Characterization of Micromonospora spp. with Activity Against E.coli ATCC 35218 Resistance β-Lactam Antibiotics

Abstract

Escherichia coli is a frequent cause of lifethreatening bloodstream infections and other common infections, such as urinary tract infections. Antibioticresistant E. coli are readily acquired via the diet (food and water), and other factors such as continuous drug usage, the in appropriate dose usage, and the natural factors of that microbe itself. The resistance results in increased mortality, morbidity, and health care cost. In Indonesia resistant E. coli have emerged, whereas the rates of resistance to some antibiotics were significantly higher, especially to beta-lactam classes (Lestari et al. 2008). Beta-lactams remain the most widely utilized antibiotics owing to their comparatively high effectiveness, low cost, and minimal side effects. Among the various classes of antibiotics, penicillin, and cephalosporins are the most frequently used agents in treatment of bacterial infection. The most common mechanism of resistance to this class of antibiotics is the ability of bacteria to produce beta-lactamases, enzymes that degrade or modify the antibiotic before it can reach the appropriate target site. These enzymes are very important for Gram-negative bacteria, including E. coli, as they constitute a major defense mechanism againstThe emerge of antibiotic resistance has been an important issue all over the world, on the other hand, infectious diseases have been one of the highest causes of death causes in the world. Therefore, the discovery of a new antimicrobial drug is very important, and the group of rare actinomycetes are really promising as the producer of new bioactive compounds, in this case antibiotics. In this study we screened and characterized the actinomycetes with antibacterial activity against Escherichia coli ATCC 35218 resistant beta-lactam antibiotics. A total of 96 strains collected in Biotechnology Microbial Culture Collection (BioMCC), BPPT, were screened for their antibacterial activities by the agar plug method. Three strains, at-HH-64, at-HH-78, and at-HH-259, showed antibacterial activity. The selected strains were cultured on four different media, both solid and liquid media, e.g. ISP2, ISP4, Micromonospora Starch Medium (MS), and Bennet's Medium (BM), and we characterized their morphology and growth patterns. Morphological characterization showed that all strains belonged to the genera Micromonospora. The active strains were also identified based on 16S rRNA partial sequence. BLAST search of the 16S rRNA sequences of all tested strains with the sequences available in the NCBI data bank showed a maximum similarity 99% with Micromonospora chersina.Key words : antibacterial activity, characterization, Micromonospora, screening Munculnya resistensi antibiotik telah menjadi isu penting di seluruh dunia, di sisi lain, penyakit menular telah menjadi salah satu penyebab kematian tertinggi di dunia. Oleh karena itu, penemuan obat antimikroba baru adalah sangat penting, dan kelompok aktinomiset langka benar-benar menjanjikan sebagai penghasil senyawa bioaktif baru, dalam hal ini antibiotik. Dalam ...

Antibacterial Substance Produced by a Soil Bacteria Isolated From Rhizosphere of Zingiber Officinale

“…Approximately 17,000 compounds from those active compounds are antibiotics. A total of 17.6 % of antibiotics produced by bacteria, 52.7 % by Actinomycetales, and 29.7 % by fungi [2][3][4].…”

Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent

“…From 16500 antimicrobial compounds isolated from microbes, 52.7 % (8700 antimicrobials) were from Actinobacteria, while the remaining 29.7% and 17.6%, respectively, were isolated from fungi and bacteria (Bér-dy, 2005). Actinobacteria from tropical regions like Indonesia have also proved to have antimicrobial potency (Hidayati et al, 2013, Sipriyadi et al, 2016. Most of the reported data were based on actinobacteria with soil origin, while antimicrobial activity from endophytic actinobacteria is rarely reported.…”