Molecular Cloning and Characterization of a Wild Eggplant Solanum aculeatissimum NBS-LRR Gene, Involved in Plant Resistance to Meloidogyne incognita

Zhou, Xiaohui; Liu, Jun; Bao, Shengyou; Yang, Yan; Zhuang, Yun

doi:10.3390/ijms19020583

Cited by 31 publications

(20 citation statements)

References 50 publications

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“…Our results indicated that ZmNBS25 was expressed at relatively low levels in uninfected maize leaves; however, it was significantly upregulated at 24 h post B. maydis inoculation, indicating that ZmNBS25 might be associated with B. maydis resistance in maize. Similar expression patterns have been observed in other plants NBS-LRR genes, such as: AhRRS5 , a R. solanacearum resistance gene in peanut ( Zhang et al, 2017 ); Xa1 , a bacterial resistance gene from rice ( Yoshimura et al, 1998 ); and SacMi , a M. incognita resistance gene ( Zhou et al, 2018 ). In addition, the expression level of ZmNBS25 was significantly upregulated by SA treatment.…”

Section: Discussionsupporting

confidence: 72%

The Maize NBS-LRR Gene ZmNBS25 Enhances Disease Resistance in Rice and Arabidopsis

Xu¹,

Liu²,

Zhu³

et al. 2018

Front. Plant Sci.

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Nucleotide-binding site-leucine-rich repeat (NBS-LRR) domain proteins are immune sensors and play critical roles in plant disease resistance. In this study, we cloned and characterized a novel NBS-LRR gene ZmNBS25 in maize. We found that ZmNBS25 could response to pathogen inoculation and salicylic acid (SA) treatment in maize, and transient overexpression of ZmNBS25 induced a hypersensitive response in tobacco. High-performance liquid chromatography (HPLC) analysis showed that, compared to control plants, ZmNBS25 overexpression (ZmNBS25-OE) in Arabidopsis and rice resulted in higher SA levels. By triggering the expression of certain defense-responsive genes, ZmNBS25-OE enhanced the resistance of Arabidopsis and rice to Pseudomonas syringae pv. tomato DC3000 and sheath blight disease, respectively. Moreover, we found little change of grain size and 1000-grain weight between ZmNBS25-OE rice lines and controls. Together, our results suggest that ZmNBS25 can function as a disease resistance gene across different species, being a valuable candidate for engineering resistance in breeding programs.

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Section: Discussionsupporting

confidence: 72%

The Maize NBS-LRR Gene ZmNBS25 Enhances Disease Resistance in Rice and Arabidopsis

Xu¹,

Liu²,

Zhu³

et al. 2018

Front. Plant Sci.

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“…Eggplant is sensitive to cold stress and often undergoes the cold stress during its growth and development, especially in the early spring (Zhou et al, 2018a;Zhou et al, 2018b). Therefore, it is important to explore the mechanism for cold tolerance and how to improve this tolerance.…”

Section: The Differentially Expressed Smwrkys In Response To Cold Stressmentioning

confidence: 99%

“…VIGS was an effective tool to study the gene functions (Zhang et al, 2018;Xu et al, 2019). VIGS mediated by TRV has been successfully employed in the functional analysis of eggplant genes (Zhou et al, 2018a;Zhou et al, 2018b). We conducted a functional validation for SmWRKY26 and SmWRKY32 using VIGS.…”

Section: Silencing Smwrky26 and Smwrky32 Increased The Sensitivity Tomentioning

confidence: 99%

“…The information of eggplant genome has been released in 2014 (Hirakawa et al, 2014). Several gene families were identified, such as CBL and CIPK genes (Li et al, 2016), R2R3MYB transcription factor superfamily (Wang et al, 2016), calcium-dependent protein kinases (CDPKs) (Kumari et al, 2017) and CBF genes (Zhou et al, 2018a;Zhou et al, 2018b). Previously, Yang et al (2015) have identified the WRKY proteins in Solanum melongena L. and Solanum torvum Sw. using the RNA-sequencing data, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Identification of WRKY gene family and characterization of cold stress-responsive WRKY genes in eggplant

