Diazepinomicin, a New Antimicrobial Alkaloid from a Marine <i>Micromonospora</i> sp.

Charan, Romila D.; Schlingmann, Gerhard; Janso, Jeffrey E.; Bernan, Valerie S.; Feng, Xun; Carter, Guy T.

doi:10.1021/np040042r

Cited by 160 publications

(104 citation statements)

References 6 publications

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“…Currently, diazepinomicin is a unique farnesylated M.M.M. Raja et al (2016) Int J Appl Sci Biotechnol, Vol 4(4): 452-457 This paper can be downloaded online at http://ijasbt.org & http://nepjol.info/index.php/IJASBT dibenzodiazepinone and used as anticancer metabolite are derived from marine actinomycetes Micromonospora sp (Charan et al, 2004).The potential actinomyctes exhibited both intra and extracellular production of L-asparaginase by Streptomyces longsporusflavus (Abdel-Fattah and Olama,1998). Difference on production of L-asparaginase with yeast extract and peptone was observed and evaluated at 24h.…”

Section: Resultsmentioning

confidence: 99%

Comparative Studies on Effect of Carbon and Nitrogen Sources on L -Asparaginase Production

Raja¹,

A²,

Salique³

et al. 2017

Int J Appl Sci Biotechnol

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Marine actinomycetes sediment samples were collected from Gulf of Mannar costal region, Kayalpatinam, located at Tuticorin district, Tamilnadu, India. Marine actinomycetes were isolated and evaluated for activity of L-asparaginase production. A total of 10 marine actinomycetes strains were isolated. Among 10 isolates, six were belongs to Streptomyces sp, three were belongs to Micromonospora sp and one was to Micropolyspora sp. Based on phenotypic characteristics, actinomycetes strains were screened for L-asparaginase production. Streptomyces sp KPMS5 and Micromonospora sp KPMS10 were showed large pink coloration on L-asparaginase production medium. The strains were further studied for maximum production and characterizations of culture condition of L-asparaginase enzyme were evaluated. Effect of substrate on L-asparaginase production was evaluated by enzyme assy. Maximum enzyme assay (1.4 mM) was observed on glucose followed by starch (1.12Mm) by Micromonospora sp KPMS10. In Streptomyces sp KPMS5 showed maximum of 1.25mM of enzyme assay on glucose substrate followed by lactose 1.17mM. Yeast extract was effectively used as substrate for maximum production of L-asparaginase by submerged fermentation. Further studies on purification and characterization are required to support the application of enzyme. The finding concludes isolates belongs to non-Streptomyces sp like Micromonospora sp is a potential novel source for L-asparaginase production.

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Section: Resultsmentioning

confidence: 99%

Comparative Studies on Effect of Carbon and Nitrogen Sources on L -Asparaginase Production

Raja¹,

A²,

Salique³

et al. 2017

Int J Appl Sci Biotechnol

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“…Since environmental conditions of the sea are extremely different from terrestrial conditions, they produce different types of antibiotics. Several antibiotics have been isolated from marine actinobacteria by many researchers [20][21][22][23][24][25][26][27][28][29][30] . The isolated antibiotics are entirely new and unique when compared to those from the terrestrial ones 31 .…”

Section: Biotechnological Importance Of Marine Actinobacteriamentioning

confidence: 99%

Research on marine actinobacteria in India

et al. 2007

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Marine actinobacteriology is one of the major emerging areas of research in tropics. Marine actinobacteria occur on the sediments and in water and also other biomass (mangrove) and substrates (animal). These organisms are gaining importance not only for their taxonomic and ecological perspectives, but also for their unique metabolites and enzymes. Many earlier studies on these organisms were confi ned only to the temperate regions. In tropical environment, investigations on them have gained importance only in the last two decades. So far, from the Indian peninsula, 41 species of actinobacteria belonging to 8 genera have been recorded. The genus, Streptomyces of marine origin has been more frequently recorded. Of 9 maritime states of India, only 4 have been extensively covered for the study of marine actinobacteria. Most of the studies conducted pertain to isolation, identifi cation and maintenance of these organisms in different culture media. Further, attention has been focused on studying their antagonistic properties against different pathogens. Their biotechnological potentials are yet to be fully explored.

