Seohaeicola zhoushanensis sp. nov., isolated from seawater

Abstract

A novel, Gram-stain-negative, motile, facultatively anaerobic bacterium, designated strain NF48 T , was isolated from surface seawater around Zhoushan Islands. Cells were rod-shaped (1.1-3.9Â0.5-0.9 µm). Strain NF48T was able to grow at 10-40 C (optimum 30 C), at pH 5.5-9.0 (optimum 6.5-8.0) and with 0.5-7.0 % (w/v) NaCl (optimum 2.0 %). The genomic DNA G+C content was 65.5 mol%. Chemotaxonomic analysis showed that the main isoprenoid quinone was Q-10 and the major fatty acids were C 18 : 1 !7c and C 16 : 0 . The major polar lipids of strain NF48 T were phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NF48 T belonged to the genus Seohaeicola with similarity values of 95.5-97.2 % to members of this genus, and was most closely related to Seohaeicola nanhaiensis SS011A0-7#2-2 T (97.2 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain NF48