growth in standard culture medium. It also assessed the pollen tube growth and the percentage of germination in vitro depending on the type of pollen grain arrangement (monad, dyad, triad and tetrad). In vitro germination of pollen grains and pollen tube growth varied significantly with temperature. The maximum germination recorded (48.13 %) and the maximum lengths of pollen tubes (536.45 μm) were obtained when pollen grains were cultivated at 25 ºC. The estimated cardinal temperatures were 9.7, 26.9 and 44.2 ºC. Among the pollen grain arrangements, tetrads pollen grains were observed in higher proportions, however, monads pollen grains presented higher germination percentage. Index terms:In vitro germination; pollen viability; monads; tetrads; pollen tube. RESUMOA pinheira é uma fruteira altamente dependente da polinização e o desempenho do pólen é determinante para o sucesso da fertilização e frutificação efetiva. O objetivo deste trabalho foi determinar as temperaturas cardinais para a germinação in vitro dos grãos de pólen e avaliar o efeito de diferentes temperaturas no crescimento dos tubos polínicos de pinheira. O delineamento experimental foi o inteiramente casualizado, com oito tratamentos (10, 15, 20, 25, 30, 35, 40 e 45 ºC), quatro repetições, sendo cada parcela experimental constituída por duas lâminas. As temperaturas cardinais foram determinadas por meio da avaliação da germinação in vitro dos grãos de pólen e do crescimento do tubo polínico em meio de cultura padrão. Avaliou-se também o crescimento do tubo polínico e o percentual de germinação in vitro em função do tipo de arranjo do grão de pólen (mônade, díade, tríade e tétrade germinados). A germinação in vitro dos grãos de pólen e o crescimento dos tubos polínicos da pinha variou significativamente com a temperatura. A máxima germinação in vitro registrada (48,13 %) e o máximo comprimento dos tubos polínicos (536,45 µm) foram obtidos quando os grãos de pólen foram cultivados a 25 °C. As temperaturas cardinais estimadas foram 9,7, 26,9 e 44,2 ºC. Dentre os arranjos de grãos de pólen, foi observada maior percentagem de tétrades, no entanto, os grãos de pólen do tipo mônades apresentaram maiores porcentagem de germinação.Termos para indexação: Germinação in vitro; viabilidade polínica; mônades; tétrades; tubo polínico.

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Differences in in vitro Pollen Germination and Pollen Tube Growth of Cotton Cultivars in Response to High Temperature

Abstract: Based on principal component analysis, it is concluded that higher pollen germination percentages and longer pollen tubes under optimum conditions and with optimum temperatures above 32 degrees C for pollen germination would indicate tolerance to high temperature.

Cited by 249 publications

References 41 publications

Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)

Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)

Sensitivity of flowering plant gametophytes to temperature fluctuations

Determination of cardinal temperatures for sugar apple ( Annona squamosa L.)

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