Development of an Intra-oral Single-section Remineralization Model

Wefel, James S.; Maharry, G. J.; Jensen, Mark E.; Harless, J.D.

doi:10.1177/00220345870660091401

Cited by 41 publications

(20 citation statements)

References 12 publications

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“…1986;Wcfel et al, 1987] sec tions were used rather than bulk enamel, to allow each le sion to act as its own control, thus overcoming problems caused by biological variation. In addition, special care was undertaken to ensure that the same part o f each lesion was measured on every occasion, thereby improving reproduc ibility, and, as mineralization is influenced by Az [Strang et al, 1987;Schafer et al.…”

Section: Discussionmentioning

confidence: 99%

Demonstration of a Fluoride Dose Response with an In situ Single-Section Dental Caries Model

Damato¹,

Stephen²

1994

Caries Res

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The in situ dental caries model developed in Glasgow was evaluated by determining whether it could demonstrate a fluoride dose response in a crossover study using 0, 250, and 1,000 ppm F from sodium fluoride dentifrices. In situ appliances, each carrying four enamel sections with multiple artifícal carious lesions, were worn by 12 dentate subjects for three 5-week experimental runs, during which they brushed twice daily with a test paste. Mineral changes in the lesions were measured by analyzing microradiographs, using an image analysis system, after 0, 2, 4, and 5 weeks and the rate of change calculated. Two-way analysis of variance showed no significant intersubject effects (for any of the parameters), but demonstrated strong evidence of a linear fluoride dose effect: total mineral loss (▵z) p = 0.0596; lesion body p = 0.0423; surface zone p = 0.0081. However, the exclusion of the data from 1 low-caries (DMFS = 1), high-calculus-forming subject produced even stronger evidence as to the efficacy of this model (▵z p = 0.0226; lesion body p = 0.0164; surface zone p = 0.0081). Hence, these results suggest this model is useful for studying fluoride products according to guidelines of American Dental Association.

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Section: Discussionmentioning

confidence: 99%

Demonstration of a Fluoride Dose Response with an In situ Single-Section Dental Caries Model

Damato¹,

Stephen²

1994

Caries Res

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“…The single-section intraoral crown model allows one to evaluate the de-and remineralization effects of foods, fluoride rinses, dentifrices, and other oral care products in a shorter period of time and at a lower cost than is normally associated with a longterm clinical trial [Wefel et al, 1987]. In this study, extracted, caries-free, human third molars were cleaned of all debris and covered with an acid-resistant varnish leaving a standardized 1 !…”

Section: Single-section Crown Modelmentioning

confidence: 99%

Effect of an Alcohol-Free, 1% Chlorhexidine Gel as an Adjunct to a Fluoridated Dentifrice Using an Intraoral Crown Model

et al. 2007

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Background/Aims: The use of chlorhexidine as a topically applied oral antiseptic is well documented; however, clinical studies examining the effects of chlorhexidine gel on in situ dental caries are limited. This study utilized an in situ caries model and a modified crossover design to examine whether the addition of a biweekly topical, alcohol-free, 1% chlorhexidine digluconate gel to a daily fluoridated dentifrice inhibited artificial caries in dental tissues better than the fluoridated dentifrice alone when compared to a nonfluoridated placebo dentifrice. Methods: Thirty patients were recruited based on their need for a mandibular, full crown. Artificial caries lesions were created in extracted human teeth and enamel and root tissue sections 100 µm in thickness were characterized using polarized light microscopy. The sections were fixed in the crown and placed on the prepared tooth. The participants were assigned a placebo toothpaste, a toothpaste with 1,100 ppm F or a 1,100 ppm F toothpaste followed by 1 ml of 1% chlorhexidine gel at day 1 and day 14 (chlorhexidine+). Patients were instructed to brush twice daily for 4 weeks. Following each round, the sections in the crown were replaced with new sections. The sections were recharacterized and the mean changes were compared using ANOVA at α = 0.05. Results: The chlorhexidine + Fdentifrice and the F dentifrice alone significantly reduced lesion area in enamel tissue when compared to the placebo dentifrice. Both treatments also inhibited lesion progression and initiation in root tissue better than control in this model system. Although the chlorhexidine+ group enhanced remineralization and inhibited lesion progression better than the F^– dentifrice alone for all outcomes measured, the differences were not significant. Conclusions: The chlorhexidine, in conjunction with a fluoride dentifrice, was no more effective than the fluoride dentifrice alone. Further study is needed before this 1% alcohol-free chlorhexidine gel should be recommended as an adjunct to a fluoride dentifrice in the treatment of dental caries.

