Devosia pacifica sp. nov., isolated from deep-sea sediment

Abstract

A novel bacterial strain, NH131 T , was isolated from deep-sea sediment of South China Sea. Cells were strictly aerobic, Gram-stain negative, short rod-shaped and motile with a single lateral flagellum. Strain NH131 T grew optimally at pH 6.5-7.0 and 25-30 6C. 16S rRNA gene sequence analysis revealed that strain NH131 T belonged to the genus Devosia, sharing the highest sequence similarity with the type strain, Devosia geojensis BD-c194 T (96.2 %). The predominant fatty acids were C 18 : 1 v7c, 11-methyl C 18 : 1 v7c, C 18 : 0 and C 16 : 0 . Ubiquinone 10 was the predominant ubiquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, three glycolipids and two unknown lipids. The DNA G+C content of strain NH131 T was 63.0 mol%. On the basis of the results of polyphasic identification, it is suggested that strain NH131 T represents a novel species of the genus Devosia for which the name Devosia pacifica sp. nov. is proposed. The type strain is NH131 T (5JCM 19305 T 5KCTC 32437 T ).The genus Devosia was first described by Nakagawa et al. (1996) as a result of the reclassification of 'Pseudomonas riboflavina' (Foster, 1944). The genus accommodates Gramstain negative, rod-shaped, aerobic, oxidase-positive bacteria containing ubiquinone 10 (Q-10) or Q-11 as the predominant respiratory quinone. The DNA G+C contents of bacteria of the genus Devosia range from 59.5 to 66.2 mol% (Yoon et al., 2007). At the time of writing, the genus Devosia comprises 15 species with validly published names, which can be found in the LPSN (Parte, 2014), and the species 'Devosia lucknowensis' (Dua et al., 2013) with a non-validly published name. In our attempts to study bacterial diversity of deep-sea sediments in the South China Sea, the marine strain NH131 T was isolated. In this paper, the classification of strain NH131 T by a polyphasic approach based on physiological, chemotaxonomic and phylogenetic analyses is described.Strain NH131 T was isolated from deep-sea sediment in the South China Sea. The sample of sediment was diluted with a tenfold dilution series method, spread on medium 702 (Nakagawa et al., 1996), which contained (per litre) 10.0 g peptone, 2.0 g yeast extract, 1.0 g MgSO 4 . 7H 2 O and 15 g agar (if needed) (pH 7.0), and incubated at 28 u C. After 3 days of aerobic incubation, one white colony, designated NH131 T , was picked. The colony was slightly convex, shiny and ranged from 0.5 to 1.0 mm in diameter with a complete rim.Cell morphology was examined by optical (BX40, Olympus) and transmission electron (JEM-1230, JEOL) microscopy using exponentially growing cells which were incubated in medium 702 for 24 h. The temperature range for growth of strain NH131 T was investigated on medium 702 at different temperatures (5-50 u C, in 5 u C increments) and the pH range was determined by adjusting the medium to pH 5.0-10.0 (in increments of 0.5 pH units) (Gomori, 1955). Growth under anaerobic conditions was determined after incubation of the novel strain in an anaerobic chamber on trypt...

Devosia nitraria sp. nov., a novel species isolated from the roots of Nitraria sibirica in China

“…The major fatty acid is C 16:0 (36.8%) with minor amounts of C 16:0 N alcohol (14.6%), 10-methyl C 17:0 (12.6%), C 18:1 ω 7 c (9.4%), C 18:0 (7.3%), C 19:0 cyclo ω8 c (5.5%), 11-Methyl C 18:1 ω7c (4.7%) and C 18:0 3-OH (2.5%) (Table 3). The cellular fatty acid profile was similar to the reference strains D. geojensis BD-c194 T (Ryu et al 2008) and D. pacifica NH131 T (Jia et al 2014) that have C 16:0 and C 18:1 ω 7 c as their main lipids, respectively. A large amount of unidentified glycolipid, diphosphatidylglycerol, phosphatidylglycerol and a small amount of unidentified polar lipids were present as polar lipids for strain 36-5-1 T (Fig.…”

Devosia marina sp. nov., isolated from deep seawater of the South China Sea, and reclassification of Devosia subaequoris as a later heterotypic synonym of Devosia soli

“…de/ genus/ devosia). Members of this genus are Gram-stain-negative, rod-shaped and non-spore-forming bacteria [2], and are ubiquitous in diverse ecological environments, such as soil [3], root nodules [4], alpine glacier cryoconite [5], beach and deep-sea sediments [6,7], and surface seawater [8], which clearly indicates their excellent environmental adaptation. Some isolates of the genus are capable of degrading deoxynivalenol and its derivatives [9] and of forming a unique nitrogen-fixing root-nodule symbiosis with the aquatic legume Neptunia natans (L.f.) Druce [10].…”

Seohaeicola zhoushanensis sp. nov., isolated from seawater

“…The polar lipids were extracted and separated by twodimensional TLC on silica gel plates (10Â10 cm; Merck 5554) (Minnikin et al, 1977). The solvent systems of the two dimensions were prepared as described by Jia et al (2014). The plates were then heated at 120 C for 10-15 min after spraying with 50 % (v/v) sulfuric acid ethanol solution.…”