Yang

Zhou

et al. 2020

PeerJ

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Background WRKY proteins play a vital role in the plants response to different stresses, growth and development. Studies of WRKY proteins have been mainly focused on model plant Arabidopsis and a few other vegetable plants. However, the systematical study of eggplant WRKY transcription factor superfamily is scarce. Methods Bioinformatics has been used to identify and characterize the eggplant WRKY gene family. For the exploration of the differentially expressed WRKY genes, two cultivars with different cold-tolerance were used. Finally, we performed a virus-induced gene silencing (VIGS) experiment to verify the functions of SmWRKY26 and SmWRKY32. Results Fifty eight (58) genes encoding eggplant WRKY proteins were identified through searching the eggplant genome. Eggplant WRKY proteins could be classified into three groups or seven subgroups in accordance with other plants. WRKY variants were identified from the eggplant. Gene structure analysis showed that the number of intron in eggplant WRKY family was from 0 to 11, with an average of 4.4. Conserved motif analysis suggested that WRKY DNA-binding domain was conserved in eggplant WRKY proteins. Furthermore, RNA-seq data showed that WRKY genes were differentially expressed in eggplant response to cold stress. By using VIGS, the two differentially expressed genes-SmWRKY26 and SmWRKY32 were verified in response to cold stress. Discussions This study provides a foundation for further exploring the functions of WRKY proteins in eggplant response to stresses and eggplant genetic improvement in stresses.

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“…For example, a study identified a resistance gene—a nucleotide-binding site leucine-rich repeat (NBS-LRR)—against the root-knot nematode Meloidogyne spp. from the wild relative of eggplant, Solanum aculeatissimum [14], and discussed the biotechnological potential of transferring the gene to cultivated eggplants to create more resistant introgression lines. Several other examples exist in the literature.…”

Section: Introductionmentioning

confidence: 99%

Agrobiotechnology Goes Wild: Ancient Local Varieties as Sources of Bioactives

Berni

Cantini

Romi

et al. 2018

IJMS

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The identification and use of species that have best adapted to their growth territory is of paramount importance to preserve biodiversity while promoting sustainable agricultural practices. Parameters including resistance to natural conditions (biotic and abiotic risk factors), biomass and fruit productivity, and phytochemical content with nutraceutical potential, could be used as quantitative markers of the adaptability of plants to wild environments characterized by minimal human impact. Ancient varieties, which are plant varieties growing in regional territories and not destined for market distribution, are a source of unique genetic characters derived from many years of adaptation to the original territory. These plants are often more resistant to biotic and abiotic stresses. In addition, these varieties have a high phytochemical (also known as bioactives) content considered health-beneficial. Notably, the content of these compounds is often lower in commercial cultivars. The use of selected territorial varieties according to the cultivation area represents an opportunity in the agricultural sector in terms of biodiversity preservation, environmental sustainability, and valorization of the final products. Our survey highlights the nutraceutical potential of ancient local varieties and stresses the importance of holistic studies (-omics) to investigate their physiology and secondary metabolism.

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Molecular Cloning and Characterization of a Wild Eggplant Solanum aculeatissimum NBS-LRR Gene, Involved in Plant Resistance to Meloidogyne incognita

Cited by 31 publications

References 50 publications

The Maize NBS-LRR Gene ZmNBS25 Enhances Disease Resistance in Rice and Arabidopsis

The Maize NBS-LRR Gene ZmNBS25 Enhances Disease Resistance in Rice and Arabidopsis

Identification of WRKY gene family and characterization of cold stress-responsive WRKY genes in eggplant

Agrobiotechnology Goes Wild: Ancient Local Varieties as Sources of Bioactives

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