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“…In addition, the media also provides micro-elements as a sources of metals, such as ferrous iron and manganese. The growth patterns in this medium also bioactive compounds have been isolated from Micromonospora species, and have been shown to have antitumor, antiparasitic, and antimicrobe activity (Charan et al 2004;Igarashi et al 2007;Gärtner et al 2011;Sakai et al 2012). Other members of the genus Micromonospora that known as antibiotic producers were M. purpurea and M. echinospora, as gentamicin producers, and M. chalcea as a izumenolide producer.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Micromonospora spp. with Activity Against E.coli ATCC 35218 Resistance β-Lactam Antibiotics

2013

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Escherichia coli is a frequent cause of lifethreatening bloodstream infections and other common infections, such as urinary tract infections. Antibioticresistant E. coli are readily acquired via the diet (food and water), and other factors such as continuous drug usage, the in appropriate dose usage, and the natural factors of that microbe itself. The resistance results in increased mortality, morbidity, and health care cost. In Indonesia resistant E. coli have emerged, whereas the rates of resistance to some antibiotics were significantly higher, especially to beta-lactam classes (Lestari et al. 2008). Beta-lactams remain the most widely utilized antibiotics owing to their comparatively high effectiveness, low cost, and minimal side effects. Among the various classes of antibiotics, penicillin, and cephalosporins are the most frequently used agents in treatment of bacterial infection. The most common mechanism of resistance to this class of antibiotics is the ability of bacteria to produce beta-lactamases, enzymes that degrade or modify the antibiotic before it can reach the appropriate target site. These enzymes are very important for Gram-negative bacteria, including E. coli, as they constitute a major defense mechanism againstThe emerge of antibiotic resistance has been an important issue all over the world, on the other hand, infectious diseases have been one of the highest causes of death causes in the world. Therefore, the discovery of a new antimicrobial drug is very important, and the group of rare actinomycetes are really promising as the producer of new bioactive compounds, in this case antibiotics. In this study we screened and characterized the actinomycetes with antibacterial activity against Escherichia coli ATCC 35218 resistant beta-lactam antibiotics. A total of 96 strains collected in Biotechnology Microbial Culture Collection (BioMCC), BPPT, were screened for their antibacterial activities by the agar plug method. Three strains, at-HH-64, at-HH-78, and at-HH-259, showed antibacterial activity. The selected strains were cultured on four different media, both solid and liquid media, e.g. ISP2, ISP4, Micromonospora Starch Medium (MS), and Bennet's Medium (BM), and we characterized their morphology and growth patterns. Morphological characterization showed that all strains belonged to the genera Micromonospora. The active strains were also identified based on 16S rRNA partial sequence. BLAST search of the 16S rRNA sequences of all tested strains with the sequences available in the NCBI data bank showed a maximum similarity 99% with Micromonospora chersina.Key words : antibacterial activity, characterization, Micromonospora, screening Munculnya resistensi antibiotik telah menjadi isu penting di seluruh dunia, di sisi lain, penyakit menular telah menjadi salah satu penyebab kematian tertinggi di dunia. Oleh karena itu, penemuan obat antimikroba baru adalah sangat penting, dan kelompok aktinomiset langka benar-benar menjanjikan sebagai penghasil senyawa bioaktif baru, dalam hal ini antibiotik. Dalam ...

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Diazepinomicin, a New Antimicrobial Alkaloid from a Marine Micromonospora sp.

Cited by 160 publications

References 6 publications

Comparative Studies on Effect of Carbon and Nitrogen Sources on L -Asparaginase Production

Comparative Studies on Effect of Carbon and Nitrogen Sources on L -Asparaginase Production

Research on marine actinobacteria in India

Characterization of Micromonospora spp. with Activity Against E.coli ATCC 35218 Resistance β-Lactam Antibiotics

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