Profiling of Mycobacterium avium subspecies paratuberculosis in the milk of lactating goats using antigen-antibody based assays

Singh, Manju; Gupta, Saurabh; Chaubey, Kundan Kumar; Singh, Shoor Vir; Sohal, Jagdip Singh

doi:10.1016/j.cimid.2019.02.006

Cited by 3 publications

(2 citation statements)

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“…Lactogenic shedding of MAP is assumed to be discontinuous as has been shown for fecal shedding [ 24 , 25 ]. During the dry period, MAP is possibly transferred within macrophages from the intestine over the lymphatic system to the udder.…”

Section: Discussionmentioning

confidence: 99%

“…In a study from The Netherlands, MAP could not be detected in any colostrum sample from animals in the subclinical stage of disease, whereas it was detected in four percent of the milk samples obtained later during lactation [ 18 ]. MAP was also detected in milk samples from dairy goats during different timepoints of lactation in a study from India [ 25 ]. Although the number of relevant studies is very small, there seems to be a genus difference which cannot be explained at the moment.…”

Section: Discussionmentioning

confidence: 99%

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Analysis of Colostrum and Udder Skin Swabs from a Dairy Goat Herd in Germany regarding the Occurrence of Mycobacterium avium Subsp. paratuberculosis

Pickrodt

Donat

Moog³

et al. 2022

Animals

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Oral intake of Mycobacterium avium subspecies paratuberculosis (MAP) in first days of life is considered to be the main route of infection for paratuberculosis. This can be related to a direct contact to contaminated feces or feeding of MAP containing colostrum. Colostrum is believed to become contaminated either by lactogenic shedding or introduction of MAP from environmental sources. In this pilot study, the presence of MAP in individual and bulk colostrum samples from a paratuberculosis-infected, vaccinated dairy goat herd in Germany and the effect of udder skin disinfection on the MAP load of colostrum were examined. In order to distinguish between lactogenic shedding and fecal contamination, 49 udder skin swabs were cultivated on solid medium whereas 29 swabs were additionally analyzed by qPCR. qPCR was applied on 110 individual colostrum samples collected from 55 goats, one before and one after disinfection with a mycobactericidal disinfectant, and 14 bulk colostrum samples. MAP DNA was detected in 10.3% (3/29) of the swab samples, but no viable MAP was cultivated from any sample. These results indicate a low-level MAP contamination of the udder skin and colostrum of milking goats suggesting a low risk of MAP transmission via these routes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%