Di(2-ethylhexyl)phthalate stimulates Ca2+ entry, chemotaxis and ROS production in human granulocytes

Exposure to Endocrine Disrupting Chemicals and Male Reproductive Health

“…Phthalates, mainly DEHP, DBP, or DEP, have been reported to alter the activities of marker testicular enzymes of laboratory animals associated with the specific events of spermatogenesis, inducing ROS production, lipid peroxidation, and apoptosis of spermatocytes. One possible mechanism is that ROS generation might correlate with DEHP-induced Ca 2+ entry, potentially through the Ca 2+ -mediated activation of the nicotinamide adenine dinucleotide phosphate (NADPH) complex (104). Recently, limited epidemiological studies have also reported that phthalate metabolites are associated with oxidative stress.…”

Increased urinary 8-hydroxy-2′-deoxyguanosine levels in workers exposed to di-(2-ethylhexyl) phthalate in a waste plastic recycling site in China

“…Experimental data indicated that DEHP might induce the productions of MDA and ROS (Palleschi et al 2009;Seo et al 2004) and subsequently cause oxidative DNA damage (Ito et al 2007;Seo et al 2004;Takagi et al 1990). In the present study, the greater occupational exposure to DEHP might have been a contributing factor to the elevated urinary 8-OHdG levels.…”

“…Recent studies found that DEHP is a nongenotoxic chemical, combination of the molecular signals and non-PPARα multiple pathways were involved in the hepatocarcinogenicity of DEHP (Ito and Nakajima 2008;Rusyn et al 2006). For instance, DEHP could stimulate the production of reactive oxygen species (ROS) in human granulocytes (Palleschi et al 2009), increase 8-hydroxy-2′-deoxyguanosine (8-OHdG), and malondialdehyde (MDA) levels in the livers of rodents exposed to DEHP (Ito et al 2007;Seo et al 2004). Chronic exposure to DEHP may induce oxidative stress and result in the accumulation of unrepaired deoxyribonucleic acid (DNA) damage (Maloney and Waxman 1999;Rusyn et al 2006) in the development of pulmonary, cardiovascular diseases, and cancer (Piconi et al 2003;Seitz and Stickel 2006).…”

Effects of Endocrine Disrupters on Immune Function and Inflammation

“…In mice, exposure to dibutyl phthalate was shown to alter the activity of macrophages, which affected their antigen presentation capacity [80]. Palleschi et al [81] found that human granulocytes were inappropriately activated following exposure to DEHP.…”