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“…Several authors [von Bartheld, 1980;Featherstone and Silverstone, 1982;Harvey et al, 1982;ten Cate and Exterkate, 1986;Mellberg et al, 1986;Creanor et al, 1986;Wefel et al, 1987;Strang et al, 1987;1988;Stephen et al, 1992;Lammers et al, 1990;Exterkate et al, 1993;Damato and Stephen, 1994] employed various varnishes and adhesives to prepare 'windows' and/or to cover cut surfaces. To 398 Caries Res 1998;32:393-400 Iijima/Takagi/Duschner/Ruben/Arends the authors' knowledge no previous work on the importance of NV penetration has been reported.…”

Section: Nv Penetrationmentioning

confidence: 99%

“…Single-section techniques have been used to investigate demineralization and remineralization of enamel and dentine in vitro [Featherstone and Silverstone, 1982;Harvey et al, 1982;ten Cate and Exterkate, 1986;Strang et al, 1988;Lammers et al, 1990;Exterkate et al, 1993;Inaba et al, 1995] as well as in situ [Creanor et al, 1986;Mellberg et al, 1986Mellberg et al, , 1992Mellberg, 1992;Wefel et al, 1987;Wefel, 1990;Strang et al, 1987;Stephen et al, 1992;Damato and Stephen, 1994]. Two types of single-section techniques are currently used to create lesions in enamel or dentine.…”

mentioning

confidence: 99%

“…Another aspect when thin sections are employed is the coating or sealing of exposed section sides. Nail varnish (NV) is most widely used to protect cut surfaces of thin sections in a demineralization or remineralization experiment [Creanor et al, 1986;ten Cate and Exterkate, 1986;Strang et al, 1988;Wefel et al, 1987;Stephen et al, 1992;Damato and Stephen, 1994].…”

mentioning

confidence: 99%

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Influence of Nail Varnish on the Remineralization of Enamel Single Sections Assessed by Microradiography and Confocal Laser Scanning Microscopy

Iijima

Takagi²,

Duschner³

et al. 1998

Caries Res

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Single–section techniques are attractive in enamel de– and remineralization investigations because they allow longitudinal studies in which mineral changes can be assessed by microradiography (TMR). Nail varnish (NV) is in general applied to coat the cut thin–section sides. The aims of this study were to investigate: (1) NV penetration depth in cut surfaces of demineralized enamel, (2) the influence of NV on cut surfaces of demineralized enamel on TMR, (3) the influence of NV penetration on a following remineralization. Cut surfaces of thin sections of demineralized enamel were NV coated; the NV was peeled off and the penetration depth assessed by confocal laser scanning microscopy. The NV penetration was 18±5 μm (mean±SD) in demineralized enamel. To evaluate the possible influence of NV on TMR, cut surfaces of thin sections of demineralized enamel were coated (twice) and microradiographed before and after nail varnishing. The NV effect (total effect of penetrated and surface NV) on the main parameters of TMR, Ld and ΔZ, was less than 5% of the mean values. In the remineralization experiment (remineralization with 1.5 mM Ca²⁺, 0.9 mM phosphate, pH 7, 1 ppm F for 1 and 2 weeks), lesions in bulk samples, lesions in thin sections with NV–coated cut surfaces and lesions in thin sections in a PMMA (polymethylmethacrylate) holder were compared. (1) The remineralization of bulk samples and of NV–coated thin sections is different in one aspect. The amounts of mineral deposited in the lesions expressed as ΔZ are comparable after 1 week. But because the NV penetrates part of the lesion outside, there was an Ld difference. The lesion depth difference between bulk lesions and NV–coated lesions in thin sections was statistically significant and was about 19% less in NV–coated lesions after 1 week; after 2 weeks of remineralization there was no difference in Ld between bulk– and NV–coated lesions any more. (2) There was no difference in remineralization efficacy between lesions in bulk samples and lesions in thin sections in the PMMA holder.

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Development of an Intra-oral Single-section Remineralization Model

Cited by 41 publications

References 12 publications

Demonstration of a Fluoride Dose Response with an In situ Single-Section Dental Caries Model

Demonstration of a Fluoride Dose Response with an In situ Single-Section Dental Caries Model

Effect of an Alcohol-Free, 1% Chlorhexidine Gel as an Adjunct to a Fluoridated Dentifrice Using an Intraoral Crown Model

Influence of Nail Varnish on the Remineralization of Enamel Single Sections Assessed by Microradiography and Confocal Laser Scanning Microscopy

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