Diet high in α-linolenic acid up-regulate PPAR-α gene expression in the liver of goats

Abstract

Background: There is little information on the effects of diets containing high α-linolenic acid (C18:3n-3) on liver lipid composition and lipogenic gene expressions. In this study fourteen goats (Capra aegagrus hircus) were fed either a flaxseed oil (FSO) supplemented diet containing high α-linolenic acid or a control diet without added flaxseed oil (CON) for 100-d to evaluate the effects on liver lipid composition and the gene expression of peroxisome proliferator-activated receptors (PPAR-α) and stearoyl-CoA-desaturase (SCD) in the liver. Results: An increase in the levels of C18:3n-3 and C20:5n-3, C22:5n-3, C22:6n-3 was observed in the liver of FSO-treated goats. There was a significant (P b 0.05) up-regulation of PPAR-α gene expression and downregulation of SCD gene in the liver of goats fed the high α-linolenic acid diet. Conclusions: In conclusion, genes associated with the control of fatty acid (FA) conversion (SCD and PPAR) were affected by the α-linolenic acid supplementation in the goat diet. It is suggested that PPAR-α is the key messenger responsible for the translation of nutritional stimuli into changes in hepatic gene expression.

Effects of dietary n-6: n-3 polyunsaturated fatty acid ratios on meat quality, carcass characteristics, tissue fatty acid profiles, and expression of lipogenic genes in growing goats

“…For FA analysis, total fat was extracted from the experimental samples and tissue using chloroform: methanol following the method described by Ebrahimi et al [ 22 ]. The extracted fat was saponified with ethanolic KOH for 10 min at 90°C.…”

Influence of Maternal and Postweaning Linseed Dietary Supplementation on Growth Rate, Lipid Profile, and Meat Quality Traits of Light Sarda Lambs

“…Even if these results cannot be entirely transposed to the ovine species, it can be hypothesized that a similar epigenetic regulation of the FA synthesis exists. In a recent study with goats fed with a linseed oil-supplemented diet, Ebrahimi et al [ 15 , 44 ] found an effect on the expression of the PPAR- α gene associated with the regulation of FA metabolic pathway and a downregulation of the SCD gene expression.…”

“…Hoile et al [ 11 ] have demonstrated that maternal fat intake can alter the epigenetic regulation of Fads2 transcription in rats. In goats, high α -linolenic acid diets were able to upregulate the peroxisome proliferator-activated receptors (PPAR- α ) gene and downregulate the stearoyl-CoA-desaturase gene compared to control diets with low ALA content [ 15 ]. In a study conducted with Vendeen sheep, extruded linseed- (EL-) enriched concentrates used in both maternal and lamb weaning diets were able to increase the proportions of α -linolenic acid (ALA) and n-3 PUFA in tissues [ 16 ].…”

Pengaruh Intervensi Tahu Kedelai Hitam Kaya Serat Terhadap Glukosa Darah Dan Inflamasi Responden Diabetes Tipe 2

“…*uji nonparametrik Man-Whitney Penelitian preklinis Handayani et al (2009) mengemukakan bahwa pemberian susu kedelai dapat menurunkan kadar glukosa darah postprandial tikus jantan secara signifikan, dimana polisakarida dan asam lemak omega-3 pada kedelai berperan dalam mengatur homeostatis glukosa darah. Menurut Ebrahimi et al (2015), asam lemak omega-3 dapat meningkatkan regulasi PPAR-γ ang dapat meningkatkan ekspresi gen yang mengkode GLUT-4 (tempat masuknya glukosa ke dalam sel). Selain protein, serat dan asam lemak esensial, lesitin kedelai juga berfungsi menurunkan kadar glukosa darah.…”