Development of electrosprayed mucoadhesive chitosan microparticles

Moreno, Jorge Alberto Sevilla; Mendes, Ana Carina Loureiro; Stephansen, Karen Boutrup; Engwer, Christoph; Goycoolea, Francisco M.; Boisen, Anja; Chronakis, Ioannis S.

doi:10.1016/j.carbpol.2018.02.062

Cited by 82 publications

(46 citation statements)

References 38 publications

(28 reference statements)

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“…When utilizing electrospray for the preparation of the chitosan particles optimization is needed. Previously, the three chitosan types of HMC + (70, 85 and 90) have been optimized in terms of forming chitosan stable microparticles (24). It has been an area of focus to investigate which of the three chitosan types would be most suited for the purpose of oral vaccination.…”

Section: Results and Discussion Morphology Size And Zeta Potentialmentioning

confidence: 99%

“…For the preparation of the chitosan particles with OVA and Quil-A, a similar method was used as described in the literature (24), but in this study with the addition of antigen and adjuvant to the particles. Here, 150 mg of OVA and 15 mg of Quil-A were diluted in 4.5 mL of water, then 0.3 g of chitosan was dispersed, followed by the addition of 0.5 mL of AA in the case of HMC + 70/5 and HMC + 85/5, while for HMC + 90/10, 3.5 mL of water and 1.5 mL of AA were added.…”

Section: Preparation Of Solutions For Electrospraying Chitosan Particlesmentioning

confidence: 99%

“…In our previous study, chitosan electrosprayed microparticles were produced and optimized for oral drug delivery (24). Chitosan´s with different molecular weight, degree of deacetylation (DD) and polymerization (DP), together with the different solvent compositions were investigated.…”

Section: Introductionmentioning

confidence: 99%

“…It was found that stable chitosan particles in aqueous solutions with average size of approximately 1 µm were produced by dissolving 3 % w/v of low molecular weight chitosan in mixtures of aqueous acetic acid and ethanol (50/50 % v/v). Furthermore, these microparticles had good mucoadhesive properties showing its potential as a drug delivery carrier for this application of oral vaccine delivery (24). The aim of this paper was to develop chitosan microparticles loaded with the subunit model antigen, OVA and the adjuvant, Quil-A using electrospraying as the encapsulation technique.…”

Section: Introductionmentioning

confidence: 99%

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Preparation and Characterization of an Oral Vaccine Formulation Using Electrosprayed Chitosan Microparticles

et al. 2018

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Chitosan particles loaded with the antigen ovalbumin (OVA) and the adjuvant, Quil-A were produced by electrospray, using mixtures of water/ethanol/acetic acid as a solvent. Three different chitosan's designed as HMC + 70, HMC + 85 and HMC + 90 (designated as 705010, 855010 and 905010) were tested and its efficacy to be used in oral vaccine delivery applications was investigated. The morphology, size and zeta potential of the produced particles were investigated, together with the encapsulation efficiency and release of OVA from the three chitosan formulations. Moreover, the mucoadhesion and cytotoxicity the chitosan microparticles was examined. All the three formulations with OVA and Quil-A were in the micrometer size range and had a positive zeta potential between 46 to 75 mV. Furthermore, all the three formulations displayed encapsulation efficiencies above 80 % and the release of OVA over a period of 80 h was observed to be between 38-47 %. None of the developed formulations exhibited high mucoadhesive properties, either cytotoxicity. The formulation prepared with HMC + 70, OVA and Quil-A had the highest stability within 2 h in buffer solution, as measured by dynamic light scattering. The electrosprayed formulation consisting of HMC + 70 with OVA and Quil-A showed to be the most promising as an oral vaccine system.

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Section: Results and Discussion Morphology Size And Zeta Potentialmentioning

confidence: 99%

Section: Preparation Of Solutions For Electrospraying Chitosan Particlesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Preparation and Characterization of an Oral Vaccine Formulation Using Electrosprayed Chitosan Microparticles

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“…1 The known characteristics of chitosan are dependent on multiple items: deacetylation degree, distribution of acetyl groups within the chain, and the polysaccharide molecular weight. 2 Chitosan, which is composed of glucosamine and N-acetyl glucosamine, 3 has been reported to have multiple bioactivities such as reducing cholesterol levels, 4 involving the hemostatic processes, 5 accelerating wound healing, 6 and inhibiting tumor growth. 7 Chitosan is considered a useful compound in the biomedical, food, biotechnology, and pharmaceutical fields due to its remarkable features, which include biocompatibility, biodegradability, and safety.…”

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confidence: 99%

Chitosan is a potential inhibitor of ovarian cancer: Molecular aspects

et al. 2019

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Although ovarian cancer has a lower prevalence than breast cancer, its mortality rate is three times higher, which is reported to increase in the coming years. As the early stages of ovarian cancer do not have any obvious symptoms, in most of the cases, this cancer is diagnosed at advanced stages with a poor prognosis. Moreover, in many patients who are diagnosed with advanced stage, relapse of the disease and drug resistance are observed. Over the past years, these women have been treated with chemotherapy and cytoreductive surgeries. However, the chemotherapy could affect the healthy tissues in addition to the malignancies. Therefore, discovering new diagnostic and therapeutic options seems to be a crucial need. Unlike the common invasive and/or nonspecific treatments, nanomedicine is trying to find a new way for cancer imaging, diagnosis, and drug delivery method. Nanoparticles (NPs), which has recently drawn attention, can be used in order to reduce the toxicity and frequent dosing of drugs, tumor‐specific delivery, and early diagnosis for malignancies. Chitosan as an NP and product of chitin deacetylation has multiple characteristics, including biocompatibility, biodegradability, and safety. In this review, we cover the studies concerned with the role of chitosan in finding solutions to overcome the problems faced in ovarian cancer treatments. Furthermore, we highlight how chitosan is being used in delivering chemotherapy drugs, gene therapy, and imaging methods for both detection and image‐guided therapies.

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