Development of New ‘Indigenous Dot-ELISA Kit’ as Sensitive Field Based Herd Screening Test for the Diagnosis of Johne’s Disease in the Domestic Buffalo Population

Molecular and Serological Detection of Mycobacterium avium Subspecies Paratuberculosis in Ruminants of Jabalpur Region

“…It is a powerful alternative diagnostic method for bacterial culture by which specific amplification of MAP DNA can be done (Biswal et al, 2020 andSingh et al, 2020). The IS900 gene is an ideal target for direct faecal and tissue PCR with high sensitivity as greater number of copies of this gene are present in Map genome than other genes like ISMav2, ISMap02 or F57 (Kaur et al, 2011;Singh et al, 2013 andHassan et al, 2019). Therefore, in the present study, suspected faecal and tissue samples were screened using IS900 PCR on extracted DNA to obtain the frequency of MAP distribution in goats, buffaloes and cattle.…”

Antibodies against Mycobacterium avium subspecies paratuberculosis in Cattle of Indore District in Madhya Pradesh

“…Paratuberculosis is diagnosed tentatively clinically (history of chronic diarrhoea that is generally untreatable and extreme emaciation in affected bovines) and also definitively by laboratory diagnostic methods such as acid fast staining (AFS) of faecal material and further confirmation by agent isolation from faeces of infected animal, serological testing by using agar gel immunodiffusion (AGID), complement fixation test (CFT), Enzyme linked immunosorbent assay (ELISA), fluorescent antibody technique (FAT), dot-ELISA and molecular methods such as polymerase chain reaction (PCR). Rectal biopsy or scraping and lymph node biopsy followed by AFS, intradermal johnin and intravenous johnin testing are also conducted for the diagnosis (Singh et al, 2016b;Chakrabarti, 2003). In the present investigation, indigenously developed ELISA is used for screening serum samples of cattle to evaluate antibody response against MAP infection.…”