Dielectric inspection of erythrocyte morphology

Hayashi, Yoshihito; Oshige, Ikuya; Katsumoto, Yoichi; Omori, Shinji; Yasuda, Akio; Asami, Kōji

doi:10.1088/0031-9155/53/10/007

Cited by 48 publications

(61 citation statements)

References 25 publications

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“…In the present paper, referring to our recently reported experimental data, we first demonstrate the capability of the DS to differentiate suspensions of different cell shapes [14][15][16]19]. The shapes of rabbit erythrocytes were controlled by pH regulation to form spherocytes (swollen erythrocyte), echinocytes, enlarged erythrocytes (expanded discocytes similar to stomatocytes), and discocytes.…”

Section: Introductionmentioning

confidence: 55%

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Dielectric inspection of erythrocytes

Hayashi

Katsumoto

Oshige

et al. 2010

Journal of Non-Crystalline Solids

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Section: Introductionmentioning

confidence: 55%

“…where j l and j a are the limiting conductivities of the suspension and the supernatant fluid at low frequencies, respectively [14,16]. A suspension of spheroidal cells is also a system, for which dielectric responses are analytically solved (see [17,18] for details), and / is given by…”

Section: Introductionmentioning

confidence: 99%

Dielectric inspection of erythrocytes

Hayashi

Katsumoto

Oshige

et al. 2010

Journal of Non-Crystalline Solids

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“…frequency dependence), caused by the membrane of RBCs, in the radio frequency (RF) spectrum of the dielectric properties of blood [57]. This relaxation process is indentified as being of Maxwell-Wagner type [58,59] and termed, is known to increase with solute concentration [60], and has previously been observed in tissues [61].…”

Section: γ -Dispersionsmentioning

confidence: 99%

“…frequency dependence), caused by the membrane of RBC, was provided in the radio frequency (RF) spectrum of the dielectric properties of blood [12]. This relaxation process is indentified as being of Maxwell-Wagner type [59,60] and termed β -relaxation [60,61]. Most the theoretical models which describe the cell suspen-sions focus on β and γ -relaxations [17,14,61,62], including the often employed Pauly-Schwan model [63], them account for the non-spherical shape of cells.…”

Section: Dielectric Response and Erythrocyte Morphologymentioning

confidence: 99%

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Modeling of Dielectric Permittivity of the Erythrocytes Membrane as a Three-Layer Model

Batyuk¹,

Kizilova²

2018

Development Trends in Medical Science and Practice: The Experience of Countries of Eastern Europe and Prospects of Ukraine

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Abstract. The dielectric theories which used for applications of dielectric spectroscopy to investigate heterogeneous systems, such as particle suspensions, membranes, and tissue are described in this article. The dielectric constant ′ ε , the loss ′′ ε , and the conductivity σ of substances in a broad frequency range (1 Hz to 10 11 Hz), with for using a combination of different method of dielectric spectroscopy applied to identical samples was described. In the present work, we analyze the dispersion regions, commonly found in biological substances and tissue, usually as termed as α , β , δ and γ -dispersions. The nature of the dispersion caused by the membrane of RBCs in the radio frequency spectrum of the dielectric properties of blood which often is ascribed to the motion of bound water molecules is not yet fully understood. Dielectric properties of RBS membrane have been studied by means of the spheroidal model, where the three shells correspond to the double lipid bilayer and the spectrin network of the inner membrane, respectively. Based on the Maxwell-Wagner model and takes into account the morphological parameters of RBS membranes of cells using appropriate approximations the some simple relations was derived. In discussing the dielectric properties of RBS membrane was considered the mathematical model which accurately fit the experimental observations. Such models have obvious applications when characterizing the data and testing it against possible physical theories. Modeling of dielectric permittivity of the erythrocytes membrane as… IntroductionThe blood represents of heterogeneous material containing water, dissolved organic molecules, macromolecules, ions and soluble matter. The constituents of the blood are highly organized in cellular and subcellular structures forming macroscopic elements. The presence of ions in the blood plays an important role in the interaction with an electric field, providing means for ionic conduction and polarization effects. The ionic charge drift creates conduction currents and as knows as initiates polarization mechanisms through charge accumulation at structural interfaces, which occur at various organizational levels of membranes of cells of blood. Their dielectric properties will thus reflect contributions to the polarization from both structure and composition [1]. A common way to describe dielectric properties of cell of human blood or tissue between 1 Hz and 50 GHz is to fit ColeCole or Cole-Cole-type relaxation models to measurement data [2][3][4][5]. The measurement of dielectric properties of blood is known to be of importance for diagnosis of diseases [6][7][8][9][10][11]. Early studies of dielectric properties of the human erythrocytes are connecting with alternating current in the frequency domain were pioneered at the beginning of this century [12][13][14][15][16]. The frequency dependence of the dielectric properties of the RBC in suspension has been investigated by many scientists [17][18][19][20][21][22][23][24][25][26][27][28]. In the simplest model...

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Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

et al. 2017

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Whereas modern automated blood cell analyzers measure the volume of individual red blood cells (RBCs), leading to four RBC indices (mean corpuscular volume, MCV; mean corpuscular hemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; red cell distribution width, and RDW), the RBC shape has not been assessed by clinical screening tools. We applied the scanning flow cytometer (SFC) for complete characterization of intact RBC morphology in terms of diameter, maximal and minimal thicknesses, volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration, and content. The above-mentioned individual RBC characteristics were measured without fluorescent markers and other chemicals by a SFC equipped only with 660 nm laser for RBC illumination and single detector for measurement of angle-resolved light scattering. The distributions over all RBC characteristics were constructed and processed statistically to form the novel 31 RBC indices for 22 donor samples. Our results confirm the possibility of precise, label-free, enhanced morphological analysis of individual intact RBCs with compact single-detector flow cytometer. Detailed characterization of RBCs with high statistics and precision can be used to increase the value of screening examinations and to reveal pathologies accompanied by abnormality of RBC shape. © 2017 International Society for Advancement of Cytometry.

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Dielectric inspection of erythrocyte morphology

Cited by 48 publications

References 25 publications

Dielectric inspection of erythrocytes

Dielectric inspection of erythrocytes

Modeling of Dielectric Permittivity of the Erythrocytes Membrane as a Three-Layer Model

Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

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