Developments in mycotoxin analysis: an update for 2011-2012

Abstract

This review highlights developments in mycotoxin analysis and sampling over a period between mid-2011 and mid- 2012. It covers the major mycotoxins aflatoxins, Alternaria toxins, ergot alkaloids, fumonisins, ochratoxin, patulin, trichothecenes, and zearalenone. A section on mycotoxins in botanicals and spices is also included. Methods for mycotoxin determination continue to be developed using a wide range of analytical systems ranging from rapid immunochemical-based methods to the latest advances in mass spectrometry. This review follows the format of previous reviews in this series (i.e. sections on individual mycotoxins), but due to the rapid spread and developments in the field of multimycotoxin methods by liquid chromatography-tandem mass spectrometry, a separate section has been devoted to advances in this area of research.

Survey of mycotoxins in Southern Brazilian wheat and evaluation of immunoassay methods

“…Considering that acetylates and conjugates are also toxicologically important, immunoassay results are useful for fast screening of DON in commodities. On the other hand, the ZEA levels estimated by immunoassay were comparable to those determined by UHPLC, agreeing with previous findings (Shephard et al, 2011).…”

“…Surveys on mycotoxins in commercial Brazilian wheat grain and byproducts provide critical information for growers to assess the impact of control measures as well as to inform consumers and policy makers on the mycotoxin risks (Furlong et al, 1995;Oliveira et al, 2002;Calori-Domingues et al, 2007;Del Ponte et al, 2012;Tralamazza et al, 2016). Among the quantitative methods for mycotoxin analyses, the most specific and sensitive ones include the ultra-high performance liquid chromatography-tandem with triple quadruple mass spectrometry (UHPLC-MS/MS), liquid chromatography coupled to mass spectrometry (LC-MS) and gas chromatography coupled to mass spectrometry (GC-MS) (Shephard et al, 2011). However, high costs are limiting factors for their use in routine for processing large number of samples (Lattanzio et al, 2009;Xu et al, 2010).…”

Comparison of ELISA, HPLC-FLD and HPLC-MS/MS Methods for Determination of Aflatoxin M1 in Natural Contaminated Milk Samples

“…Numerous studies in the recent years highlighted enzyme linked immunosorbent assay (ELISA) as the most frequently used technique for that purpose, followed by high performance liquid chromatography with fluorescence detector (HPLC-FLD) and liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). 18,19 Thin layer chromatography is almost abandoned as a technique since it is reliable for detection of AFM1 only for concentration above or around 0.05 μg/kg. 20 Further, recent studies have resulted in publication of a several biosensor-based methods for AFM1 determination in milk samples.…”

Mycotoxin Analysis: New Proposals for Sample Treatment

“…In this context, the use of robust analytical methodologies for sampling, sample treatment, and identification/quantification of mycotoxins in food and feed is mandatory, in order to protect the consumer health [10][11][12].…”