Dexketoprofen trometamol loaded solid lipid nanoparticles (SLNs): Formulation, in vitro and in vivo evaluation

Abstract

Development, in vitro and in vivo evaluation of dexketoprofen trometamol (DT)-loaded nanosized drug delivery system was aimed in this study. DT-loaded solid lipid nanoparticles (SLNs) were prepared using probe sonication. DT release from SLNs prepared and their kinetics were investigated. Structures of SLNs were elucidated by particle size and zeta potential measurements, shape and surface imaging, thermal analysis, X-ray diffraction, FT-IR and 1 H-NMR determinations. DT-loaded particles demonstrated characteristic plaque shapes while in vitro release studies showed extended release of DT. Korsmeyer-Peppas kinetic model was found to fit the best using DDSolver software program. Stability, cytotoxicity and in vivo animal experiments were further performed on DT-loaded SLN showing also prolonged analgesic activity in mice. Depending on the in vitro and in vivo test results, formulation developed in this study seems to prolong DT release and is promising for extending analgesic activity.

Dexketoprofen trometamol-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles: Preparation, <i>in vitro</i> characterization and cyctotoxity

“…Polydispersity index (PDI) indicates the range of particle size distribution, and PDI values in the range of 0 -1 demonstrate homogeneous distribution when it approaches zero. Very low PDI values of prepared formulation indicate homogeneous particle size distribution [13,20]. Zeta potential (ZP) values of PLGA NPs were found to be negative most probably due to the total negative charges of the functional groups on PLGA.…”

“…Higher CH 2 , CH 3 and CH signals in the copolymer caused those peaks to expand. Specific peaks of DT in 1 H-NMR spectrum were observed at 1.45 (d, 3H) ppm, 3.64 (s, 6H) ppm, 3.66 (q, 1IH) ppm and 7.41-7.80 (m, 9H) ppm [13].…”

“…It is a very simple and economic method [10]. Surveying extant patents and publications, some modified release formulations and locally available commercial preparations of DT were found [11][12][13].…”

Evaluation of carvedilol-loaded Eudragit® nanoparticles

“…Zeta potential value was found to be around +40 mV, indicating that the NPs have good stability. Higher Zeta potential is expected for the stable colloidal system as it overcomes the particle aggregation due to repulsion forces [24]. Positive surface charge of NPs were attributed to the cationic nature of Eudragit ® polymers [23,24].…”

“…Higher Zeta potential is expected for the stable colloidal system as it overcomes the particle aggregation due to repulsion forces [24]. Positive surface charge of NPs were attributed to the cationic nature of Eudragit ® polymers [23,24]. In vitro release profiles of the drugs can also be influenced by zeta potential of the NPs.…”