Development of interspecific F1 hybrids (Solanum melongena × Solanum khasianum) in eggplant through embryo rescue technique

Rattan, Puja; Kumar, Sanjeev; Salgotra, Romesh Kumar; Samnotra, R. K.; Sharma, F.L.

doi:10.1007/s11240-014-0591-4

Cited by 17 publications

(4 citation statements)

References 14 publications

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“…S. aculeatissimum , a wild relative of cultivated eggplant, is a well-known economically important plant, and is widely cultivated as a medicinal crop, possessing some desirable characteristics, especially the resistance to soil-borne diseases such as verticillium wilt and root-knot nematode [ 13 , 18 , 19 , 20 ]. The successful hybridization between S. aculeatissimum and S. melongena provides the opportunity to utilize resistance genes from S. aculeatissimum [ 21 , 22 ]. In a previous study, we obtained a partial NBS-LRR class resistance gene sequence from transcriptome sequencing of S. aculeatissimum , which had a high similarity with Mi-1 gene of tomato [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Characterization of a Wild Eggplant Solanum aculeatissimum NBS-LRR Gene, Involved in Plant Resistance to Meloidogyne incognita

Zhou

Liu

Bao

et al. 2018

IJMS

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Root-knot nematodes, Meloidogyne spp., cause considerable damage in eggplant production. Transferring of resistance genes from wild relatives would be valuable for the continued improvement of eggplant. Solanum aculeatissimum, a wild relative of eggplant possessing resistance to Meloidogyne incognita, is potentially useful for genetically enhancing eggplant. In the present study, we have isolated and characterized a nucleotide-binding site leucine-rich repeat (NBS-LRR) resistance gene, designated as SacMi. The full-length cDNA of the SacMi gene was obtained using the technique of rapid-amplification of cDNA ends (RACE). The open reading frame of the SacMi gene was 4014 bp and encoded a protein of 1338 amino acids. Sequence analysis indicated that SacMi belong to the non- Toll/Interleukin-1 receptor (TIR)-NBS-LRR type disease-resistance genes. Interestingly, quantitative RT-PCR showed that SacMi is expressed at low levels in uninfected roots, but was up-regulated by infection with M. incognita. To investigate the role of SacMi in S. aculeatissimum resistance against M. incognica, the tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) system was used. Silencing of SacMi enhanced susceptibility of S. aculeatissimum plants to M. incognita, suggesting the possible involvement of SacMi in resistance against M. incognita infection.

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Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Characterization of a Wild Eggplant Solanum aculeatissimum NBS-LRR Gene, Involved in Plant Resistance to Meloidogyne incognita

Zhou

Liu

Bao

et al. 2018

IJMS

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“…Previous attempts such as in-vitro selection, embryo rescue, somatic hybridization and genetic engineering have greatly improved the varieties of eggplant ( Swamynathan et al . 2010 ; Rattan et al . 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Shoot Induction in White Eggplant (Solanum melongena L. Cv. Bulat Putih) using 6-Benzylaminopurine and Kinetin

Foo

Lee

Chin

et al. 2018

TLSR

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Solanum melongena L. commonly known as the eggplant or brinjal comes from the family of Solanaceae, sharing the same ancestor with the tomato and potato. It is an economically important crop worldwide, being well studied for its medicinal properties, nutritional values and its role as an alternative model plant. The eggplant fruit has been previously used for treatments of various diseases such as bronchitis, asthma, arthritis and diabetes as well as its nutritive properties that are beneficial to the human diet. Plant transformation studies on the eggplant have been widely done for the production of transgenic eggplants harbouring genes that are beneficial for optimal plant growth and fruit production. Shoot induction is an essential step required for the successful regeneration of transformed plant tissues and therefore is an essential pre-requisite in Agrobacterium-mediated transformation. The local eggplant cv. Bulat Putih is a local cultivar of eggplant in Malaysia with white and round fruits making it a potential model plant colour pigment accumulation studies in fruit crops. The current work aims to investigate the shoot induction potential of 6-benzylaminopurine (BAP) and Kinetin from cotyledon explants of eggplant cv. Bulat Putih. Results indicated that both BAP and Kinetin were able to induce the regeneration of callus from cotyledon explants. On the other hand, Kinetin at the concentration of 2.0 mg/L successfully induced shoots at the value of 1.50 ± 0.22 shoots per explant, whereas BAP alone did not trigger any formation of shoots. This study indicated that kinetin alone is sufficient to induce shoots in eggplant cv. Bulat Putih without the presence of BAP.

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“…Embryos are removed from the ovary after fertilization but before abortion, and cultured aseptically on suitable medium to generate viable plants. This has been applied, for example, to interspecific hybrids: S. melongena × S. torvum (Kumchai et al, 2013), S. melongena × S. khasianum (Rattan et al, 2015) and Capsicum annuum × Capsicum baccatum (Jae et al 2006;Eggink et al, 2014), although further de velopment of embryorescue techniques in Capsicum peppers is needed (Manzur et al, 2013). In the case of S. lycopersicum × Solanum peruvianum L., the development of lines carrying resistance to tomato yellow leaf curl virus, tomato spotted wilt virus and to mato chlorosis virus was possible (Picó et al, 2002;Encina et al, 2012;Julián et al, 2013).…”

Section: Interspecific Hybrids and Hybridization Barriersmentioning

confidence: 99%

Rootstock breeding: current practices and future technologies.

Thompson

Picó²,

Yetışır³

et al. 2017

Vegetable Grafting: Principles and Practices

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This chapter explores how genetic diversity can be exploited more deeply to create new rootstock cultivars, and describes the current and future breeding strategies applicable to rootstocks. It considers how to combine different sources of genetic variation, for example by overcoming species barriers, and how to select useful rootstocks. The many traits that can be associated with the root system or conferred by a rootstock to the scion are outlined. The impact of the practice of grafting on breeding strategies is also discussed.

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Development of interspecific F1 hybrids (Solanum melongena × Solanum khasianum) in eggplant through embryo rescue technique

Cited by 17 publications

References 14 publications

Molecular Cloning and Characterization of a Wild Eggplant Solanum aculeatissimum NBS-LRR Gene, Involved in Plant Resistance to Meloidogyne incognita

Molecular Cloning and Characterization of a Wild Eggplant Solanum aculeatissimum NBS-LRR Gene, Involved in Plant Resistance to Meloidogyne incognita

Shoot Induction in White Eggplant (Solanum melongena L. Cv. Bulat Putih) using 6-Benzylaminopurine and Kinetin

Rootstock breeding: current practices and future technologies.

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